{"created":"2021-03-01T06:12:32.914782+00:00","id":5961,"links":{},"metadata":{"_buckets":{"deposit":"6996033f-f8ae-496d-b93e-5d6ef1ba053b"},"_deposit":{"id":"5961","owners":[],"pid":{"revision_id":0,"type":"depid","value":"5961"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00005961"},"item_9_biblio_info_6":{"attribute_name":"\u66f8\u8a8c\u60c5\u5831","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-12","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"44","bibliographicPageStart":"42","bibliographicVolumeNumber":"47","bibliographic_titles":[{"bibliographic_title":"Environmental Medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University"}]}]},"item_9_description_4":{"attribute_name":"\u6284\u9332","attribute_value_mlt":[{"subitem_description":"T-type Ca^2+ channels are particularly abundant in neuronal tissue and immature cardiac heart and mediate burst firing and pacemaker activity. Northern blot analyses have demonstrated that one subtype of T-type Ca^2+ channels, Ca_3.1(\u03b11G), mRNA is predominantly expressed in adult brain and immature heart. However, the protein expression of Ca_3.1 channels is not clear, since the anti-Ca_3.1 ; sc-16259 and sc-16260, we performed Western blot analysis of adult brain and embryonic heart of mouse. Sc-16259 and sc-16260 may not be suitable for detection of Ca_3.1 channel protein in mouse tissue samples.","subitem_description_type":"Abstract"}]},"item_9_description_5":{"attribute_name":"\u5185\u5bb9\u8a18\u8ff0","attribute_value_mlt":[{"subitem_description":"\u56fd\u7acb\u60c5\u5831\u5b66\u7814\u7a76\u6240\u3067\u96fb\u5b50\u5316\u3057\u305f\u30b3\u30f3\u30c6\u30f3\u30c4\u3092\u4f7f\u7528\u3057\u3066\u3044\u308b\u3002","subitem_description_type":"Other"}]},"item_9_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/7575"}]},"item_9_identifier_registration":{"attribute_name":"ID\u767b\u9332","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/envm.47.42","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"\u51fa\u7248\u8005","attribute_value_mlt":[{"subitem_publisher":"Research Institute of Environmental Medicine, Nagoya University"}]},"item_9_select_15":{"attribute_name":"\u8457\u8005\u7248\u30d5\u30e9\u30b0","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_9_source_id_7":{"attribute_name":"ISSN\uff08print\uff09","attribute_value_mlt":[{"subitem_source_identifier":"0287-0517","subitem_source_identifier_type":"ISSN"}]},"item_9_text_14":{"attribute_name":"\u30d5\u30a9\u30fc\u30de\u30c3\u30c8","attribute_value_mlt":[{"subitem_text_value":"application/pdf"}]},"item_creator":{"attribute_name":"\u8457\u8005","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Niwa, Noriko"}],"nameIdentifiers":[{"nameIdentifier":"15876","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yasui, Kenji"}],"nameIdentifiers":[{"nameIdentifier":"15877","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hojo, Mayumi"}],"nameIdentifiers":[{"nameIdentifier":"15878","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kodama, Itsuo"}],"nameIdentifiers":[{"nameIdentifier":"15879","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"\u30d5\u30a1\u30a4\u30eb\u60c5\u5831","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-19"}],"displaytype":"detail","filename":"42-44.pdf","filesize":[{"value":"273.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"42-44.pdf","url":"https://nagoya.repo.nii.ac.jp/record/5961/files/42-44.pdf"},"version_id":"849d3695-1075-43e2-98e0-3f972c83c865"}]},"item_keyword":{"attribute_name":"\u30ad\u30fc\u30ef\u30fc\u30c9","attribute_value_mlt":[{"subitem_subject":"Ca_3.1","subitem_subject_scheme":"Other"},{"subitem_subject":"T-type Ca^2+ channel","subitem_subject_scheme":"Other"},{"subitem_subject":"Western blot anaysis","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"\u8a00\u8a9e","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"\u8cc7\u6e90\u30bf\u30a4\u30d7","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Immunoblotting of T-type Ca^2+ Channel Protein in Mouse Brain and Embryonic Heart by Using Two Antibodies against Ca_3.1 Channels","item_titles":{"attribute_name":"\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_title":"Immunoblotting of T-type Ca^2+ Channel Protein in Mouse Brain and Embryonic Heart by Using Two Antibodies against Ca_3.1 Channels"}]},"item_type_id":"9","owner":"1","path":["329/333/334/737"],"pubdate":{"attribute_name":"\u516c\u958b\u65e5","attribute_value":"2007-04-05"},"publish_date":"2007-04-05","publish_status":"0","recid":"5961","relation_version_is_last":true,"title":["Immunoblotting of T-type Ca^2+ Channel Protein in Mouse Brain and Embryonic Heart by Using Two Antibodies against Ca_3.1 Channels"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2021-03-01T20:19:21.890635+00:00"}