| Item type |
itemtype_ver1(1) |
| 公開日 |
2022-01-26 |
| タイトル |
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|
タイトル |
High-resolution spatiotemporal transcriptome analyses during cellularization of rice endosperm unveil the earliest gene regulation critical for aleurone and starchy endosperm cell fate specification |
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言語 |
en |
| 著者 |
Takafuji, Yoshinori
Shimizu-Sato, Sae
Ta, Kim Nhung
Suzuki, Toshiya
Nosaka-Takahashi, Misuzu
Oiwa, Tetsuro
Kimura, Wakana
Katoh, Hirokazu
Fukai, Mao
Takeda, Shin
Sato, Yutaka
Hattori, Tsukaho
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| アクセス権 |
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|
アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利 |
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|
言語 |
en |
|
権利情報 |
This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s10265-021-01329-w |
| 内容記述 |
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|
内容記述タイプ |
Abstract |
|
内容記述 |
The major tissues of the cereal endosperm are the starchy endosperm (SE) in the inner and the aleurone layer (AL) at the outer periphery. The fates of the cells that comprise these tissues are determined according to positional information; however, our understanding of the underlying molecular mechanisms remains limited. Here, we conducted a high-resolution spatiotemporal analysis of the rice endosperm transcriptome during early cellularization. In rice, endosperm cellularization proceeds in a concentric pattern from a primary alveolus cell layer, such that developmental progression can be defined by the number of cell layers. Using laser-capture microdissection to obtain precise tissue sections, transcriptomic changes were followed through five histologically defined stages of cellularization from the syncytial to 3-cell layer (3 L) stage. In addition, transcriptomes were compared between the inner and the outermost peripheral cell layers. Large differences in the transcriptomes between stages and between the inner and the peripheral cells were found. SE attributes were expressed at the alveolus-cell-layer stage but were preferentially activated in the inner cell layers that resulted from periclinal division of the alveolus cell layer. Similarly, AL attributes started to be expressed only after the 2 L stage and were localized to the outermost peripheral cell layer. These results indicate that the first periclinal division of the alveolus cell layer is asymmetric at the transcriptome level, and that the cell-fate-specifying positional cues and their perception system are already operating before the first periclinal division. Several genes related to epidermal identity (i.e., type IV homeodomain-leucine zipper genes and wax biosynthetic genes) were also found to be expressed at the syncytial stage, but their expression was localized to the outermost peripheral cell layer from the 2 L stage onward. We believe that our findings significantly enhance our knowledge of the mechanisms underlying cell fate specification in rice endosperm. |
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言語 |
en |
| 出版者 |
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出版者 |
Springer |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連情報 |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.1007/s10265-021-01329-w |
| 収録物識別子 |
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|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0918-9440 |
| 書誌情報 |
en : Journal of Plant Research
巻 134,
号 5,
p. 1061-1081,
発行日 2021-09
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| ファイル公開日 |
|
|
日付 |
2022-09-01 |
|
日付タイプ |
Available |
High-resolution_spatiotemporal_transcriptome_analyses.pdf (3.6 MB)
Supplementary_Tables_takafuji_et_al.xlsx (299 KB)
