{"_buckets": {"deposit": "e70181f2-026b-4e90-836e-dd4492747b9a"}, "_deposit": {"id": "12944", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "12944"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00012944", "sets": ["961"]}, "author_link": ["40803"], "item_12_alternative_title_19": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "ヒト乳癌細胞株MCF-7 における抗癌剤ダウノルビシン による中性スフィンゴミエリナー2 遺伝子発現の転写調節機序", "subitem_alternative_title_language": "ja"}]}, "item_12_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2011-03-25", "bibliographicIssueDateType": "Issued"}}]}, "item_12_date_granted_64": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2011-03-25"}]}, "item_12_degree_grantor_62": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_language": "ja", "subitem_degreegrantor_name": "名古屋大学"}, {"subitem_degreegrantor_language": "en", "subitem_degreegrantor_name": "NAGOYA University"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "13901", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_12_degree_name_61": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士(医療技術学)", "subitem_degreename_language": "ja"}]}, "item_12_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Mg^2+-dependent neutral SMases (NSMases) have emerged as prime candidates for stress-induced ceramide production. Among isoforms identified, previous reports have suggested the importance of NSMase2. However, its activation mechanism has not been precisely reported. Here, we analyzed the mechanism of NSMase2 gene expression by the anti-cancer drug, daunorubicin (DA). DA increased cellular ceramides (C16, C18 and C24) and NSMase activity of a human breast cancer cell line, MCF-7. DA remarkably increased the NSMase2 message and protein, whereas little change in NSMase1 and NSMase3 mRNA and only a mild increase in acid SMase mRNA were observed. Overexpression and a knock down of NSMase2 indicated that NSMase2 played a role in DA-induced cell death. NSMase2 promoter analysis revealed that three Sp1 motifs located between -148~-42 bp upstream of the first exon were important in basic as well as in DA-induced promoter activity. Consistently, luciferase vectors containing three consensus Sp1-motifs but not its mutated form showed DA-induced transcriptional activation. DA-treated MCF-7 showed increased Sp3 protein. In SL2 cells lacking Sp family proteins, both Sp1 and Sp3 overexpression increased NSMase promoter activity. Increased binding of Sp family proteins by DA to three Sp1 motifs was shown by electrophoresis mobility-shift and ChIP assays.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_12_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "名古屋大学博士学位論文 学位の種類:博士(医療技術学) (課程) 学位授与年月日:平成23年3月25日", "subitem_description_language": "ja", "subitem_description_type": "Other"}]}, "item_12_dissertation_number_65": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "甲第9069号"}]}, "item_12_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/14839"}]}, "item_12_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_12_text_63": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "2010"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "伊藤, 裕美", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "40803", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "k9069.pdf", "filesize": [{"value": "1.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 1300000.0, "url": {"label": "k9069.pdf", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/12944/files/k9069.pdf"}, "version_id": "15c34292-31da-4b11-81cb-9cf730804997"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "NSMase2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Daunorubicin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ceramide", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Promoter analysis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "EMSA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ChIP", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Sp family protein", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Transcriptional regulation of neutral sphingomyelinase 2 gene expression of a human breast cancer cell line, MCF-7, induced by the anti-cancer drug, daunorubicin", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Transcriptional regulation of neutral sphingomyelinase 2 gene expression of a human breast cancer cell line, MCF-7, induced by the anti-cancer drug, daunorubicin", "subitem_title_language": "en"}]}, "item_type_id": "12", "owner": "1", "path": ["961"], "permalink_uri": "http://hdl.handle.net/2237/14839", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2011-05-20"}, "publish_date": "2011-05-20", "publish_status": "0", "recid": "12944", "relation": {}, "relation_version_is_last": true, "title": ["Transcriptional regulation of neutral sphingomyelinase 2 gene expression of a human breast cancer cell line, MCF-7, induced by the anti-cancer drug, daunorubicin"], "weko_shared_id": -1}