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  1. B100 理学部/理学研究科
  2. B100a 雑誌掲載論文
  3. 学術雑誌

Manipulating behaviors of targeted single cells in vivo by using IR-LEGO

http://hdl.handle.net/2237/13889
a01a9ab5-e4bb-441d-bf93-0ac7e3766847
名前 / ファイル ライセンス アクション
Takagi_Shin.pdf Takagi_Shin.pdf (163.2 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2010-07-30
タイトル
タイトル Manipulating behaviors of targeted single cells in vivo by using IR-LEGO
著者 Suzuki, Motoshi

× Suzuki, Motoshi

WEKO 38073

Suzuki, Motoshi

Search repository
Kamei, Yasuhiro

× Kamei, Yasuhiro

WEKO 38074

Kamei, Yasuhiro

Search repository
Yuba, Shunsuke

× Yuba, Shunsuke

WEKO 38075

Yuba, Shunsuke

Search repository
Takagi, Shin

× Takagi, Shin

WEKO 38076

Takagi, Shin

Search repository
権利
権利情報 ©2009 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE.
抄録
内容記述 Methods for turning on/off gene expression at any desired time and place in vivo would be useful for analyzing various biological processes. We have developed a novel microscopic system utilizing an infrared laser, IR-LEGO (infrared-laser evoked gene operator), which is designed to deposit heat locally in living organisms. We have shown that IR-LEGO enables us to induce the heat shock response efficiently in targeted single cells of C. elegans worms, thereby driving expression of a transgene under the control of a heat shock promoter. By using IR-LEGO we attempted to rescue several mutant phenotypes of worms at the single-cell level. Diverse cell behaviors including differentiation and migration of target cells can be manipulated by gene induction mediated by IR-LEGO. Our results showed that IR-LEGO can be used to manipulate cell-autonomous as well as cell-nonautonomous behaviors, further confirming that irradiation using IR-LEGO has no harmful effects on the targets. Thus, IR-LEGO serves as valuable tools for manipulating biological processes in living organisms.
内容記述タイプ Abstract
出版者
出版者 IEEE
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
ISBN
関連識別子
識別子タイプ ISBN
関連識別子 978-1-4244-5094-7
書誌情報 International Symposium on Micro-NanoMechatronics and Human Science : MHS2009

p. 326-331, 発行日 2009-09-11
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/13889
識別子タイプ HDL
URI
識別子 http://dx.doi.org/10.1109/MHS.2009.5351899
識別子タイプ DOI
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