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  1. C100 医学部/医学系研究科
  2. C100b 紀要
  3. Nagoya journal of medical science
  4. 71(1-2)

THE INDUCTION OF DENTIN BRIDGE-LIKE STRUCTURES BY CONSTRUCTS OF SUBCULTURED DENTAL PULP-DERIVED CELLS AND POROUS HA/TCP IN PORCINE TEETH

https://doi.org/10.18999/nagjms.71.1-2.51
https://doi.org/10.18999/nagjms.71.1-2.51
faa75f31-a118-458b-a8a7-316953aea005
名前 / ファイル ライセンス アクション
p051-062_Ando.pdf p051-062_Ando.pdf (886.8 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2009-03-19
タイトル
タイトル THE INDUCTION OF DENTIN BRIDGE-LIKE STRUCTURES BY CONSTRUCTS OF SUBCULTURED DENTAL PULP-DERIVED CELLS AND POROUS HA/TCP IN PORCINE TEETH
著者 ANDO, YUSUKE

× ANDO, YUSUKE

WEKO 27560

ANDO, YUSUKE

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HONDA, MASAKI J.

× HONDA, MASAKI J.

WEKO 27561

HONDA, MASAKI J.

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OHSHIMA, HAYATO

× OHSHIMA, HAYATO

WEKO 27562

OHSHIMA, HAYATO

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TONOMURA, AKIKO

× TONOMURA, AKIKO

WEKO 27563

TONOMURA, AKIKO

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OHARA, TAKAYUKI

× OHARA, TAKAYUKI

WEKO 27564

OHARA, TAKAYUKI

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ITAYA, TOSHIMITSU

× ITAYA, TOSHIMITSU

WEKO 27565

ITAYA, TOSHIMITSU

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KAGAMI, HIDEAKI

× KAGAMI, HIDEAKI

WEKO 27566

KAGAMI, HIDEAKI

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UEDA, MINORU

× UEDA, MINORU

WEKO 27567

UEDA, MINORU

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キーワード
主題Scheme Other
主題 Dentin bridge
キーワード
主題Scheme Other
主題 Dental pulp cells
キーワード
主題Scheme Other
主題 Hydroxyapatite/b-TCP
キーワード
主題Scheme Other
主題 Tissue engineering
キーワード
主題Scheme Other
主題 Pulp capping
抄録
内容記述 The purpose of this study was to investigate dentin-bridge formation in teeth following the transplantation of dental pulp-derived cells seeded on hydroxyapatite/tricalcium phosphate (HA/TCP) scaffolds. The dental pulp tissues were removed from the extracted first molar teeth of miniature pigs and single cell populations were subcultured. Second-passage cells that had alkaline phosphatase activity were combined with scaffolds. Cell-scaffold constructs were placed in contact with the exposed pulp tissue. The dimensions of the exposed pulp site were approximately 1–2.5 mm in diameter and 2–3 mm in depth from the tooth surface. After placing the constructs, the tooth was restored with composite resin. Six weeks after transplantation, hard tissue formation was observed on the pulp tissue in histology. Dentinal tubule-like structures were observed in most of the hard tissue generated, and columnar cells, which showed positive immunoreactions with dentin sialoprotein (DSP) and heat shock protein (HSP)-25, were aligned beneath the hard tissues. When only scaffolds were placed on the pulp tissues, particles of hard tissue were formed, however dentinal tubule-like structures and odontoblasts were not observed despite the formation of hard tissue. In conclusion, the implantation of dental pulp constructs into pulp exposed stimulates the formation of calcified dentin-like structures.
内容記述タイプ Abstract
出版者
出版者 Nagoya University School of Medicine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/nagjms.71.1-2.51
ID登録タイプ JaLC
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 0027-7622
書誌情報 Nagoya Journal of Medical Science

巻 71, 号 1-2, p. 51-62, 発行日 2009-02
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/11336
識別子タイプ HDL
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