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  1. B200 工学部/工学研究科
  2. B200a 雑誌掲載論文
  3. 学術雑誌

Optical pH Regulation Using Functional Nanotool Impregnating with Photo-Responsive Chemical for Intracellular Measurement

http://hdl.handle.net/2237/14484
04e9e562-69ec-4ebd-bcaa-a7e95317baa7
名前 / ファイル ライセンス アクション
1086.pdf 1086.pdf (1.5 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2011-02-16
タイトル
タイトル Optical pH Regulation Using Functional Nanotool Impregnating with Photo-Responsive Chemical for Intracellular Measurement
著者 Maruyama, Hisataka

× Maruyama, Hisataka

WEKO 39640

Maruyama, Hisataka

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Masuda, Taisuke

× Masuda, Taisuke

WEKO 39641

Masuda, Taisuke

Search repository
Inoue, Naoya

× Inoue, Naoya

WEKO 39642

Inoue, Naoya

Search repository
Honda, Ayae

× Honda, Ayae

WEKO 39643

Honda, Ayae

Search repository
Takahata, Tatsuro

× Takahata, Tatsuro

WEKO 39644

Takahata, Tatsuro

Search repository
Arai, Fumihito

× Arai, Fumihito

WEKO 39645

Arai, Fumihito

Search repository
抄録
内容記述タイプ Abstract
内容記述 We developed Optical pH regulation using functional nanotool impregnated with photo-responsive chemical for selective cell injection of nanotool. The nanotool was modified by fluorescent dye for intracellular measurement. The nanotool was included in the fusogenic liposome. Membrane fusion of the liposome to the cell membrane was used for invasive cell injection of the nanotool. The liposome fuses to the cell in weak acidic condition. Local pH regulation inside the liposome was developed using photochromic chemical for selective cell injection of the nanotool. The nanotool was modified by Leuco crystal violet (LCV). LCV emits the proton by ultraviolet (UV) illumination. The emitted proton decreases the pH value in the liposome. This pH regulation is reversible by UV/VIS illumination. The liposome was manipulated by optical tweezers. After contact of the liposome to the cell, the liposome was adhered to the cell by UV induced membrane fusion. Injected nanotool was manipulated by optical tweezers. Intracellular temperature was detected by measuring the fluorescence intensity of the nanosensor. We demonstrated optical pH regulation, selective cell injection of the nanotool, and manipulation of the nanotool in the cell.
出版者
出版者 IEEE
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
ISBN
関連識別子
識別子タイプ ISBN
関連識別子 978-1-4244-7995-5
書誌情報 International Symposium on Micro-NanoMechatronics and Human Science (MHS)

p. 162-167, 発行日 2010
著者版フラグ
値 author
URI
識別子 http://hdl.handle.net/2237/14484
識別子タイプ HDL
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