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  1. F100 環境医学研究所
  2. F100b 紀要
  3. Environmental medicine : annual report of the Research Institute of Environmental Medicine
  4. 46(1-2)

Cloning of a cDNA for Type II Iodothyronine 5' Deiodinase in the House Musk Shrew (Suncus murinus. Insectivora : Soricidae)

https://doi.org/10.18999/envm.46.62
https://doi.org/10.18999/envm.46.62
1ea1aa5c-65b9-4d0b-b538-62697cd014cd
名前 / ファイル ライセンス アクション
KJ00000034865.pdf KJ00000034865.pdf (390.5 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2006-01-05
タイトル
タイトル Cloning of a cDNA for Type II Iodothyronine 5' Deiodinase in the House Musk Shrew (Suncus murinus. Insectivora : Soricidae)
言語 en
著者 SUZUKI, Daisuke

× SUZUKI, Daisuke

WEKO 3142

en SUZUKI, Daisuke

Search repository
AKEUCHI, Yoko

× AKEUCHI, Yoko

WEKO 3143

en AKEUCHI, Yoko

Search repository
ODA, Sen-ichi

× ODA, Sen-ichi

WEKO 3144

en ODA, Sen-ichi

Search repository
MURATA, Yoshiharu

× MURATA, Yoshiharu

WEKO 3145

en MURATA, Yoshiharu

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
キーワード
主題Scheme Other
主題 Type II iodothyronine deiodinase
キーワード
主題Scheme Other
主題 house musk shrew
キーワード
主題Scheme Other
主題 cloning
キーワード
主題Scheme Other
主題 RACE
キーワード
主題Scheme Other
主題 selenocystein
抄録
内容記述 Two types of iodothyronine 5' deiodinase are known to catalyze the conversion of thyroxine to 3,3',5-triiodothyroine (T3), an active form of thyroid hormone. Our recent study has suggested that type II deiodinase rather than type I is important for the maintenance of plasma T3 as well as the tissue content of T3 in the house musk shrew. The present study aimed to clone cDNA for type II deiodinase and determine the primary structure of DII protein. Total RNA was extracted from the brown adipose tissue in the shrew of Katmandu strain (KAT), where highest DII activity was detected. DII cDNA was cloned in combination by RT-PCR and Rapid Amplification of cDNA Ends (RACE). The alignment of three cloned cDNA fragments includes the entire open reading frame of shrew DII so that the amino acid sequence was deduced. The shrew DII contains two selenocysteins that are essential for the deiodinase activity. The amino acid sequence of the shrew DII showed high homology with that of human (89%), rat (91 %) and mouse (92%). The obtained cDNA would be a proper probe to investigate changes in the DII expression when shrews are exposed to various environments and insults.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 国立情報学研究所で電子化したコンテンツを使用している。
言語 ja
内容記述タイプ Other
出版者
言語 en
出版者 Research Institute of Environmental Medicine, Nagoya University
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
ID登録
ID登録 10.18999/envm.46.62
ID登録タイプ JaLC
ISSN(print)
収録物識別子タイプ PISSN
収録物識別子 02870517
書誌情報 en : Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University

巻 46, 号 1/2, p. 62-65, 発行日 2002-12
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/2785
識別子タイプ HDL
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