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N-Acetylglucosamine 6-O-Sulfotransferase-1-Deficient Mice Show Better Functional Recovery after Spinal Cord Injury
http://hdl.handle.net/2237/15077
http://hdl.handle.net/2237/150773bf1885d-81bd-4c21-bc09-56e6b32afbdb
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
k8972.pdf (3.3 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2011-07-19 | |||||
| タイトル | ||||||
| タイトル | N-Acetylglucosamine 6-O-Sulfotransferase-1-Deficient Mice Show Better Functional Recovery after Spinal Cord Injury | |||||
| 言語 | en | |||||
| 著者 |
ITO, Zenya
× ITO, Zenya× Sakamoto, Kazuma× Imagama, Shiro× Matsuyama, Yukihiro× Zhang, Haoqian× Hirano, Kenichi× Ando, Kei× Yamashita, Toshihide× Ishiguro, Naoki× Kadomatsu, Kenji |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Neurons in the adult CNS do not spontaneously regenerate after injuries. The glycosaminoglycan keratan sulfate is induced after spinal cord injury, but its biological significance is not well understood. Here we investigated the role of keratan sulfate in functional recovery after spinal cord injury, using mice deficient in N-acetylglucosamine 6-O-sulfotransferase-1 that lack 5D4-reactive keratan sulfate in the CNS. We made contusion injuries at the 10th thoracic level. Expressions of N-acetylglucosamine 6-O-sulfotransferase-1 and keratan sulfate were induced after injury in wild-type mice, but not in the deficient mice. The wild-type and deficient mice showed similar degrees of chondroitin sulfate induction and of CD11b-positive inflammatory cell recruitment. However, motor function recovery, as assessed by the footfall test, footprint test, and Basso mouse scale locomotor scoring, was significantly better in the deficient mice. Moreover, the deficient mice showed a restoration of neuromuscular system function below the lesion after electrical stimulation at the occipito-cervical area. In addition, axonal regrowth of both the corticospinal and raphespinal tracts was promoted in the deficient mice. In vitro assays using primary cerebellar granule neurons demonstrated that keratan sulfate proteoglycans were required for the proteoglycan-mediated inhibition of neurite outgrowth. These data collectively indicate that keratan sulfate expression is closely associated with functional disturbance after spinal cord injury.N-acetylglucosamine 6-O-sulfotransferase-1-deficient mice are a good model to investigate the roles of keratan sulfate in the CNS. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 名古屋大学博士学位論文 学位の種類 : 博士(医学)(課程) 学位授与年月日:平成22年10月29日 伊藤全哉氏の博士論文として提出された | |||||
| 言語 | ja | |||||
| 出版者 | ||||||
| 出版者 | Society for Neuroscience | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_db06 | |||||
| タイプ | doctoral thesis | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1523/JNEUROSCI.2570-09.2010 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0270-6474 | |||||
| 書誌情報 |
en : The Journal of Neuroscience 巻 30, 号 17, p. 5937-5947, 発行日 2010-04-28 |
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| 学位名 | ||||||
| 言語 | ja | |||||
| 学位名 | 博士(医学) | |||||
| 学位授与機関 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 13901 | |||||
| 言語 | ja | |||||
| 学位授与機関名 | 名古屋大学 | |||||
| 言語 | en | |||||
| 学位授与機関名 | NAGOYA University | |||||
| 学位授与年度 | ||||||
| 学位授与年度 | 2010 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2010-04-28 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲第8972号 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
| URI | ||||||
| 識別子 | http://dx.doi.org/10.1523/JNEUROSCI.2570-09.2010 | |||||
| 識別子タイプ | DOI | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/15077 | |||||
| 識別子タイプ | HDL | |||||
