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Studies on the Additional Peptide of Procollagen Extracted from Chick Embryo Cartilage
https://doi.org/10.18999/nagjms.40.1-2.37
https://doi.org/10.18999/nagjms.40.1-2.37de474b69-5322-46ff-b1f9-445c272a7b82
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
v40n12p37_46.pdf (813.1 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2013-01-23 | |||||
| タイトル | ||||||
| タイトル | Studies on the Additional Peptide of Procollagen Extracted from Chick Embryo Cartilage | |||||
| 言語 | en | |||||
| 著者 |
KUSAKABE, ATSUHIKO
× KUSAKABE, ATSUHIKO |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The additional peptide of procollagen is thought to play an important role in its synthesis, and attempts were made to elucidate the chemical properties of the additional peptide, particularly in regard to the possible existence of sugar substituents. Labeled procollagens were obtained with 4 M guanidine.HCl from chick embryo epiphyseal cartilages which had been incubated with [14C] proline, [14C] tryptophan or [14C] glucose. Each of the procollagen samples was digested with bacterial collagenase, and then chromatographed on 0.1% SDS.Sephadex G-150. The [14C] glucose-labeled sample yielded a fraction with the size of additional peptide (molecular weight, 13,200), plus collagenase-digestible small peptides. The peptide was distinct from the collagenase.sensitive region (α-region) in having [14C] tryptophan. On acid hydrolysis of the additional peptide samples, radioactive glucose, galactose, mannose and a small amount of glucosamine were recovered on paper chromatography and paper electrophoresis. Mannose and glucosamine are the components not found in the authentic α-chain of type II collagen. The results demonstrate that the additional peptide of cartilage procollagen is different from the α1(II) chain in amino acid composition and also in the type of sugars attached. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Nagoya University School of Medicine | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| ID登録 | ||||||
| ID登録 | 10.18999/nagjms.40.1-2.37 | |||||
| ID登録タイプ | JaLC | |||||
| 関連情報 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/4012/4012.html | |||||
| ISSN（print） | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0027-7622 | |||||
| ISSN（Online） | ||||||
| 収録物識別子タイプ | EISSN | |||||
| 収録物識別子 | 2186-3326 | |||||
| 書誌情報 |
en : Nagoya Journal of Medical Science 巻 40, 号 1-2, p. 37-46, 発行日 1978-01 |
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| 著者版フラグ | ||||||
| 値 | publisher | |||||
