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Simple and Rapid Quantitation of 21 Bile Acids in Rat Serum and Liver by UPLC-MS-MS: Effect of High Fat Diet on Glycine Conjugates of Rat Bile Acids
https://doi.org/10.18999/nagjms.75.1-2.57
https://doi.org/10.18999/nagjms.75.1-2.570aae6abc-24f6-4aac-bff6-a5fdcd0d3b02
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
07_Ishii.pdf (2.1 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-03-01 | |||||
| タイトル | ||||||
| タイトル | Simple and Rapid Quantitation of 21 Bile Acids in Rat Serum and Liver by UPLC-MS-MS: Effect of High Fat Diet on Glycine Conjugates of Rat Bile Acids | |||||
| 言語 | en | |||||
| 著者 |
SUZUKI, YUDAI
× SUZUKI, YUDAI× KANEKO, RINA× NOMURA, MINA× NAITO, HISAO× KITAMORI, KAZUYA× NAKAJIMA, TAMIE× OGAWA, TADASHI× HATTORI, HIDEKI× SENO, HIROSHI× ISHII, AKIRA |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | UPLC | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | tandem MS | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Bile acids | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Glycine conjugates | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Taurine conjugates | |||||
| 抄録 | ||||||
| 内容記述 | In this report, we present a simple and rapid method for analysis of 21 kinds of bile acids and the conjugates in rat serum and liver samples by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) in the negative ionization mode, using cholic-2, 2, 4, 4-d4 acid as internal standard. After liquid-liguid extraction from serum and liver samples, specimens were analyzed by UPLC equipped with an Acquity TQD tandem quadrupole mass spectrometer. All of the 21 bile acids were sufficiently separated within 5 min. For most bile acids, calibration curves showed good linearities in the range of 0.25 to 5000 ng/mL for serum samples, 2.5 ng/g to 50 mg/g for liver samples. The limits of detection (LOD) were estimated to be less than 0.25 to 7.5 ng/mL in serum, less than 2.5 to 10 ng/g in liver samples. The present method was validated with respect to repeatability; the coefficient of variation (CV) values were less than 26.7% in the serum and 25.9% in the liver. In the animal study, we compared 21 bile acids in the serum and liver samples of the stroke-prone spontaneously hypertensive (SHRSP) rats fed with control (SP) diet or high-fat and high-cholesterol-containing (HFC) diet. By feeding with HFC diet, the glycine conjugates of some bile acids significantly increased and the taurine conjugate of ulsodeoxicolate (TUDC) decreased in serum and liver samples. Our results suggest that the change of bile acid profiles could be applied for the diagnosis of non-alcoholic fatty liver disease (NAFLD). | |||||
| 言語 | en | |||||
| 内容記述タイプ | Abstract | |||||
| 出版者 | ||||||
| 言語 | en | |||||
| 出版者 | Nagoya University School of Medicine | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| ID登録 | ||||||
| ID登録 | 10.18999/nagjms.75.1-2.57 | |||||
| ID登録タイプ | JaLC | |||||
| 関連情報 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/7512/7512.html | |||||
| ISSN（print） | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0027-7622 | |||||
| ISSN（Online） | ||||||
| 収録物識別子タイプ | EISSN | |||||
| 収録物識別子 | 2186-3326 | |||||
| 書誌情報 |
en : Nagoya Journal of Medical Science 巻 75, 号 1-2, p. 57-71, 発行日 2013-02 |
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| 著者版フラグ | ||||||
| 値 | publisher | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/17602 | |||||
| 識別子タイプ | HDL | |||||
