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  1. C100 医学部/医学系研究科
  2. C100b 紀要
  3. Nagoya journal of medical science
  4. 71(3-4)

Mutated RAS Induced PLD1 Gene Expression through Increased Sp1 Trascription Factor

https://doi.org/10.18999/nagjms.71.3-4.127
https://doi.org/10.18999/nagjms.71.3-4.127
a75d9cbd-6cb0-43d5-a5e8-27578191cf07
名前 / ファイル ライセンス アクション
p127-136_Gao.pdf p127-136_Gao.pdf (643.0 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2012-08-30
タイトル
タイトル Mutated RAS Induced PLD1 Gene Expression through Increased Sp1 Trascription Factor
著者 GAO, SIQIANG

× GAO, SIQIANG

WEKO 45848

GAO, SIQIANG

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MURAKAMI, MASASHI

× MURAKAMI, MASASHI

WEKO 45849

MURAKAMI, MASASHI

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ITO, HIROMI

× ITO, HIROMI

WEKO 45850

ITO, HIROMI

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FURUHATA, AYAKO

× FURUHATA, AYAKO

WEKO 45851

FURUHATA, AYAKO

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YOSHIDA, KAYO

× YOSHIDA, KAYO

WEKO 45852

YOSHIDA, KAYO

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TAGAWA, YOKO

× TAGAWA, YOKO

WEKO 45853

TAGAWA, YOKO

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HAGIWARA, KAZUMI

× HAGIWARA, KAZUMI

WEKO 45854

HAGIWARA, KAZUMI

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TAKAGI, AKIRA

× TAKAGI, AKIRA

WEKO 45855

TAKAGI, AKIRA

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KOJIMA, TETSUHITO

× KOJIMA, TETSUHITO

WEKO 45856

KOJIMA, TETSUHITO

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SUZUKI, MOTOSHI

× SUZUKI, MOTOSHI

WEKO 45857

SUZUKI, MOTOSHI

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BANNO, YOSHIKO

× BANNO, YOSHIKO

WEKO 45858

BANNO, YOSHIKO

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NOZAWA, YOSHINORI

× NOZAWA, YOSHINORI

WEKO 45859

NOZAWA, YOSHINORI

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MURATE, TAKASHI

× MURATE, TAKASHI

WEKO 45860

MURATE, TAKASHI

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キーワード
主題Scheme Other
主題 Mutated ras
キーワード
主題Scheme Other
主題 PLDI mRNA
キーワード
主題Scheme Other
主題 Sp1 transcription factor
キーワード
主題Scheme Other
主題 Promoter analysis
キーワード
主題Scheme Other
主題 ChIP assay
抄録
内容記述 The underlying mechanisms of oncogene-induced phospholipase D (PLD) activation have not been fully elucidated. The effect of the mutated-ras on PLD mRNA was examined using colon cancer cell lines as well as mock- and mutated ras-transfected NIH3T3 cells. Ras-mutation and activation were correlated, and cells with enhanced ras-activation showed increased PLD1 mRNA and protein. Analysis of the 5’ PLD1 promoter using a representative cell line, DLD-1 and also mutated ras-NIH3T3, showed one Sp1-site as the important ras-responsible motif. Sp1 inhibition with mithramycin A and Sp1 siRNA inhibited PLD1 protein expression and its promoter activity. Sp1 but not Sp3 protein level and increased Sp1-motif binding activity were correlated with ras ativation. Furthermore, overexpression of Sp1 in drosophila SL2 cells lacking Sp family proteins increased PLD1 promoter activity. EMSA and chromatin immunoprecipitation assay confirmed the importance of Sp1 protein binding to the Sp1-motif in ras-induced PLD1 mRNA expression.
内容記述タイプ Abstract
出版者
出版者 Nagoya University School of Medicine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/nagjms.71.3-4.127
ID登録タイプ JaLC
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 0027-7622
書誌情報 Nagoya Journal of Medical Science

巻 71, 号 3-4, p. 127-136, 発行日 2009-09
著者版フラグ
値 publisher
URI
識別子 http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/7134/7134.html
識別子タイプ URI
URI
識別子 http://hdl.handle.net/2237/16765
識別子タイプ HDL
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