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The molar fossil was cleaned on the surface with a steel brush and a dental grinder, treated with 0.5M NaOH solution, and ground into powder. Two sets of the powdered sample, about 3.8g and 4.9g each, were demineralized with 1.2N HCl in a cellulose tube to extract collagen. After demineralization, HCl was completely removed from the tube by dialysis. Then the solution fraction in the tube was separated by centrifuge, and freeze-dried to get a solution-collagen fraction. Solid remains were put in a Pyrex tube with 20ml of distilled water and heated at 90℃ for 10 hours to extract the water soluble gelatin-collagen fraction. Collagen was oxidized, using CuO to produce CO_2,in a Vycor tube at 950℃ for an hour. A small aliquot of the CO_2 was analyzed for its\u0027 stable carbon isotope ratio, δ ^\u003c13\u003eC_\u003cPDB\u003e, using a triple-collector mass spectrometer (MAT-252). To produce graphite, the remaining CO_2 was reduced by hydrogen, with an Fe-powder catalyst, in a Vycor tube at 650℃ for several hours. The sample graphite was analyzed for its\u0027 ^\u003c14\u003eC/^\u003c13\u003eC ratio, relative to that of an oxalic acid standard (NBS-SRM-4990). In general, reliable ^\u003c14\u003eC dates can be obtained for collagen fractions extracted from the fossil sample, provided that the collagen fractions have been preserved well from weathering in nature. Experimental yields of collagen fractions were relatively high, ranging from 1.22 to 1.51%. The CO_2 yield from the gelatin collagen fraction was 42%, being consistent with the values of 41-42% for gelatin collagen extracted from fresh bone samples. On the other hand, the CO_2 yield from the solution fraction was 25%, being lower than the values of 41-42%. 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  1. F300 宇宙地球環境研究所
  2. F300b 紀要
  3. 名古屋大学加速器質量分析計業績報告書
  4. 9

愛媛県西宇和郡三崎町名取梶谷鼻沖で採取されたナウマン象の臼歯のAMS ^<14>C年代

https://doi.org/10.18999/10.19999/sumrua.9.286
https://doi.org/10.18999/10.19999/sumrua.9.286
ff767c25-8218-48bd-aa55-b2528de42073
名前 / ファイル ライセンス アクション
KJ00002410076.pdf KJ00002410076.pdf (840.4 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2010-05-21
タイトル
タイトル 愛媛県西宇和郡三崎町名取梶谷鼻沖で採取されたナウマン象の臼歯のAMS ^<14>C年代
その他のタイトル
その他のタイトル AMS ^<14>C Age of Collagen Separated from a Molar Fossil of Naumann's Elephant Collected from the Uwa-sea, Ehime Prefecture
著者 中村, 俊夫

× 中村, 俊夫

WEKO 80660

中村, 俊夫

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Nakamura, Toshio

× Nakamura, Toshio

WEKO 80661

Nakamura, Toshio

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太田, 友子

× 太田, 友子

WEKO 80662

太田, 友子

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Ohta, Tomoko

× Ohta, Tomoko

WEKO 80663

Ohta, Tomoko

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宮本, 雅三

× 宮本, 雅三

WEKO 80664

宮本, 雅三

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Miyamoto, Masazo

× Miyamoto, Masazo

WEKO 80665

Miyamoto, Masazo

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南, 雅代

× 南, 雅代

WEKO 80666

南, 雅代

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Minami, Masayo

× Minami, Masayo

WEKO 80667

Minami, Masayo

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小田, 寛貴

× 小田, 寛貴

WEKO 80668

小田, 寛貴

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Oda, Hirotaka

× Oda, Hirotaka

WEKO 80669

Oda, Hirotaka

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池田, 晃子

× 池田, 晃子

WEKO 80670

池田, 晃子

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Ikeda, Akiko

× Ikeda, Akiko

WEKO 80671

Ikeda, Akiko

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権利
権利情報 名古屋大学年代測定資料研究センター 天然放射性元素測定小委員会
権利
権利情報 本文データは学協会の許諾に基づきCiNiiから複製したものである
キーワード
主題Scheme Other
主題 哺乳類化石
キーワード
主題Scheme Other
主題 ナウマン象
キーワード
主題Scheme Other
主題 臼歯化石
キーワード
主題Scheme Other
主題 コラーゲン
キーワード
主題Scheme Other
主題 加速器質量分析
キーワード
主題Scheme Other
主題 ^<14>C年代
抄録
内容記述 We have conducted ^<14>C dating, with a Tandetron accelerator mass spectrometer (AMS) at Nagoya University, on carbon from collagen fractions extracted from a molar tooth fossil of Naumann's elephant (Palaeoloxodon naumanni) collected from the Uwa-sea, offshore Natori-Kajitanihana, Nishi-Uwa-gun, Ehime prefecture. The molar fossil was cleaned on the surface with a steel brush and a dental grinder, treated with 0.5M NaOH solution, and ground into powder. Two sets of the powdered sample, about 3.8g and 4.9g each, were demineralized with 1.2N HCl in a cellulose tube to extract collagen. After demineralization, HCl was completely removed from the tube by dialysis. Then the solution fraction in the tube was separated by centrifuge, and freeze-dried to get a solution-collagen fraction. Solid remains were put in a Pyrex tube with 20ml of distilled water and heated at 90℃ for 10 hours to extract the water soluble gelatin-collagen fraction. Collagen was oxidized, using CuO to produce CO_2,in a Vycor tube at 950℃ for an hour. A small aliquot of the CO_2 was analyzed for its' stable carbon isotope ratio, δ ^<13>C_<PDB>, using a triple-collector mass spectrometer (MAT-252). To produce graphite, the remaining CO_2 was reduced by hydrogen, with an Fe-powder catalyst, in a Vycor tube at 650℃ for several hours. The sample graphite was analyzed for its' ^<14>C/^<13>C ratio, relative to that of an oxalic acid standard (NBS-SRM-4990). In general, reliable ^<14>C dates can be obtained for collagen fractions extracted from the fossil sample, provided that the collagen fractions have been preserved well from weathering in nature. Experimental yields of collagen fractions were relatively high, ranging from 1.22 to 1.51%. The CO_2 yield from the gelatin collagen fraction was 42%, being consistent with the values of 41-42% for gelatin collagen extracted from fresh bone samples. On the other hand, the CO_2 yield from the solution fraction was 25%, being lower than the values of 41-42%. Thus the ^<14>C date of 29,200±870 yr BP, obtained for the gelatin collagen fraction, seems to be reliable and can be assigned as the age of the molar fossil sample, though the ^<14>C date is about 4,000 years older than the value of 24,880±580 yr BP obtained for the solution collagen fraction. The δ ^<13>C_<PDB> values of -20.2‰ for the both gelatin and solution collagen fractions were consistent with accepted δ ^<13>C_<PDB> values, of from -21 to -23‰ for herbivorous mammals.
内容記述タイプ Abstract
内容記述
内容記述 タンデトロン加速器質量分析計業績報告 Summaries of Researches Using AMS 1997 (平成9)年度
内容記述タイプ Other
出版者
出版者 名古屋大学年代測定資料研究センター 天然放射性元素測定小委員会
言語
言語 jpn
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/10.19999/sumrua.9.286
ID登録タイプ JaLC
関連情報
関連タイプ isVersionOf
識別子タイプ URI
関連識別子 http://ci.nii.ac.jp/naid/110001136070/
書誌情報 名古屋大学加速器質量分析計業績報告書

巻 9, p. 286-297, 発行日 1998-03
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