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  1. C100 医学部/医学系研究科
  2. C100b 紀要
  3. Nagoya journal of medical science
  4. 47(1-2)

Occurrence of a Terminal Deoxynucleotidyl Transferase-Like Activity in N-2-Fluorenylacetamide-treated Rat Liver

https://doi.org/10.18999/nagjms.47.1-2.43
https://doi.org/10.18999/nagjms.47.1-2.43
792ebdec-4370-4d3c-aafa-eff75c455cad
名前 / ファイル ライセンス アクション
v47n12p43_49.pdf v47n12p43_49.pdf (513.4 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2013-02-08
タイトル
タイトル Occurrence of a Terminal Deoxynucleotidyl Transferase-Like Activity in N-2-Fluorenylacetamide-treated Rat Liver
著者 YOSHIDA, SHONEN

× YOSHIDA, SHONEN

WEKO 47090

YOSHIDA, SHONEN

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NAKAMURA, HIROMU

× NAKAMURA, HIROMU

WEKO 47091

NAKAMURA, HIROMU

Search repository
KOJIMA, KIYOHIDE

× KOJIMA, KIYOHIDE

WEKO 47092

KOJIMA, KIYOHIDE

Search repository
キーワード
主題Scheme Other
主題 Terminal deoxynucleotidyl transferase
キーワード
主題Scheme Other
主題 Fluorenylacetamide
キーワード
主題Scheme Other
主題 Rat liver
キーワード
主題Scheme Other
主題 DNA polymerases
抄録
内容記述 A new enzyme activity that resembles terminal deoxynucleotidyl transferase (TdT-like activity) was found in rat liver. Upon treatment of rats with a hepatocarcinogen, N-2-fluorenylacetamide, the TdT-like activity increased in parallel with DNA polymerase α and γ. By phosphocellulose column chromatography, the TdT-like activity was separated from DNA polymerase α, β, and γ. With a poly(dA)-initiator, dGTP or dCTP was the most efficient substrate in the presence of Mg^2+ or Mn^2+, respectively. In the presence of Mg^2+, the enzyme utilized poly (dC) as an initiator most efficiently. The TdT-like activity differed from previously described terminal deoxynucleotidyl transferase (E.C.2.7.7.31.) in its Km value for dGTP and in its sensitivity to ATP or to an antibody against calf thymus terminal deoxynucleotidyl transferase.
内容記述タイプ Abstract
出版者
出版者 Nagoya University School of Medicine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/nagjms.47.1-2.43
ID登録タイプ JaLC
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 0027-7622
ISSN(Online)
収録物識別子タイプ ISSN
収録物識別子 2186-3326
書誌情報 Nagoya Journal of Medical Science

巻 47, 号 1-2, p. 43-49, 発行日 1985-01
著者版フラグ
値 publisher
URI
識別子 http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/4712/4712.html
識別子タイプ URI
URI
識別子 http://hdl.handle.net/2237/17476
識別子タイプ HDL
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