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  1. C100 医学部/医学系研究科
  2. C100b 紀要
  3. Nagoya journal of medical science
  4. 53(1-4)

Establishment and Characterization of Immortalized Non-Transplantable Mouse Mammary Cell Lines Cloned from a MMTV-induced Tumor Cell Line Cultured for A Long Duration

https://doi.org/10.18999/nagjms.53.1-4.9
https://doi.org/10.18999/nagjms.53.1-4.9
dd416a72-a325-42df-a3b8-bc8cfdf65518
名前 / ファイル ライセンス アクション
v53n14p9_21.pdf v53n14p9_21.pdf (2.1 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2013-02-14
タイトル
タイトル Establishment and Characterization of Immortalized Non-Transplantable Mouse Mammary Cell Lines Cloned from a MMTV-induced Tumor Cell Line Cultured for A Long Duration
著者 TAKENAKA, TOKUYA

× TAKENAKA, TOKUYA

WEKO 47261

TAKENAKA, TOKUYA

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SAGA, SHINSUKE

× SAGA, SHINSUKE

WEKO 47262

SAGA, SHINSUKE

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MIYAISHI, OSAMU

× MIYAISHI, OSAMU

WEKO 47263

MIYAISHI, OSAMU

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IMAI, MASAO

× IMAI, MASAO

WEKO 47264

IMAI, MASAO

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YOKOI, TOYOHARU

× YOKOI, TOYOHARU

WEKO 47265

YOKOI, TOYOHARU

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LU, JIN

× LU, JIN

WEKO 47266

LU, JIN

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WAJJWALKU, WORAWIDH

× WAJJWALKU, WORAWIDH

WEKO 47267

WAJJWALKU, WORAWIDH

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KUSAKABE, MORIAKI

× KUSAKABE, MORIAKI

WEKO 47268

KUSAKABE, MORIAKI

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TAGUCHI, OSAMU

× TAGUCHI, OSAMU

WEKO 47269

TAGUCHI, OSAMU

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MATSUYAMA, MUTSUSHI

× MATSUYAMA, MUTSUSHI

WEKO 47270

MATSUYAMA, MUTSUSHI

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HOSHINO, MUNEMITSU

× HOSHINO, MUNEMITSU

WEKO 47271

HOSHINO, MUNEMITSU

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キーワード
主題Scheme Other
主題 A MMTV-induced tumor cell line
キーワード
主題Scheme Other
主題 Contact inhibition
キーワード
主題Scheme Other
主題 Non-transplantable cell lines
キーワード
主題Scheme Other
主題 Oncogenes
抄録
内容記述 During the culturing of a mouse mammary tumor cell line, MuMT73, maintained in vitro for more than a decade, we found morphological heterogeneity in its cells; some showed contact inhibition in their growth, some formed domes and some grew criss cross and piled up. In trying to clone the cell line to isolate cells showing contact inhibition or dome formation, we were able to establish six clonal cell lines. These six cell lines were categorically divided into three groups according to their phenotypical behavior, Groups A, B and C. Group A (clones 1, 5 and 7) cells had a property of contact inhibition. They induced no tumor when injected into the subcutaneous tissue of the back, nor even when injected into the mammary fat pads or under the kidney capsule of syngenic or nude mice, and therefore were thought to be non-malignant in nature. They were positively stained by anti-keratin antiserum and had mouse mammary tumor viruses (MMTVs). Group B (clone 6) cells grew in a crisscross pattern and piled up, and they induced tumors when injected into the subcutaneous tissue of the back of mice. Group C (clones 3 and 4) cells formed domes in their growth and induced some tumors in the mammary fat pads and under the kidney capsule of KSN nude mice. In Southern blots with MMTV-env probe, numerous exogenous MMTV proviruses were detected in these cell lines. The insertion patterns of these proviruses in cells of non-malignant clonal lines (Group A) resembled those of malignant lines (Group B), except one band (about 26 Kb), but were considerably different from those of intermediate lines (Group C). On the other hand, no difference was detected in Southern blots with int-1 or int-2 probes among the non-malignant, intermediate and malignant clonal cell lines.
内容記述タイプ Abstract
内容記述
内容記述 名古屋大学博士学位論文 学位の種類 : 博士(医学)(課程) 学位授与年月日:平成2年11月22日 竹中徳哉氏の博士論文として提出された
内容記述タイプ Other
出版者
出版者 Nagoya University School of Medicine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/nagjms.53.1-4.9
ID登録タイプ JaLC
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 0027-7622
ISSN(Online)
収録物識別子タイプ ISSN
収録物識別子 2186-3326
書誌情報 Nagoya Journal of Medical Science

巻 53, 号 1-4, p. 9-21, 発行日 1991-03
著者版フラグ
値 publisher
URI
識別子 http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5314/5314.html
識別子タイプ URI
URI
識別子 http://hdl.handle.net/2237/17515
識別子タイプ HDL
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