{"created":"2021-03-01T06:12:24.765067+00:00","id":5831,"links":{},"metadata":{"_buckets":{"deposit":"37114338-fa69-41f9-862a-ec8894def859"},"_deposit":{"id":"5831","owners":[],"pid":{"revision_id":0,"type":"depid","value":"5831"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00005831","sets":["499:500:501"]},"author_link":["15488","15489","15490","15491","15492"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"21","bibliographicPageEnd":"19197","bibliographicPageStart":"19191","bibliographicVolumeNumber":"277","bibliographic_titles":[{"bibliographic_title":"Journal of Biological Chemistry","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Interstitial cells of Cajal (ICC) are considered to be pacemaker cells in gastrointestinal tracts. ICC generate rhythmical electronic activity as slow waves, which is dihydropyridine-insensitive, and drive spontaneous contraction of smooth muscles. Although cytosolic Ca2+ has been assumed to play a key role in pacemaking, Ca2+ movements in ICC have not yet been examined in detail. In the present study, using cultured cell clusters isolated from the mouse small intestine, we demonstrated Ca2+ oscillations in ICC. Fluo4 was loaded to the cell cluster, the relative amount of cytosolic Ca2+ was recorded, and ICC were identified by c-Kit immunoreactivity. We specifically detected Ca2+ oscillation in ICC in the presence of dihydropyridine, which abolishes Ca2+ oscillation in smooth muscles. The oscillation was coupled to the electrical activity corresponding to slow waves and depended on Ca2+ influx through a non-selective cation channel, which was SK&F 96365-sensitive and store-operated. We further demonstrated the presence of transient receptor potential-like channel 4 (TRP4) in caveolae of ICC. Taken together, the results infer that the Ca2+ oscillation in ICC is intimately linked to the pacemaker function and depends on Ca2+ influx mediated by TRP4.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/7447"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The American Society for Biochemistry and Molecular Biology","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1074/jbc.M201728200","subitem_relation_type_select":"DOI"}}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_10_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-9258","subitem_source_identifier_type":"PISSN"}]},"item_10_text_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_text_value":"application/pdf"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Torihashi, Shigeko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"15488","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimoto, Toyoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"15489","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Trost, Claudia","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"15490","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakayama, Shinsuke","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"15491","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鳥橋, 茂子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"15492","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-19"}],"displaytype":"detail","filename":"M2-01728a.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"M2-01728a.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/5831/files/M2-01728a.pdf"},"version_id":"712d588d-9131-4994-9feb-970f576a3e34"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Calcium oscillation linked to pacemaking of interstitial cells of Cajal;Requirement of calcium influx and localisation of TRP4 in caveolae","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Calcium oscillation linked to pacemaking of interstitial cells of Cajal;Requirement of calcium influx and localisation of TRP4 in caveolae","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["501"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-03-08"},"publish_date":"2007-03-08","publish_status":"0","recid":"5831","relation_version_is_last":true,"title":["Calcium oscillation linked to pacemaking of interstitial cells of Cajal;Requirement of calcium influx and localisation of TRP4 in caveolae"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:54:33.378678+00:00"}