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TRANSEPITHELIAL MOVEMENT OF 3H-ANDROGEN IN RAT SEMINIFEROUS AND CAPUT EPIDIDYMAL TUBULES: SATURABILITY AND EFFECT OF COMPETITION WITH ESTRADIOL
https://doi.org/10.18999/nagjms.56.1-4.81
https://doi.org/10.18999/nagjms.56.1-4.817ef41c7f-eea7-47f1-b933-1e0eba42b430
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2012-02-07 | |||||
タイトル | ||||||
タイトル | TRANSEPITHELIAL MOVEMENT OF 3H-ANDROGEN IN RAT SEMINIFEROUS AND CAPUT EPIDIDYMAL TUBULES: SATURABILITY AND EFFECT OF COMPETITION WITH ESTRADIOL | |||||
著者 |
YAMAMOTO, MASANORI
× YAMAMOTO, MASANORI× TSUJI, YOSHIKAZU× MIYAKE, KOJI |
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キーワード | ||||||
主題Scheme | Other | |||||
主題 | Androgen movement | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | epididymis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | estradiol | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | microperifusion | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | micropuncture | |||||
抄録 | ||||||
内容記述 | Proluminal movement of 3H-testosterone from peritubular space to intratubular fluids of the adult rat testis and epididymis was studied by using in vivo microperifusion and subsequent micropuncture of seminiferous tubules and caput epididymal tubules. Tubules were perifused with Minimum Essential Medium containing 3H-testosterone. To determine if androgen transport is saturable, 40, 80, 160, and 320 μCi 3H-testosterone were included in the perifusion fluid. Radioactivity of 3H-androgens in the intraluminal fluids was determined at 1 hour after perifusion. Transepithelial 3H-androgen movement in the testis was linear (r=0.735, p<O.OOl, y=0.17x+5.6). The movement of 3H-androgen across the epididymal epithelium was saturable (Vmax of 326.5 nM/hr and Km of 77 nM). To determine the effect of estradiol on proluminal androgen movement, estradiol at 10X the cocentration of 3H-testosterone was incorporated in the perifusion fluid. Proluminal 3H-androgen movement into the seminiferous and epididymal tubules was not affected by addition of estradiol to the perifusion fluid. These findings support our previous observations that proluminal transepithelial movement of 3H-androgens could be mediated by its binding to a specific intraluminal androgen binding protein. | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
出版者 | Nagoya University School of Medicine | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.18999/nagjms.56.1-4.81 | |||||
ID登録タイプ | JaLC | |||||
関連情報 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | URI | |||||
関連識別子 | https://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5614/5614.html | |||||
ISSN(print) | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0027-7622 | |||||
ISSN(Online) | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2186-3326 | |||||
書誌情報 |
Nagoya Journal of Medical Science 巻 56, 号 1-4, p. 81-87, 発行日 1993-11-25 |
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著者版フラグ | ||||||
値 | publisher |