{"created":"2021-03-01T06:11:26.237247+00:00","id":4905,"links":{},"metadata":{"_buckets":{"deposit":"f7134ecd-6409-4005-b124-3592ecb74095"},"_deposit":{"id":"4905","owners":[],"pid":{"revision_id":0,"type":"depid","value":"4905"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00004905","sets":["643:644:645"]},"author_link":["12223","12224"],"item_12_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-10-31","bibliographicIssueDateType":"Issued"}}]},"item_12_date_granted_64":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"1997-10-31"}]},"item_12_degree_grantor_62":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"名古屋大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"NAGOYA University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"13901","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_12_degree_name_61":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(農学)","subitem_degreename_language":"ja"}]},"item_12_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Peptidal growth factors were isolated from conditioned medium derived from rice (Oriza sativa L.) suspension cultures and identified to be a sulfated pentapeptide(Htyr (SO3H)-Ile-Tyr (SO3H)-Thr-Gln-OH）and its C-terminal truncated tetrapeptide (Htyr (SO3H)-Ile-Tyr(SO3H)-Thr-OH).These structures were identical to the phytosulfokines originally found in asparagus (Asparagus officinalis L.) mesophyll cultures. The pentapeptide phytosulfokine-α(PSK-α) very storongly stimulated colony formation of rice protoplasts at concentrations above 10-8 M, indicating a species-independent mode of action of phytosulfokines. Binding assays using 35S-labeled phytosulfoking sites on the surface of rice cells in susupension. Analysis of [35S]PSK-α binding in differential centrifugation fractions suggested association of the binding with a plasma membrane -enriched fraction. The apparent Kd values for[35S]PSK-αbinding was found to be 1 x 10-9 M for the high affinity type and 1 x 10-7 M for the low affinty type, with maximum numbers of binding sites of 1 x 104 sites/cell and 1 x 105 sites/cell, respectively. Competition studies with the [35S]PSK-αand several synthetic PSK-αanalogs demonstrated that only peptides which possesses mitogenic activity can effectively displace the radioligand. These results suggest that a novel signal transduction pathway mediated by peptidal factors is involved in plant cell proliferation.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_12_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"名古屋大学博士学位論文 学位の種類:博士(農学) (課程) 学位授与年月日:平成9年10月31日","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_12_dissertation_number_65":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第3831号"}]},"item_12_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/6441"}]},"item_12_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_12_text_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_text_value":"application/pdf"}]},"item_12_text_63":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_text_value":"1997"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"松林, 嘉克","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"12223","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsubayashi, Yoshikatsu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"12224","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-19"}],"displaytype":"detail","filename":"ko3831.pdf","filesize":[{"value":"5.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ko3831.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/4905/files/ko3831.pdf"},"version_id":"d4db4426-061d-4245-b433-1d8d25f76306"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"高等植物の細胞増殖因子に関する生物有機化学的研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"高等植物の細胞増殖因子に関する生物有機化学的研究","subitem_title_language":"ja"}]},"item_type_id":"12","owner":"1","path":["645"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2006-05-30"},"publish_date":"2006-05-30","publish_status":"0","recid":"4905","relation_version_is_last":true,"title":["高等植物の細胞増殖因子に関する生物有機化学的研究"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T03:49:30.423619+00:00"}