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Body color mutants and a transposable element affecting the tyrosinase gene of the medaka fish, Oryzias latipes
https://doi.org/10.18999/fisbjm.6.7
https://doi.org/10.18999/fisbjm.6.78c312d5f-f58e-462f-bf4d-d02fdb3eb957
名前 / ファイル | ライセンス | アクション |
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KJ00005673862.pdf (713.1 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2010-06-17 | |||||
タイトル | ||||||
タイトル | Body color mutants and a transposable element affecting the tyrosinase gene of the medaka fish, Oryzias latipes | |||||
言語 | en | |||||
著者 |
Koga, Akihiko
× Koga, Akihiko× Inagaki, Hidehito× Bessho, Yoshitaka× Hori, Hirosi |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | Laboratory of Freshwater Fish Stocks Bioscience Center Nagoya University | |||||
権利 | ||||||
言語 | ja | |||||
権利情報 | 本文データは学協会の許諾に基づきCiNiiから複製したものである | |||||
抄録 | ||||||
内容記述 | In the medaka fish Oryzias latipes, many mutants for body colors have been isolated. Among them, albinos (i-locus alleles) are typical color mutants having amelanotic skin and red-colored eyes with no tyrosinase activity. To cast light on the molecular basis of the albino mechanism, we have cloned cDNAs for tyrosinase, a key enzyme in melanin biosynthesis, from the wild-type fish, and performed Southern blot analysis on genomic DNA from an i mutant fish with the cDNA probe. The results demonstrated a 1.9-kb insertion fragment in exon 1 of the tyrosinase gene. The fragment, designated To1, (i) carries terminal inverted repeats containing sequences quite similar to those of the Tc1 transposable element, (ii) is flanked by duplicated segments of their host chromosomes, and (iii) is present in approximately 80 copies in the genome, showing specific features of the Tc1-like transposable element. It is clear that insertion of To1 into the tyrosinase gene causes the albino body color in the i mutant. As a contrast to albino-i alleles, there is a colorless melanophore mutant b, having normally pigmented black eyes but orange-colored skin with amelanotic melanophores, thus showing the presence of a tissue-specific mechanism of melanin formation. Using the cDNA probe, Northern blot analysis on poly(A)^+RNAs from various tissues was carried out. In the wild-type, the tyrosinase mRNA was expressed in eyes and skin but not in liver, corresponding to tissue-specific melanin formation. In the b mutant, contrary to expectation, the mRNA was detected not only in eyes but also in amelanotic skin. Therefore, pigmentation of skin controlled by b is not directly related to expression of the tyrosinase gene. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Laboratory of Freshwater Fish Stocks Bioscience Center Nagoya University | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
ID登録 | ||||||
ID登録 | 10.18999/fisbjm.6.7 | |||||
ID登録タイプ | JaLC | |||||
関連情報 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | URI | |||||
関連識別子 | http://ci.nii.ac.jp/naid/110007349010/ | |||||
書誌情報 |
en : The Fish Biology Journal Medaka 巻 6, p. 7-15, 発行日 1994 |
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フォーマット | ||||||
application/pdf | ||||||
著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/13788 | |||||
識別子タイプ | HDL |