{"created":"2021-03-01T06:13:29.480776+00:00","id":6862,"links":{},"metadata":{"_buckets":{"deposit":"8add6c9f-2231-406f-a6c4-b0bf776c6601"},"_deposit":{"id":"6862","owners":[],"pid":{"revision_id":0,"type":"depid","value":"6862"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00006862","sets":["643:835:836:845"]},"author_link":["18135","18136","18137","18138","18139","18140"],"item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_9_alternative_title_19":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"スギ成熟木部の生化学的研究 : 心材抽出成分の生合成に関与する酵素の検出の試み","subitem_alternative_title_language":"ja"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-12","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"6","bibliographicPageStart":"1","bibliographicVolumeNumber":"24","bibliographic_titles":[{"bibliographic_title":"名古屋大学森林科学研究","bibliographic_titleLang":"ja"}]}]},"item_9_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"これまで樹木成熟木部の生化学的な研究は少ない。成熟木部が行う重要な生理活動の一つとして心材形成が挙げられる。一般に心材には、辺材には存在しない特徴的な抽出成分が含まれる。本研究では、心材成分生合成の観点からスギ成熟木部の生理的活性を生化学的に調査した。まず、辺材、移行材および心材から凍結木粉を調製した。これら木粉を緩衝液抽出し、その抽出液からタンパク質を硫安沈澱させた。タンパク質量を比色定量した結果、移行材において抽出成分の生成を反映するタンパク質量の増加は認められなかった。タンパク質組成を電気泳動によって調査した結果、移行材において抽出成分の生成を反映するタンパク質の質的変化は認められなかった。一方、スギ心材成分の生合成に関与すると考えられるフェニルアラニンアンモリアリアーゼの活性をガスクロマトグラフ質量分析法によって測定した結果、この酵素が移行材において活性化されることが確認された。したがって、凍結木粉が成熟木部の生化学的な調査に有用であることが示され、これを使用することによって心材成分生合成に関与するスギ成熟木部の生理的活性を検出することができた。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"Many biochemical actions occur in mature tree xylem after cell differentiation is completed in the differentiating xylem. However, biochemical studies of the mature xylem are scarce. One of the most remarkable actions of mature tree xylem is the process of changing sapwood to heartwood, so-called heartwood formation. Generally; heartwood contains particular organic solvent extractable substances called heartwood extractives. In this study we attempted to investigate the biochemical abilities of mature sugi xylem from the perspective of heartwood extractive formation. First, frozen woodpowders were prepared from various parts ofthe xylem. These powders were subjected to buffer extraction, and crude protein extracts were obtained by ammonium sulfate precipitation. The protein contents were determined colorimetrically, and no increase of protein content corresponding to formation of the heartwood extractives in the intermediate wood was found. Protein constitutions were also examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and no alteration of the constitutions corresponding to formation of the extractives in the intermediate wood was found. Conversely; the activity of phenylalanine ammonia-lyase, an enzyme that would be involved in the biosyntheses of sugi heartwood extractives, was determined by gas chromatography-mass spectrometry and was significantly increased in the intermediate wood. Consequently; frozen wood powder is useful for biochemical studies of mature tree xylem, and by using the powder detection of the bioability of mature xylem concerning formation of sugi heartwood extractives was achieved.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"農林水産研究情報センターで作成したPDFファイルを使用している。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_9_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/8529"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.18999/nagufs.24.1","subitem_identifier_reg_type":"JaLC"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"名古屋大学農学部附属演習林","subitem_publisher_language":"ja"}]},"item_9_relation_43":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://rms1.agsearch.agropedia.affrc.go.jp/contents/JASI/pdf/academy/72-3740.pdf","subitem_relation_type_select":"URI"}}]},"item_9_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_9_source_id_7":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"1344-2457","subitem_source_identifier_type":"PISSN"}]},"item_9_text_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_text_value":"application/pdf"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"IMAI, Takanori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"18135","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井, 貴規","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"18136","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ITO, Eriko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"18137","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伊藤, 恵理子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"18138","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"FUKUSHIMA, Kazuhiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"18139","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"福島, 和彦","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"18140","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-19"}],"displaytype":"detail","filename":"nagufs_24_1.pdf","filesize":[{"value":"574.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nagufs_24_1.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/6862/files/nagufs_24_1.pdf"},"version_id":"11cc8933-d3f7-4bd5-8937-9948014a9bb2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"biochemistry","subitem_subject_scheme":"Other"},{"subitem_subject":"Cryptomeria japonica","subitem_subject_scheme":"Other"},{"subitem_subject":"heartwood extractives","subitem_subject_scheme":"Other"},{"subitem_subject":"mature xylem","subitem_subject_scheme":"Other"},{"subitem_subject":"norlignan","subitem_subject_scheme":"Other"},{"subitem_subject":"生化学","subitem_subject_scheme":"Other"},{"subitem_subject":"心材成分","subitem_subject_scheme":"Other"},{"subitem_subject":"成熟木部","subitem_subject_scheme":"Other"},{"subitem_subject":"ノルリグナン","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Biochemical studies of matured xylem of Cryptomeria japonica : Attempts to detect the enzymes involved in the biosyntheses of the heartwood extractives","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Biochemical studies of matured xylem of Cryptomeria japonica : Attempts to detect the enzymes involved in the biosyntheses of the heartwood extractives","subitem_title_language":"en"}]},"item_type_id":"9","owner":"1","path":["845"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-07-12"},"publish_date":"2007-07-12","publish_status":"0","recid":"6862","relation_version_is_last":true,"title":["Biochemical studies of matured xylem of Cryptomeria japonica : Attempts to detect the enzymes involved in the biosyntheses of the heartwood extractives"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T03:52:17.669712+00:00"}