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Isolation and Some Biochemical Properties of Porcine Pancreas Mitochondria
https://doi.org/10.18999/nagjms.54.1-4.41
https://doi.org/10.18999/nagjms.54.1-4.41c276ec53-8b16-4dc4-b828-ef9fcfb3400c
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2013-02-14 | |||||
タイトル | ||||||
タイトル | Isolation and Some Biochemical Properties of Porcine Pancreas Mitochondria | |||||
言語 | en | |||||
著者 |
SAKAI, YUZO
× SAKAI, YUZO× ADACHI, KAYO× HAYAKAWA, TETSUO× WAKABAYASHI, TAKASHI |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pancreas | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mitochondria | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Dibucaine | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Trypsin inhibitor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Phospholipase A2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bovine serum albumin | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Morphologically and functionally intact mitochondria were isolated from the porcine pancreas using a conventional, differential centrifugation method. The homogenate of the porcine pancreas made in a medium containing 0.25 M sucrose, 10 mM Tris-Cl, pH 7.4, 0.5% bovine serum albumin and 1 mM EDTA, in the presence or in the absence of dibucaine and trypsin inhibitor, was centrifuged for 10 min at 700xg. The supernatant was centrifuged for 10 min at 7000xg, and the pellet was washed three times. Trypsin activity of mitochondria isolated in the absence of dibucaine and trypsin inhibitor was as low as that of mitochondria isolated in the presence of dibucaine and trypsin inhibitor, and a major part of the activity remained inactivated. Phospholipase A2 activity of the former mitochondria was as low as that of the latter, and remained unchanged up to 8-10 hr at 4°C. The presence of bovine serum albumin and EDTA in the respiration medium was absolutely required to obtain good respiratory controls of those mitochondria. These data suggest that well-coupled mitochondria can be obtained from the pancreas by a conventional isolation procedure without activating the major part of trypsin. | |||||
言語 | en | |||||
出版者 | ||||||
出版者 | Nagoya University School of Medicine | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
ID登録 | ||||||
ID登録 | 10.18999/nagjms.54.1-4.41 | |||||
ID登録タイプ | JaLC | |||||
関連情報 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | URI | |||||
関連識別子 | http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5414/5414.html | |||||
ISSN(print) | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0027-7622 | |||||
ISSN(Online) | ||||||
収録物識別子タイプ | EISSN | |||||
収録物識別子 | 2186-3326 | |||||
書誌情報 |
en : Nagoya Journal of Medical Science 巻 54, 号 1-4, p. 41-57, 発行日 1992-03 |
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著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/17521 | |||||
識別子タイプ | HDL |