WEKO3
アイテム
{"_buckets": {"deposit": "c726f3b8-2e6f-429c-a620-5e5c24775a42"}, "_deposit": {"id": "5691", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "5691"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00005691", "sets": ["696"]}, "author_link": ["15134", "15135", "15136", "15137"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1999-10-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "444", "bibliographicPageStart": "437", "bibliographicVolumeNumber": "238", "bibliographic_titles": [{"bibliographic_title": "Gene", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The Rho family of small GTPases and their associated regulators and targets are essential mediators of diverse morphogenetic events in development. Mammalian Rho‐kinase/ROKα, one of the targets of Rho, has been shown to bind to Rho in GTP-bound form and to phosphorylate the myosin light chain (MLC) and the myosin binding subunit (MBS) of myosin phosphatase, resulting in the activation of myosin. Thus, Rho‐kinase/ROKα has been suggested to play essential roles in the formation of stress fibers and focal adhesions. We have identified the Drosophila homolog of Rho‐kinase/ROKα, DRho‐kinase, which has conserved the basic structural feature of Rho‐kinase/ROKα consisting of the N‐terminal kinase, central coiled-coil and C‐terminal pleckstrin homology (PH) domains. A two‐hybrid analysis demonstrated that DRho‐kinase interacts with the GTP‐bound form of the Drosophila Rho, Drho1, at the conserved Rho-binding site. DRho‐kinase can phosphorylate MLC and MBS, preferable substrates for bovine Rho‐kinase, in vitro. DRho‐kinase is ubiquitously expressed throughout development, in a pattern essentially identical to that of Drho1. These results suggest that DRho‐kinase is an effector of Drho1.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/7297"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier", "subitem_publisher_language": "en"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/S0378-1119(99)00351-0", "subitem_relation_type_select": "DOI"}}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0378-1119", "subitem_source_identifier_type": "PISSN"}]}, "item_10_text_14": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mizuno, Tomoaki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "15134", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Amano, Mutsuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "15135", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kaibuchi, Kozo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "15136", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishida, Yasuyoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "15137", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "DRho_kinase_final.pdf", "filesize": [{"value": "417.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 417500.0, "url": {"label": "DRho_kinase_final.pdf", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/5691/files/DRho_kinase_final.pdf"}, "version_id": "52ab54d5-fd45-45ec-a6d6-4c5343dde905"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Rho effector", "subitem_subject_scheme": "Other"}, {"subitem_subject": "actin cytoskeleton", "subitem_subject_scheme": "Other"}, {"subitem_subject": "morphogenesis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "low stringency PCR", "subitem_subject_scheme": "Other"}, {"subitem_subject": "two-hybrid analysis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "in vitro kinase assay", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Identification and characterization of Drosophila homolog of Rho-kinase", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Identification and characterization of Drosophila homolog of Rho-kinase", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["696"], "permalink_uri": "http://hdl.handle.net/2237/7297", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2007-02-09"}, "publish_date": "2007-02-09", "publish_status": "0", "recid": "5691", "relation": {}, "relation_version_is_last": true, "title": ["Identification and characterization of Drosophila homolog of Rho-kinase"], "weko_shared_id": -1}
Identification and characterization of Drosophila homolog of Rho-kinase
http://hdl.handle.net/2237/7297
http://hdl.handle.net/2237/729728a6ce2d-1402-441c-bea9-691100844b87
名前 / ファイル | ライセンス | アクション |
---|---|---|
![]() |
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2007-02-09 | |||||
タイトル | ||||||
タイトル | Identification and characterization of Drosophila homolog of Rho-kinase | |||||
言語 | en | |||||
著者 |
Mizuno, Tomoaki
× Mizuno, Tomoaki× Amano, Mutsuki× Kaibuchi, Kozo× Nishida, Yasuyoshi |
|||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Rho effector | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | actin cytoskeleton | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | morphogenesis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | low stringency PCR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | two-hybrid analysis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | in vitro kinase assay | |||||
抄録 | ||||||
内容記述 | The Rho family of small GTPases and their associated regulators and targets are essential mediators of diverse morphogenetic events in development. Mammalian Rho‐kinase/ROKα, one of the targets of Rho, has been shown to bind to Rho in GTP-bound form and to phosphorylate the myosin light chain (MLC) and the myosin binding subunit (MBS) of myosin phosphatase, resulting in the activation of myosin. Thus, Rho‐kinase/ROKα has been suggested to play essential roles in the formation of stress fibers and focal adhesions. We have identified the Drosophila homolog of Rho‐kinase/ROKα, DRho‐kinase, which has conserved the basic structural feature of Rho‐kinase/ROKα consisting of the N‐terminal kinase, central coiled-coil and C‐terminal pleckstrin homology (PH) domains. A two‐hybrid analysis demonstrated that DRho‐kinase interacts with the GTP‐bound form of the Drosophila Rho, Drho1, at the conserved Rho-binding site. DRho‐kinase can phosphorylate MLC and MBS, preferable substrates for bovine Rho‐kinase, in vitro. DRho‐kinase is ubiquitously expressed throughout development, in a pattern essentially identical to that of Drho1. These results suggest that DRho‐kinase is an effector of Drho1. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Elsevier | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/S0378-1119(99)00351-0 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0378-1119 | |||||
書誌情報 |
en : Gene 巻 238, 号 2, p. 437-444, 発行日 1999-10-01 |
|||||
フォーマット | ||||||
application/pdf | ||||||
著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/7297 | |||||
識別子タイプ | HDL |