{"created":"2021-03-01T06:10:27.535828+00:00","id":3977,"links":{},"metadata":{"_buckets":{"deposit":"370d8398-69da-4acf-bd97-f14808e502e6"},"_deposit":{"created_by":17,"id":"3977","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"3977"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00003977","sets":["499:508:509:520"]},"author_link":["10202","10203","10204","10205","10206","10207","10208"],"item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3-4","bibliographicPageEnd":"108","bibliographicPageStart":"101","bibliographicVolumeNumber":"67","bibliographic_titles":[{"bibliographic_title":"Nagoya Journal of Medical Science","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Malignant transformation of cells is frequently associated with an augmented production of hyaluronan and the subsequent formation of a hyaluronan-matrix. In v-Src-transformed cells, hyaluronan directly activate cell motility in a tumor-specifi c manner. Despite its importance, the mechanism by which v-Src activates hyaluronan production remains unclear. Here we report that multiple signaling pathways are required for the augmented production of hyaluronan. Either the expression of a dominant negative Ras or the treatment of cells with manumycin A, a Ras farnesyltransferase inhibitor, was able to suppress hyaluronan production. In contrast, expression of MEK1EE, a constitutive form of MEK1, activated both hyaluronan synthase expression and hyaluronan production. AG-490, a Jak-2 inhibitor, or LY294002, a PI3K inhibitor, similarly suppressed the augmented production of hyarulonan. Taken together, our results suggest the involvement of multiple signaling pathways, including Ras-dependent and independent ones, in augmented hyaluronan production by v-Src.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/5410"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/nagjms.67.3-4.101","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nagoya University School of  Medicine","subitem_publisher_language":"en"}]},"item_9_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_9_source_id_61":{"attribute_name":"ISSN（Online）","attribute_value_mlt":[{"subitem_source_identifier":"2186-3326","subitem_source_identifier_type":"EISSN"}]},"item_9_source_id_7":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"0027-7622","subitem_source_identifier_type":"PISSN"}]},"item_9_text_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_text_value":"application/pdf"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Naito, Yuko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"10202","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Noriko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"10203","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Huang, Pengyu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"10204","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hasegawa, Hitoki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"10205","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sohara, Yasuyoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"10206","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Iwamoto, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"10207","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hamaguchi, Michinari","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"10208","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-19"}],"displaytype":"detail","filename":"p101NAITO.pdf","filesize":[{"value":"322.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"p101NAITO.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/3977/files/p101NAITO.pdf"},"version_id":"55f9a774-85cb-4c57-b2e6-ed13f0938500"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Hyaluronan","subitem_subject_scheme":"Other"},{"subitem_subject":"v-Src","subitem_subject_scheme":"Other"},{"subitem_subject":"Signaling","subitem_subject_scheme":"Other"},{"subitem_subject":"Ras","subitem_subject_scheme":"Other"},{"subitem_subject":"MEK1","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Requirement of Multiple Signaling Pathways for the Augmented Production of Hyaluronan by V-SRC","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Requirement of Multiple Signaling Pathways for the Augmented Production of Hyaluronan by V-SRC","subitem_title_language":"en"}]},"item_type_id":"9","owner":"17","path":["520"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2006-02-10"},"publish_date":"2006-02-10","publish_status":"0","recid":"3977","relation_version_is_last":true,"title":["Requirement of Multiple Signaling Pathways for the Augmented Production of Hyaluronan by V-SRC"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-11-16T06:43:54.445081+00:00"}