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Mutated RAS Induced PLD1 Gene Expression through Increased Sp1 Trascription Factor
https://doi.org/10.18999/nagjms.71.3-4.127
https://doi.org/10.18999/nagjms.71.3-4.127a75d9cbd-6cb0-43d5-a5e8-27578191cf07
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
p127-136_Gao.pdf (643.0 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-08-30 | |||||
| タイトル | ||||||
| タイトル | Mutated RAS Induced PLD1 Gene Expression through Increased Sp1 Trascription Factor | |||||
| 言語 | en | |||||
| 著者 |
GAO, SIQIANG
× GAO, SIQIANG× MURAKAMI, MASASHI× ITO, HIROMI× FURUHATA, AYAKO× YOSHIDA, KAYO× TAGAWA, YOKO× HAGIWARA, KAZUMI× TAKAGI, AKIRA× KOJIMA, TETSUHITO× SUZUKI, MOTOSHI× BANNO, YOSHIKO× NOZAWA, YOSHINORI× MURATE, TAKASHI |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Mutated ras | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | PLDI mRNA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Sp1 transcription factor | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Promoter analysis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ChIP assay | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The underlying mechanisms of oncogene-induced phospholipase D (PLD) activation have not been fully elucidated. The effect of the mutated-ras on PLD mRNA was examined using colon cancer cell lines as well as mock- and mutated ras-transfected NIH3T3 cells. Ras-mutation and activation were correlated, and cells with enhanced ras-activation showed increased PLD1 mRNA and protein. Analysis of the 5’ PLD1 promoter using a representative cell line, DLD-1 and also mutated ras-NIH3T3, showed one Sp1-site as the important ras-responsible motif. Sp1 inhibition with mithramycin A and Sp1 siRNA inhibited PLD1 protein expression and its promoter activity. Sp1 but not Sp3 protein level and increased Sp1-motif binding activity were correlated with ras ativation. Furthermore, overexpression of Sp1 in drosophila SL2 cells lacking Sp family proteins increased PLD1 promoter activity. EMSA and chromatin immunoprecipitation assay confirmed the importance of Sp1 protein binding to the Sp1-motif in ras-induced PLD1 mRNA expression. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Nagoya University School of Medicine | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| ID登録 | ||||||
| ID登録 | 10.18999/nagjms.71.3-4.127 | |||||
| ID登録タイプ | JaLC | |||||
| 関連情報 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/7134/7134.html | |||||
| ISSN（print） | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0027-7622 | |||||
| ISSN（Online） | ||||||
| 収録物識別子タイプ | EISSN | |||||
| 収録物識別子 | 2186-3326 | |||||
| 書誌情報 |
en : Nagoya Journal of Medical Science 巻 71, 号 3-4, p. 127-136, 発行日 2009-09 |
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| 著者版フラグ | ||||||
| 値 | publisher | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/16765 | |||||
| 識別子タイプ | HDL | |||||
