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THE INDUCTION OF DENTIN BRIDGE-LIKE STRUCTURES BY CONSTRUCTS OF SUBCULTURED DENTAL PULP-DERIVED CELLS AND POROUS HA/TCP IN PORCINE TEETH
https://doi.org/10.18999/nagjms.71.1-2.51
https://doi.org/10.18999/nagjms.71.1-2.51faa75f31-a118-458b-a8a7-316953aea005
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2009-03-19 | |||||
タイトル | ||||||
タイトル | THE INDUCTION OF DENTIN BRIDGE-LIKE STRUCTURES BY CONSTRUCTS OF SUBCULTURED DENTAL PULP-DERIVED CELLS AND POROUS HA/TCP IN PORCINE TEETH | |||||
著者 |
ANDO, YUSUKE
× ANDO, YUSUKE× HONDA, MASAKI J.× OHSHIMA, HAYATO× TONOMURA, AKIKO× OHARA, TAKAYUKI× ITAYA, TOSHIMITSU× KAGAMI, HIDEAKI× UEDA, MINORU |
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キーワード | ||||||
主題Scheme | Other | |||||
主題 | Dentin bridge | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Dental pulp cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hydroxyapatite/b-TCP | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tissue engineering | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pulp capping | |||||
抄録 | ||||||
内容記述 | The purpose of this study was to investigate dentin-bridge formation in teeth following the transplantation of dental pulp-derived cells seeded on hydroxyapatite/tricalcium phosphate (HA/TCP) scaffolds. The dental pulp tissues were removed from the extracted first molar teeth of miniature pigs and single cell populations were subcultured. Second-passage cells that had alkaline phosphatase activity were combined with scaffolds. Cell-scaffold constructs were placed in contact with the exposed pulp tissue. The dimensions of the exposed pulp site were approximately 1–2.5 mm in diameter and 2–3 mm in depth from the tooth surface. After placing the constructs, the tooth was restored with composite resin. Six weeks after transplantation, hard tissue formation was observed on the pulp tissue in histology. Dentinal tubule-like structures were observed in most of the hard tissue generated, and columnar cells, which showed positive immunoreactions with dentin sialoprotein (DSP) and heat shock protein (HSP)-25, were aligned beneath the hard tissues. When only scaffolds were placed on the pulp tissues, particles of hard tissue were formed, however dentinal tubule-like structures and odontoblasts were not observed despite the formation of hard tissue. In conclusion, the implantation of dental pulp constructs into pulp exposed stimulates the formation of calcified dentin-like structures. | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
出版者 | Nagoya University School of Medicine | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.18999/nagjms.71.1-2.51 | |||||
ID登録タイプ | JaLC | |||||
ISSN(print) | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0027-7622 | |||||
書誌情報 |
Nagoya Journal of Medical Science 巻 71, 号 1-2, p. 51-62, 発行日 2009-02 |
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フォーマット | ||||||
application/pdf | ||||||
著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/11336 | |||||
識別子タイプ | HDL |