WEKO3
アイテム
Identification and characterization of Drosophila homolog of Rho-kinase
http://hdl.handle.net/2237/7297
http://hdl.handle.net/2237/729728a6ce2d-1402-441c-bea9-691100844b87
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
DRho_kinase_final.pdf (417.5 kB)
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-02-09 | |||||
| タイトル | ||||||
| タイトル | Identification and characterization of Drosophila homolog of Rho-kinase | |||||
| 言語 | en | |||||
| 著者 |
Mizuno, Tomoaki
× Mizuno, Tomoaki× Amano, Mutsuki× Kaibuchi, Kozo× Nishida, Yasuyoshi |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Rho effector | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | actin cytoskeleton | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | morphogenesis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | low stringency PCR | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | two-hybrid analysis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | in vitro kinase assay | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The Rho family of small GTPases and their associated regulators and targets are essential mediators of diverse morphogenetic events in development. Mammalian Rho‐kinase/ROKα, one of the targets of Rho, has been shown to bind to Rho in GTP－bound form and to phosphorylate the myosin light chain (MLC) and the myosin binding subunit (MBS) of myosin phosphatase, resulting in the activation of myosin. Thus, Rho‐kinase/ROKα has been suggested to play essential roles in the formation of stress fibers and focal adhesions. We have identified the Drosophila homolog of Rho‐kinase/ROKα, DRho‐kinase, which has conserved the basic structural feature of Rho‐kinase/ROKα consisting of the N‐terminal kinase, central coiled-coil and C‐terminal pleckstrin homology (PH) domains. A two‐hybrid analysis demonstrated that DRho‐kinase interacts with the GTP‐bound form of the Drosophila Rho, Drho1, at the conserved Rho-binding site. DRho‐kinase can phosphorylate MLC and MBS, preferable substrates for bovine Rho‐kinase, in vitro. DRho‐kinase is ubiquitously expressed throughout development, in a pattern essentially identical to that of Drho1. These results suggest that DRho‐kinase is an effector of Drho1. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/S0378-1119(99)00351-0 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0378-1119 | |||||
| 書誌情報 |
en : Gene 巻 238, 号 2, p. 437-444, 発行日 1999-10-01 |
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| フォーマット | ||||||
| 値 | application/pdf | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/7297 | |||||
| 識別子タイプ | HDL | |||||
