{"_buckets": {"deposit": "c726f3b8-2e6f-429c-a620-5e5c24775a42"}, "_deposit": {"id": "5691", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "5691"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00005691"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1999-10-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "444", "bibliographicPageStart": "437", "bibliographicVolumeNumber": "238", "bibliographic_titles": [{"bibliographic_title": "Gene"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "The Rho family of small GTPases and their associated regulators and targets are essential mediators of diverse morphogenetic events in development. Mammalian Rho\u2010kinase/ROK\u03b1, one of the targets of Rho, has been shown to bind to Rho in GTP\uff0dbound form and to phosphorylate the myosin light chain (MLC) and the myosin binding subunit (MBS) of myosin phosphatase, resulting in the activation of myosin. Thus, Rho\u2010kinase/ROK\u03b1 has been suggested to play essential roles in the formation of stress fibers and focal adhesions. We have identified the Drosophila homolog of Rho\u2010kinase/ROK\u03b1, DRho\u2010kinase, which has conserved the basic structural feature of Rho\u2010kinase/ROK\u03b1 consisting of the N\u2010terminal kinase, central coiled-coil and C\u2010terminal pleckstrin homology (PH) domains. A two\u2010hybrid analysis demonstrated that DRho\u2010kinase interacts with the GTP\u2010bound form of the Drosophila Rho, Drho1, at the conserved Rho-binding site. DRho\u2010kinase can phosphorylate MLC and MBS, preferable substrates for bovine Rho\u2010kinase, in vitro. DRho\u2010kinase is ubiquitously expressed throughout development, in a pattern essentially identical to that of Drho1. These results suggest that DRho\u2010kinase is an effector of Drho1.", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/7297"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://dx.doi.org/10.1016/S0378-1119(99)00351-0", "subitem_relation_type_select": "DOI"}}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0378-1119", "subitem_source_identifier_type": "ISSN"}]}, "item_10_text_14": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mizuno, Tomoaki"}], "nameIdentifiers": [{"nameIdentifier": "15134", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Amano, Mutsuki"}], "nameIdentifiers": [{"nameIdentifier": "15135", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kaibuchi, Kozo"}], "nameIdentifiers": [{"nameIdentifier": "15136", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishida, Yasuyoshi"}], "nameIdentifiers": [{"nameIdentifier": "15137", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "DRho_kinase_final.pdf", "filesize": [{"value": "417.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 417500.0, "url": {"label": "DRho_kinase_final.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/5691/files/DRho_kinase_final.pdf"}, "version_id": "52ab54d5-fd45-45ec-a6d6-4c5343dde905"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Rho effector", "subitem_subject_scheme": "Other"}, {"subitem_subject": "actin cytoskeleton", "subitem_subject_scheme": "Other"}, {"subitem_subject": "morphogenesis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "low stringency PCR", "subitem_subject_scheme": "Other"}, {"subitem_subject": "two-hybrid analysis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "in vitro kinase assay", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Identification and characterization of Drosophila homolog of Rho-kinase", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Identification and characterization of Drosophila homolog of Rho-kinase"}]}, "item_type_id": "10", "owner": "1", "path": ["336/695/696"], "permalink_uri": "http://hdl.handle.net/2237/7297", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2007-02-09"}, "publish_date": "2007-02-09", "publish_status": "0", "recid": "5691", "relation": {}, "relation_version_is_last": true, "title": ["Identification and characterization of Drosophila homolog of Rho-kinase"], "weko_shared_id": 3}