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Essential roles of myosin phosphatase in the maintenance of epithelial cell integrity of Drosophila imaginal disc cells
http://hdl.handle.net/2237/9388
http://hdl.handle.net/2237/9388e3d3d311-1239-4f0f-9c1b-88ab0ebbd4b1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
DevBiol-DMBS.pdf (688.9 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-02-01 | |||||
| タイトル | ||||||
| タイトル | Essential roles of myosin phosphatase in the maintenance of epithelial cell integrity of Drosophila imaginal disc cells | |||||
| 言語 | en | |||||
| 著者 |
MITONAKA, Tomoaki
× MITONAKA, Tomoaki× MURAMATSU, Yoshiyuki× SUGIYAMA, Shin× MIZUNO, Tomoaki× NISHIDA, Yasuyoshi |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | This is the author’s version of a work accepted for publication by Elsevier. Changes resulting from the publishing process, including peer review, editing, corrections, structural formatting and other quality control mechanisms, may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Developmental biology. v.309, 2007, p.78-86, doi:10.1016/j.ydbio.2007.06.021 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Myosin II | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | MBS | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | apical-basal polarity | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | motility | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | epithelial morphology | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Reorganization of the actin cytoskeleton and contraction of actomyosin play pivotal roles in controlling cell shape changes and motility in epithelial morphogenesis. Dephosphorylation of the myosin regulatory light chain (MRLC) by myosin phosphatase is one of the key events involved. Allelic combinations producing intermediate strength mutants of the Drosophila myosin-binding subunit (DMBS) of myosin phosphatase showed imaginal discs with multilayered disrupted morphologies, and extremely mislocated cells, suggesting that DMBS is required to maintain proper epithelial organization. Clonal analyses revealed that DMBS null mutant cells appear to retract basally and localization of apical junction markers such as DE-cadherin are indetectable in most cells, whereas phosphorylated MRLC and F-actin become heavily concentrated apically, indicating misconfiguration of the apical cytoskeleton. In agreement with these findings, DMBS was found to concentrate at the apical domain suggesting its function is localized. Phenotypes similar to DMBS mutants including increased migration of cells were obtained by overexpressing the constitutive active form of MRLC or Rho-associated kinase signifying that the phenotypes are indeed caused through activation of Myosin II. The requirement of DMBS for the integrity of static epithelial cells in imaginal discs suggests that the regulation of Myosin II by DMBS has a role more general than its previously demonstrated functions in morphogenetic events. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/j.ydbio.2007.06.021 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0012-1606 | |||||
| 書誌情報 |
en : Developmental biology 巻 309, p. 78-86, 発行日 2007 |
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| フォーマット | ||||||
| 値 | application/pdf | |||||
| 著者版フラグ | ||||||
| 値 | author | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/9388 | |||||
| 識別子タイプ | HDL | |||||
