@article{oai:nagoya.repo.nii.ac.jp:00005829,
 author = {Torihashi, S. and Yokoi, K. and Nagaya, H. and Aoki, K. and Fujimoto, T. and 鳥橋, 茂子},
 issue = {1-2},
 journal = {Autonomic Neuroscience-Basic & Clinical},
 month = {Jun},
 note = {Interstitial cells of Cajal (ICC) are pacemaker cells for the spontaneous muscular contractions and neuromodulators that mediate neurotransmission from enteric neurons to smooth muscle cells in the gastrointestinal (GI) tract. They express c-Kit, and the antibody for c-Kit (especially ACK2) has been a useful tool for functional and morphological studies. ACK2, however, does not work on tissues fixed with paraformaldehyde and not all ICC express c-Kit in human. Therefore, in order to find a new marker of ICC and/or new antibody resisting aldehyde fixation we produced a new monoclonal antibody that identifies ICC and then investigated the properties of its antigen. Isolated ICC were used for immunization. Hybridomas fused with myeloma SP2 were screened by immunohistochemistry.  ACK2 and each antibody were applied on serial sections, and the clone producing antibody AIC that stains ICC was established. The distribution of AIC immunopositive cells were examined in other organs and also GI muscles of W/Wv mice. The biochemical properties were studied using dot blot analysis. AIC recognized ICC, however, distribution of immunopositive cells in W/Wv mice and other organs was different from that of c-Kit. The immunoreactivity was stable for paraformaldehyde but was blocked by either Triton X-100 or SDS. In conclusion new antibody AIC recognized ICC but the antigen was not c-Kit, which confirms the existence of good markers of ICC besides c-Kit. Although the antigen has not been isolated, AIC is suitable for morphological study and useful for investigation of ICC in c-Kit mutants.},
 pages = {16--23},
 title = {New monoclonal antibody (AIC) identifies interstitial cells of Cajal in the musculature of the mouse gastrointestinal tract},
 volume = {113},
 year = {2004}
}