{"created":"2021-03-01T06:12:02.722663+00:00","id":5481,"links":{},"metadata":{"_buckets":{"deposit":"22d2fff1-e09a-4375-bec7-90adfb5ce086"},"_deposit":{"id":"5481","owners":[],"pid":{"revision_id":0,"type":"depid","value":"5481"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00005481","sets":["643:666:667"]},"author_link":["14139","14140"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"819","bibliographicPageStart":"812","bibliographicVolumeNumber":"279","bibliographic_titles":[{"bibliographic_title":"Journal of Biological Chemistry","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In plants, the cation/H^+ exchanger translocates Ca^{2+}and other metal ions into vacuoles using the H^+ gradient formed by H^+-ATPase and H^+-pyrophosphatase. Such exchangers carrying 11 transmembrane domains have been isolated from plants, yeast and bacteria. In this study, multiple sequence alignment of several cation/H^+ exchangers revealed the presence of 36 residue highly conserved regions between the 3rd and 4th, and the 8th and 9th transmembrane domains. These two repetitive motifs are designated repeats c-1 and c-2. Using site-directed mutagenesis, we generated 31 mutations in the repeats of the Oryza sativa cation/H^+ exchanger, which translocates Ca^{2+} and Mn^{2+}. Mutant exchangers were expressed in a Saccharomyces cerevisiae strain that was sensitive to Ca^{2+} and Mn^{2+} due to the absence of vacuolar Ca^{2+}-ATPase and the Ca^{2+}/H+ exchanger. Mutant exchangers were classified into 6 classes according to their tolerance to Ca^{2+ }and Mn^{2+}. For example, the class III mutants had no tolerance to either ion and the class IV mutants had tolerance only to Ca^{2+}. The biochemical function of each residue was estimated. We investigated the membrane topology of the repeats using a method combining cysteine mutagenesis with sulfhydryl reagents. Our results suggest that the repeat c-1 re-enters the membrane from the vacuolar lumen side and forms a solution-accessible region. Furthermore, several residues in repeats c-1 and c-2 were found to be conserved in animal Na+/Ca^{2+} exchangers. Finally, we suggest that these re-entrant repeats may form a vestibule or filter for cation selection.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/7088"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"the American Society for Biochemistry and Molecular Biology","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1074/jbc.M309726200","subitem_relation_type_select":"DOI"}}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_10_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-9258","subitem_source_identifier_type":"PISSN"}]},"item_10_text_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_text_value":"application/pdf"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kamiya, Takehiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"14139","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Maeshima, Masayoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"14140","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-19"}],"displaytype":"detail","filename":"2004-JBC-OsCAX.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2004-JBC-OsCAX.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/5481/files/2004-JBC-OsCAX.pdf"},"version_id":"24eebb3a-d4cf-4c38-8c23-e473a9b181f1"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Residues in Internal Repeats of the Rice Cation/H+ Exchanger are Involved in the Transport and Selection of Cations","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Residues in Internal Repeats of the Rice Cation/H+ Exchanger are Involved in the Transport and Selection of Cations","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["667"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2006-10-27"},"publish_date":"2006-10-27","publish_status":"0","recid":"5481","relation_version_is_last":true,"title":["Residues in Internal Repeats of the Rice Cation/H+ Exchanger are Involved in the Transport and Selection of Cations"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T03:54:22.434049+00:00"}