{"_buckets": {"deposit": "9e21d183-d68a-4c9c-9114-7812ff86bef0"}, "_deposit": {"created_by": 17, "id": "2001918", "owner": "17", "owners": [17], "owners_ext": {"displayname": "\u56f3\u66f8\u60c5\u5831\u4fc2", "username": "repository"}, "pid": {"revision_id": 0, "type": "depid", "value": "2001918"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:02001918", "sets": []}, "author_link": [], "control_number": "2001918", "item_1615768549627": {"attribute_name": "\u51fa\u7248\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_1629683748249": {"attribute_name": "\u65e5\u4ed8", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2022-01-13", "subitem_date_issued_type": "Available"}]}, "item_9_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-09-15", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "108446", "bibliographicVolumeNumber": "690", "bibliographic_titles": [{"bibliographic_title": "Archives of Biochemistry and Biophysics", "bibliographic_titleLang": "en"}]}]}, "item_9_description_4": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "A simple NMR method to analyze the data obtained by NMR titration experiment of amyloid formation inhibitors against uniformly 15N-labeled amyloid-\u03b2 1\u201342 peptide (A\u03b2(1\u201342)) was described. By using solution nuclear magnetic resonance (NMR) measurement, the simplest method for monitoring the effects of A\u03b2 fibrilization inhibitors is the NMR chemical shift perturbation (CSP) experiment using 15N-labeled A\u03b2(1\u201342). However, the flexible and dynamic nature of A\u03b2(1\u201342) monomer may hamper the interpretation of CSP data. Here we introduced principal component analysis (PCA) for visualizing and analyzing NMR data of A\u03b2(1\u201342) in the presence of amyloid inhibitors including high concentration osmolytes. We measured 1H\u201315N 2D spectra of A\u03b2(1\u201342) at various temperatures as well as of A\u03b2(1\u201342) with several inhibitors, and subjected all the data to PCA (PCA-HSQC). The PCA diagram succeeded in differentiating the various amyloid inhibitors, including epigallocatechin gallate (EGCg), rosmarinic acid (RA) and curcumin (CUR) from high concentration osmolytes. We hypothesized that the CSPs reflected the conformational equilibrium of intrinsically disordered A\u03b2(1\u201342) induced by weak inhibitor binding rather than the specific molecular interactions.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_9_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Elsevier", "subitem_publisher_language": "en"}]}, "item_9_relation_43": {"attribute_name": "\u95a2\u9023\u60c5\u5831", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.abb.2020.108446", "subitem_relation_type_select": "DOI"}}]}, "item_9_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a9 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/", "subitem_rights_language": "en"}]}, "item_9_source_id_7": {"attribute_name": "\u53ce\u9332\u7269\u8b58\u5225\u5b50", "attribute_value_mlt": [{"subitem_source_identifier": "0003-9861", "subitem_source_identifier_type": "PISSN"}]}, "item_access_right": {"attribute_name": "\u30a2\u30af\u30bb\u30b9\u6a29", "attribute_value_mlt": [{"subitem_access_right": "embargoed access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_f1cf"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Iwaya, Naoko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Goda, Natsuko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Matsuzaki, Mizuki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Narita, Akihiro", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Shigemitsu, Yoshiki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Tenno, Takeshi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Abe, Yoshito", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Hoshi, Minako", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Hiroaki, Hidekazu", "creatorNameLang": "en"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "date": [{"dateType": "Available", "dateValue": "2022-01-13"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ArchBB-Iwaya-accepted-fullversion.pdf", "filesize": [{"value": "1.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "application/pdf", "size": 1200000.0, "url": {"objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/2001918/files/ArchBB-Iwaya-accepted-fullversion.pdf"}, "version_id": "80896736-bf8d-49ab-a24c-a1145921ae7d"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Principal component analysis of data from NMR titration experiment of uniformly 15N labeled amyloid beta (1\u201342) peptide with osmolytes and phenolic compounds", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Principal component analysis of data from NMR titration experiment of uniformly 15N labeled amyloid beta (1\u201342) peptide with osmolytes and phenolic compounds", "subitem_title_language": "en"}]}, "item_type_id": "40001", "owner": "17", "path": ["1903/1904/1905"], "permalink_uri": "http://hdl.handle.net/2237/0002001918", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2022-01-13"}, "publish_date": "2022-01-13", "publish_status": "0", "recid": "2001918", "relation": {}, "relation_version_is_last": true, "title": ["Principal component analysis of data from NMR titration experiment of uniformly 15N labeled amyloid beta (1\u201342) peptide with osmolytes and phenolic compounds"], "weko_shared_id": -1}