{"_buckets": {"deposit": "b6dc6a90-631a-44de-9a11-7efcf0cbc3df"}, "_deposit": {"id": "7076", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "7076"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00007076", "sets": ["322"]}, "author_link": ["19085", "19086", "19087", "19088", "19089"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "12", "bibliographicPageEnd": "1679", "bibliographicPageStart": "1673", "bibliographicVolumeNumber": "20", "bibliographic_titles": [{"bibliographic_title": "Analytical Sciences", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "A new isoelectric focusing (IEF) system for two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) has been proposed. In this system, a super-soft and tough IEF gel was achieved by casting polyacrylamide gel down to 2.0% T using a loose multifilament string (LMS) of nylon as a gel support. The IEF apparatus for the LMS-gel, fabricated from acrylic boards, had a cooling water chamber, and eliminated the need of electrode solutions by directly connecting the two ends of individual gels to platinum electrodes. The carrier ampholyte-generated pH gradients using the new IEF system was stable over a long duration of time and a wide range of voltages, and the IEF time became shorter using a 2.0% T gel than using a 4.0% T gel. Also, the LMS-gels prepared in different runs exhibited excellent reproducibility. The new IEF system was applied to 2-D PAGE of a chicken skeletal muscle extract, and it was found that the protein loading capacity, protein entry into the LMS-gels, and protein transfer efficiency from the first-dimensional to the second-dimensional gels were significantly improved by using a low-concentration (2.5% T) gel. Also, proteins of high molecular weight of more than 200 kDa were observed in the 2-D maps, and therefore the new IEF system has a very good potential to be applied for fast 2-D PAGE of high molecular-weight proteins.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/8750"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "日本分析化学会", "subitem_publisher_language": "ja"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.2116/analsci.20.1673", "subitem_relation_type_select": "DOI"}}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0910-6340", "subitem_source_identifier_type": "PISSN"}]}, "item_10_text_14": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "LI, Jinxiang", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "19085", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "OGASAWARA, Ayaka", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "19086", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ODAKE, Tamao", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "19087", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "UMEMURA, Tomonari", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "19088", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "TSUNODA, Kin-ichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "19089", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "v20_n12_1673-1679.pdf", "filesize": [{"value": "819.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 819000.0, "url": {"label": "v20_n12_1673-1679.pdf", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/7076/files/v20_n12_1673-1679.pdf"}, "version_id": "229fa0e8-4331-4884-a5ae-3e903e4600b7"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "A New Isoelectric Focusing System for Fast Two-Dimensional Gel Electrophoresis Using a Low-Concentration Polyacrylamide Gel Supported by a Loose Multifilament String", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "A New Isoelectric Focusing System for Fast Two-Dimensional Gel Electrophoresis Using a Low-Concentration Polyacrylamide Gel Supported by a Loose Multifilament String", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["322"], "permalink_uri": "http://hdl.handle.net/2237/8750", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2007-09-14"}, "publish_date": "2007-09-14", "publish_status": "0", "recid": "7076", "relation": {}, "relation_version_is_last": true, "title": ["A New Isoelectric Focusing System for Fast Two-Dimensional Gel Electrophoresis Using a Low-Concentration Polyacrylamide Gel Supported by a Loose Multifilament String"], "weko_shared_id": -1}