{"created":"2021-03-01T06:10:27.028259+00:00","id":3969,"links":{},"metadata":{"_buckets":{"deposit":"ca5166ea-fd99-44b6-9aee-391fc6347170"},"_deposit":{"created_by":17,"id":"3969","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"3969"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00003969","sets":["499:508:509:519"]},"author_link":["10166","10167"],"item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-2","bibliographicPageEnd":"44","bibliographicPageStart":"35","bibliographicVolumeNumber":"67","bibliographic_titles":[{"bibliographic_title":"Nagoya Journal of Medical Science","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Human neuroblastomas and gliomas express high levels of GD2 ganglioside. Mechanisms for the re-expression of GD2 after the incorporation of an exogenous precursor structure were analyzed using a human heterophilic monoclonal antibody (mAb) together with mouse anti-GD3 and mouse anti-GD2 mAbs. First, mouse anti-GD2 mAb 220-51 was generated and its reactivity was confirmed to be almost identical with that of the well-known mAb 3F8 antibody. As reported previously for GD3 variants, new ganglioside antigens reactive with human mAb 32-27 were analyzed by culturing an astrocytoma cell line AS in the presence of NeuGc-GM3. Analysis of the extracted gangliosides from AS thus cultured revealed a new component detected with mAb 32-27, migrating similarly to GD2. Incorporated NeuGc-GM3 seemed to be converted to NeuAc-NeuGc-type GD3, and then to NeuAc-NeuGc-type GD2 with α2,8-sialyltransferase and β1,4-GalNAc transferase, respectively. In addition, AS was inoculated into nude mice, and glycolipids were extracted from generated tumors. Analysis of the ganglioside components using mAbs indicated that NeuAc-NeuGc-type GD2 was generated in the xenogeneic tumors by incorporating NeuGc-GM3 from mouse blood.  These results indicated the presence of a pathway for utilization of exogenous gangliosides for remodeling and re-expression in vivo.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/5402"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/nagjms.67.1-2.35","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nagoya University School of Medicine","subitem_publisher_language":"en"}]},"item_9_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_9_source_id_61":{"attribute_name":"ISSN（Online）","attribute_value_mlt":[{"subitem_source_identifier":"2186-3326","subitem_source_identifier_type":"EISSN"}]},"item_9_source_id_7":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"0027-7622","subitem_source_identifier_type":"PISSN"}]},"item_9_text_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_text_value":"application/pdf"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nishio, Masashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"10166","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furukawa, Koichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"10167","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-19"}],"displaytype":"detail","filename":"p35_44.pdf","filesize":[{"value":"235.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"p35_44.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/3969/files/p35_44.pdf"},"version_id":"02d86e44-9f7a-42a4-b7d5-d639426bdafc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Ganglioside","subitem_subject_scheme":"Other"},{"subitem_subject":"NeuG","subitem_subject_scheme":"Other"},{"subitem_subject":"Sialic acid","subitem_subject_scheme":"Other"},{"subitem_subject":"Recycle","subitem_subject_scheme":"Other"},{"subitem_subject":"Glycosyltransferase","subitem_subject_scheme":"Other"},{"subitem_subject":"Astrocytoma","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Incorporation, remodeling and re-expression of exogenous gangliosides in human cancer cell lines in vitro and in vivo","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Incorporation, remodeling and re-expression of exogenous gangliosides in human cancer cell lines in vitro and in vivo","subitem_title_language":"en"}]},"item_type_id":"9","owner":"17","path":["519"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2006-02-10"},"publish_date":"2006-02-10","publish_status":"0","recid":"3969","relation_version_is_last":true,"title":["Incorporation, remodeling and re-expression of exogenous gangliosides in human cancer cell lines in vitro and in vivo"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-11-16T06:43:32.626353+00:00"}