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  1. C100 医学部/医学系研究科
  2. C100a 雑誌掲載論文
  3. 学術雑誌

New monoclonal antibody (AIC) identifies interstitial cells of Cajal in the musculature of the mouse gastrointestinal tract

http://hdl.handle.net/2237/7445
http://hdl.handle.net/2237/7445
4f2842c7-a9d6-4959-a562-1b3ff845e8e6
名前 / ファイル ライセンス アクション
Table1.pdf Table1.pdf (46.5 kB)
Fig6.pdf Fig6.pdf (59.4 kB)
Fig5.pdf Fig5.pdf (33.5 kB)
Fig4.pdf Fig4.pdf (39.2 kB)
Fig3.pdf Fig3.pdf (30.1 kB)
Fig2.pdf Fig2.pdf (32.4 kB)
Fig1.pdf Fig1.pdf (73.2 kB)
Accepted.pdf Accepted.pdf (137.6 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2007-03-07
タイトル
タイトル New monoclonal antibody (AIC) identifies interstitial cells of Cajal in the musculature of the mouse gastrointestinal tract
言語 en
著者 Torihashi, S.

× Torihashi, S.

WEKO 15475

en Torihashi, S.

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Yokoi, K.

× Yokoi, K.

WEKO 15476

en Yokoi, K.

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Nagaya, H.

× Nagaya, H.

WEKO 15477

en Nagaya, H.

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Aoki, K.

× Aoki, K.

WEKO 15478

en Aoki, K.

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Fujimoto, T.

× Fujimoto, T.

WEKO 15479

en Fujimoto, T.

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鳥橋, 茂子

× 鳥橋, 茂子

WEKO 15480

ja 鳥橋, 茂子

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 This is the author’s version of a work accepted for publication by Elsevier. Changes resulting from the publishing process, including peer review, editing, corrections, structural formatting and other quality control mechanisms, may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Autonomic Neuroscience-Basic & Clinical. v.113, n.1-2, 2004, http://dx.doi.org/10.1016/j.autneu.2004.05.004
抄録
内容記述 Interstitial cells of Cajal (ICC) are pacemaker cells for the spontaneous muscular contractions and neuromodulators that mediate neurotransmission from enteric neurons to smooth muscle cells in the gastrointestinal (GI) tract. They express c-Kit, and the antibody for c-Kit (especially ACK2) has been a useful tool for functional and morphological studies. ACK2, however, does not work on tissues fixed with paraformaldehyde and not all ICC express c-Kit in human. Therefore, in order to find a new marker of ICC and/or new antibody resisting aldehyde fixation we produced a new monoclonal antibody that identifies ICC and then investigated the properties of its antigen. Isolated ICC were used for immunization. Hybridomas fused with myeloma SP2 were screened by immunohistochemistry. ACK2 and each antibody were applied on serial sections, and the clone producing antibody AIC that stains ICC was established. The distribution of AIC immunopositive cells were examined in other organs and also GI muscles of W/Wv mice. The biochemical properties were studied using dot blot analysis. AIC recognized ICC, however, distribution of immunopositive cells in W/Wv mice and other organs was different from that of c-Kit. The immunoreactivity was stable for paraformaldehyde but was blocked by either Triton X-100 or SDS. In conclusion new antibody AIC recognized ICC but the antigen was not c-Kit, which confirms the existence of good markers of ICC besides c-Kit. Although the antigen has not been isolated, AIC is suitable for morphological study and useful for investigation of ICC in c-Kit mutants.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.autneu.2004.05.004
ISSN
収録物識別子タイプ PISSN
収録物識別子 1566-0702
書誌情報 en : Autonomic Neuroscience-Basic & Clinical

巻 113, 号 1-2, p. 16-23, 発行日 2004-06-30
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application/pdf
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application/pdf
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著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/7445
識別子タイプ HDL
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