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  1. C100 医学部/医学系研究科
  2. C100b 紀要
  3. Nagoya journal of medical science
  4. 46(1-4)

Two Components in L1210 Cells and Their Growth Characterization

https://doi.org/10.18999/nagjms.46.1-4.125
https://doi.org/10.18999/nagjms.46.1-4.125
3825e556-cdd7-4936-aac8-f1a14be2715a
名前 / ファイル ライセンス アクション
v46n14p125_142.pdf v46n14p125_142.pdf (1.3 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2013-02-08
タイトル
タイトル Two Components in L1210 Cells and Their Growth Characterization
著者 WAKAYAMA, KIKUKO

× WAKAYAMA, KIKUKO

WEKO 98697

WAKAYAMA, KIKUKO

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BESA, EMMANUEL C.

× BESA, EMMANUEL C.

WEKO 98698

BESA, EMMANUEL C.

Search repository
BASKIN, STEVEN I.

× BASKIN, STEVEN I.

WEKO 98699

BASKIN, STEVEN I.

Search repository
キーワード
主題Scheme Other
主題 L1210 cells
キーワード
主題Scheme Other
主題 Ficoll-Hypaque method
キーワード
主題Scheme Other
主題 Heterogeneity of leukemia
キーワード
主題Scheme Other
主題 Growth kinetics
キーワード
主題Scheme Other
主題 Taurine
抄録
内容記述 L1210 cells were separated into two populations by the standardized method of mononuclear cell purification using Ficoll-Hypaque. One population (L-cells) was obtained in the usual mononuclear layer above Ficoll-Hypaque and the other (Bo-cells) was found at the bottom of the tubes below Ficoll-Hypaque. The biological characteristics of both populations were evaluated by the following parameters at every 24 hr and up to 168 hr after intraperitoneal inoculation of 10^5 L1210 cells into DBA/2J mice: growth kinetics in vivo and in vitro, viability. morphological features, cell volume, 3H-thymidine (TdR) uptake, contents of protein. DNA and taurine, and modal chromosome number. Protein content was generally higher in Bo-cells than in L-cells throughout the observation period. The mean cell volume of Bo-cells increased and decreased more rapidly compared with L-cells, and its was significantly larger at earlier time points and smaller at later time points. DNA content and cell proliferation patterns in vitro and in vivo of Bo-cells showed similar changes. 3^H_TdR uptake was significantly higher in Bo-cells at any time point except for the last 168 hr, and taurine contents in both L- and Bo-cells showed almost mirrorimage values of their respective 3^H-TdR uptakes. Viability and cytological findings did not show any specific difference between L- and Bo-cells. These studies demonstrated the heterogeneity of L1210 cells, which until now have been believed to be very homogeneous tumor cells. The correlation between cell growth pattern and effectiveness of antineoplastic agents was also discussed.
内容記述タイプ Abstract
出版者
出版者 Nagoya University School of Medicine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/nagjms.46.1-4.125
ID登録タイプ JaLC
関連情報
関連タイプ isVersionOf
識別子タイプ URI
関連識別子 http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/4614/4614.html
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 0027-7622
ISSN(Online)
収録物識別子タイプ ISSN
収録物識別子 2186-3326
書誌情報 Nagoya Journal of Medical Science

巻 46, 号 1-4, p. 125-142, 発行日 1984-03
著者版フラグ
値 publisher
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