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  1. C100 医学部/医学系研究科
  2. C100b 紀要
  3. Nagoya journal of medical science
  4. 75(1-2)

Simple and Rapid Quantitation of 21 Bile Acids in Rat Serum and Liver by UPLC-MS-MS: Effect of High Fat Diet on Glycine Conjugates of Rat Bile Acids

https://doi.org/10.18999/nagjms.75.1-2.57
https://doi.org/10.18999/nagjms.75.1-2.57
0aae6abc-24f6-4aac-bff6-a5fdcd0d3b02
名前 / ファイル ライセンス アクション
07_Ishii.pdf 07_Ishii.pdf (2.1 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2013-03-01
タイトル
タイトル Simple and Rapid Quantitation of 21 Bile Acids in Rat Serum and Liver by UPLC-MS-MS: Effect of High Fat Diet on Glycine Conjugates of Rat Bile Acids
著者 SUZUKI, YUDAI

× SUZUKI, YUDAI

WEKO 47450

SUZUKI, YUDAI

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KANEKO, RINA

× KANEKO, RINA

WEKO 47451

KANEKO, RINA

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NOMURA, MINA

× NOMURA, MINA

WEKO 47452

NOMURA, MINA

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NAITO, HISAO

× NAITO, HISAO

WEKO 47453

NAITO, HISAO

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KITAMORI, KAZUYA

× KITAMORI, KAZUYA

WEKO 47454

KITAMORI, KAZUYA

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NAKAJIMA, TAMIE

× NAKAJIMA, TAMIE

WEKO 47455

NAKAJIMA, TAMIE

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OGAWA, TADASHI

× OGAWA, TADASHI

WEKO 47456

OGAWA, TADASHI

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HATTORI, HIDEKI

× HATTORI, HIDEKI

WEKO 47457

HATTORI, HIDEKI

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SENO, HIROSHI

× SENO, HIROSHI

WEKO 47458

SENO, HIROSHI

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ISHII, AKIRA

× ISHII, AKIRA

WEKO 47459

ISHII, AKIRA

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キーワード
主題Scheme Other
主題 UPLC
キーワード
主題Scheme Other
主題 tandem MS
キーワード
主題Scheme Other
主題 Bile acids
キーワード
主題Scheme Other
主題 Glycine conjugates
キーワード
主題Scheme Other
主題 Taurine conjugates
抄録
内容記述 In this report, we present a simple and rapid method for analysis of 21 kinds of bile acids and the conjugates in rat serum and liver samples by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) in the negative ionization mode, using cholic-2, 2, 4, 4-d4 acid as internal standard. After liquid-liguid extraction from serum and liver samples, specimens were analyzed by UPLC equipped with an Acquity TQD tandem quadrupole mass spectrometer. All of the 21 bile acids were sufficiently separated within 5 min. For most bile acids, calibration curves showed good linearities in the range of 0.25 to 5000 ng/mL for serum samples, 2.5 ng/g to 50 mg/g for liver samples. The limits of detection (LOD) were estimated to be less than 0.25 to 7.5 ng/mL in serum, less than 2.5 to 10 ng/g in liver samples. The present method was validated with respect to repeatability; the coefficient of variation (CV) values were less than 26.7% in the serum and 25.9% in the liver. In the animal study, we compared 21 bile acids in the serum and liver samples of the stroke-prone spontaneously hypertensive (SHRSP) rats fed with control (SP) diet or high-fat and high-cholesterol-containing (HFC) diet. By feeding with HFC diet, the glycine conjugates of some bile acids significantly increased and the taurine conjugate of ulsodeoxicolate (TUDC) decreased in serum and liver samples. Our results suggest that the change of bile acid profiles could be applied for the diagnosis of non-alcoholic fatty liver disease (NAFLD).
内容記述タイプ Abstract
出版者
出版者 Nagoya University School of Medicine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/nagjms.75.1-2.57
ID登録タイプ JaLC
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 0027-7622
書誌情報 Nagoya Journal of Medical Science

巻 75, 号 1-2, p. 57-71, 発行日 2013-02
著者版フラグ
値 publisher
URI
識別子 http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/7512/7512.html
識別子タイプ URI
URI
識別子 http://hdl.handle.net/2237/17602
識別子タイプ HDL
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