{"_buckets": {"deposit": "cfbbb558-a8a8-4141-8683-6dd5b1857025"}, "_deposit": {"id": "5109", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "5109"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00005109", "sets": ["667"]}, "author_link": ["12644", "12645", "12646", "12647"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005-07", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "17", "bibliographicPageEnd": "3631", "bibliographicPageStart": "3625", "bibliographicVolumeNumber": "579", "bibliographic_titles": [{"bibliographic_title": "FEBS Letters", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Redox control of disulfide-bond formation in the H+-pyrophosphatase of Streptomyces coelicolor was investigated using cysteine mutants expressed in Escherichia coli. The wildtype enzyme, but not a cysteine-less mutant, was reversibly inactivated by oxidation. To determine the residues involved in oxidative inactivation, different cysteine residues were replaced. Analysis with a cysteine-modifying reagent revealed that the formation of a disulfide bond between cysteines 253 and 621 was responsible for enzyme inactivation. This result suggests that residues in different cytoplasmic loops are close to each other in the tertiary structure. Both cysteine residues are conserved in K+-independent (type II) H+- pyrophosphatases.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/6662"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier", "subitem_publisher_language": "en"}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_10_text_14": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mimura, Hisatoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "12644", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakanishi, Yoichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "12645", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Maeshima, Masayoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "12646", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "前島, 正義", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "12647", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "2005-FEBS_Lett-ScPP.pdf", "filesize": [{"value": "691.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 691900.0, "url": {"label": "2005-FEBS_Lett-ScPP.pdf", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/5109/files/2005-FEBS_Lett-ScPP.pdf"}, "version_id": "84f759a2-f218-4ac9-b531-b9e75bdcfc37"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "H+-PPase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Redox control", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Proton pump", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Streptomyces coelicolor", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Disulfide-bond formation in the H+-pyrophosphatase of Streptomyces coelicolor and its implications for redox control and enzyme structure", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Disulfide-bond formation in the H+-pyrophosphatase of Streptomyces coelicolor and its implications for redox control and enzyme structure", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["667"], "permalink_uri": "http://hdl.handle.net/2237/6662", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2006-06-30"}, "publish_date": "2006-06-30", "publish_status": "0", "recid": "5109", "relation": {}, "relation_version_is_last": true, "title": ["Disulfide-bond formation in the H+-pyrophosphatase of Streptomyces coelicolor and its implications for redox control and enzyme structure"], "weko_shared_id": -1}