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ESTABLISHMENT AND OPTIMAL CULTURE CONDITIONS OF MICRORNA-INDUCED PLURIPOTENT STEM CELLS GENERATED FROM HEK293 CELLS VIA TRANSFECTION OF MICRORNA-302S EXPRESSION VECTOR
https://doi.org/10.18999/nagjms.74.1-2.157
https://doi.org/10.18999/nagjms.74.1-2.157809dff97-28f8-4bcf-879d-16a47341ac38
名前 / ファイル | ライセンス | アクション |
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16_Koide.pdf (16.0 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2012-03-01 | |||||
タイトル | ||||||
タイトル | ESTABLISHMENT AND OPTIMAL CULTURE CONDITIONS OF MICRORNA-INDUCED PLURIPOTENT STEM CELLS GENERATED FROM HEK293 CELLS VIA TRANSFECTION OF MICRORNA-302S EXPRESSION VECTOR | |||||
言語 | en | |||||
著者 |
KOIDE, NAOSHI
× KOIDE, NAOSHI× YASUDA, KAORI× KADOMATSU, KENJI× TAKEI, YOSHIFUMI |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Embryonic stem cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pluripotent stem cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Reprogramming | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | MicroRNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Regenerative medicine | |||||
抄録 | ||||||
内容記述 | Induced pluripotent stem cells (iPSCs) have been directly generated from fibroblast cultures though retrovirus- or lentivirus-mediated ectopic overexpression of only a few defined transcriptional factors. This remarkable achievement has greatly enhanced our ability to explore the causes of, and potential cures for, many genetic diseases, and strengthened the promise of regenerative medicine. In fact, to date, many kinds of somatic cells from different tissues have exhibited a capacity for reprogramming toward an embryonic stem cell-like state, but major bottlenecks in iPSC derivation and therapeutic use remain, including low reprogramming efficiencies and the tumorigenesis of the generated iPSC. Here, we successfully generated miR-302s-induced pluripotent stem cells (mirPS cells) from human embryonic kidney (HEK) 293 cells via transfection of the miR-302s expression vector. We also determined the optimal culture conditions to generate mirPS on feeder cells, which included the use of serum-free N2B27 medium. The mirPS cells generated by our improved conditions showed the expression of pluripotent marker genes such as OCT3/4, NANOG, and SOX2 under growth conditions via reverse transcription-PCR, whereas no expression of these genes was observed in HEK293 cells. On the other hand, under differentiation conditions, mirPS cells formed ball-shaped structures (embryoid bodies), and showed the ability to differentiate into three germ layers (ectoderm, mesoderm, and endoderm) in vitro. The results suggested that our generated mirPS cells are actually functional as a cell resource to apply to regenerative medicine, and mirPS cells are suitable materials to clarify the mechanism underlying the reprogramming from somatic cells. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Nagoya University School of Medicine | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
ID登録 | ||||||
ID登録 | 10.18999/nagjms.74.1-2.157 | |||||
ID登録タイプ | JaLC | |||||
関連情報 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | URI | |||||
関連識別子 | http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/7412/7412.html | |||||
ISSN(print) | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0027-7622 | |||||
ISSN(Online) | ||||||
収録物識別子タイプ | EISSN | |||||
収録物識別子 | 2186-3326 | |||||
書誌情報 |
en : Nagoya Journal of Medical Science 巻 74, 号 1-2, p. 157-165, 発行日 2012-02 |
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著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/16033 | |||||
識別子タイプ | HDL |