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Cloning of a cDNA for Type II Iodothyronine 5' Deiodinase in the House Musk Shrew (Suncus murinus. Insectivora : Soricidae)
https://doi.org/10.18999/envm.46.62
https://doi.org/10.18999/envm.46.621ea1aa5c-65b9-4d0b-b538-62697cd014cd
名前 / ファイル | ライセンス | アクション |
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KJ00000034865.pdf (390.5 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2006-01-05 | |||||
タイトル | ||||||
タイトル | Cloning of a cDNA for Type II Iodothyronine 5' Deiodinase in the House Musk Shrew (Suncus murinus. Insectivora : Soricidae) | |||||
言語 | en | |||||
著者 |
SUZUKI, Daisuke
× SUZUKI, Daisuke× AKEUCHI, Yoko× ODA, Sen-ichi× MURATA, Yoshiharu |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Type II iodothyronine deiodinase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | house musk shrew | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cloning | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | RACE | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | selenocystein | |||||
抄録 | ||||||
内容記述 | Two types of iodothyronine 5' deiodinase are known to catalyze the conversion of thyroxine to 3,3',5-triiodothyroine (T3), an active form of thyroid hormone. Our recent study has suggested that type II deiodinase rather than type I is important for the maintenance of plasma T3 as well as the tissue content of T3 in the house musk shrew. The present study aimed to clone cDNA for type II deiodinase and determine the primary structure of DII protein. Total RNA was extracted from the brown adipose tissue in the shrew of Katmandu strain (KAT), where highest DII activity was detected. DII cDNA was cloned in combination by RT-PCR and Rapid Amplification of cDNA Ends (RACE). The alignment of three cloned cDNA fragments includes the entire open reading frame of shrew DII so that the amino acid sequence was deduced. The shrew DII contains two selenocysteins that are essential for the deiodinase activity. The amino acid sequence of the shrew DII showed high homology with that of human (89%), rat (91 %) and mouse (92%). The obtained cDNA would be a proper probe to investigate changes in the DII expression when shrews are exposed to various environments and insults. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
内容記述 | ||||||
内容記述 | 国立情報学研究所で電子化したコンテンツを使用している。 | |||||
言語 | ja | |||||
内容記述タイプ | Other | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Research Institute of Environmental Medicine, Nagoya University | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
ID登録 | ||||||
ID登録 | 10.18999/envm.46.62 | |||||
ID登録タイプ | JaLC | |||||
ISSN(print) | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 02870517 | |||||
書誌情報 |
en : Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University 巻 46, 号 1/2, p. 62-65, 発行日 2002-12 |
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フォーマット | ||||||
application/pdf | ||||||
著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/2785 | |||||
識別子タイプ | HDL |