{"created":"2021-03-01T06:10:24.451362+00:00","id":3928,"links":{},"metadata":{"_buckets":{"deposit":"7b46f436-6802-4f71-8ccf-b7fd3f8e542e"},"_deposit":{"created_by":17,"id":"3928","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"3928"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00003928","sets":["499:508:509:512"]},"author_link":["10017","10018","10019"],"item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3-4","bibliographicPageEnd":"113","bibliographicPageStart":"99","bibliographicVolumeNumber":"63","bibliographic_titles":[{"bibliographic_title":"Nagoya Journal of Medical Science","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The catalytic subunit of human DNA polymerase (pol) δ, p125, was expressed in recombinant baculovirus-infected insect cells, separated from a baculovirus-encoded DNA polymerase, and was purified to homogeneity by affinity trapping with a histidine-octapeptide at the C-terminus of p125 as the ligand. Purified p125 showed DNA polymerase activity resembling conventionally purified calf thymus pol δ. However, the two differed in four ways: 1) the specific activity of recombinant p125 was one quarter of the calf thymus pol δ; 2) the recombinant p125 was relatively resistant to aphidicolin; 3) the apparent Km for dTTP of the recombinant p125 was estimated at 33 μM, 15-fold the value for calf thymus pol δ; and 4) the recombinant p125 was not stimulated by recombinant PCNA, while activity of calf thymus pol δ increased 150-fold in response. Furthermore, PCNA did not stimulate either the p125 incubated with p50, a small subunit of pol δ, or co-expressed with p50 in insect cells. The full length recombinant p125 migrated slightly faster than pol δ from human cell lines, Jurkat or HeLa, upon SDS-polyacrylamide gel electrophoresis, suggesting a post-translational modification. The results indicate that in vivo assembly of the fully active complex of pol δ requires factors in addition to p125 and p50 subunits, and/or a post-translational modification of p125.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/5358"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/nagjms.63.3-4.99","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nagoya University School of Medicine","subitem_publisher_language":"en"}]},"item_9_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_9_source_id_61":{"attribute_name":"ISSN（Online）","attribute_value_mlt":[{"subitem_source_identifier":"2186-3326","subitem_source_identifier_type":"EISSN"}]},"item_9_source_id_7":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"0027-7622","subitem_source_identifier_type":"PISSN"}]},"item_9_text_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_text_value":"application/pdf"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Suzuki, Susumu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"10017","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Motoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"10018","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshida, Shonen","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"10019","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-19"}],"displaytype":"detail","filename":"nj6333.pdf","filesize":[{"value":"154.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nj6333.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/3928/files/nj6333.pdf"},"version_id":"b5191919-8b78-4238-a0a9-335f74f0a641"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"DNA polymerase δ catalytic subunit","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA polymerase δ small subunit","subitem_subject_scheme":"Other"},{"subitem_subject":"proliferating cell nuclear antigen","subitem_subject_scheme":"Other"},{"subitem_subject":"recombinant baculovirus","subitem_subject_scheme":"Other"},{"subitem_subject":"aphidicolin","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of the human DNA polymerase of catalyticsubunit expressed by a recombinant baculovirus","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of the human DNA polymerase of catalyticsubunit expressed by a recombinant baculovirus","subitem_title_language":"en"}]},"item_type_id":"9","owner":"17","path":["512"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2006-02-03"},"publish_date":"2006-02-03","publish_status":"0","recid":"3928","relation_version_is_last":true,"title":["Characterization of the human DNA polymerase of catalyticsubunit expressed by a recombinant baculovirus"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-11-16T06:59:17.781256+00:00"}