Item type |
itemtype_ver1(1) |
公開日 |
2022-03-09 |
タイトル |
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タイトル |
Regulated splicing of large exons is linked to phase‐separation of vertebrate transcription factors |
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言語 |
en |
著者 |
Kawachi, Toshihiko
Masuda, Akio
Yamashita, Yoshihiro
Takeda, Jun‐ichi
Ohkawara, Bisei
Ito, Mikako
Ohno, Kinji
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アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
内容記述 |
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内容記述タイプ |
Abstract |
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内容記述 |
Although large exons cannot be readily recognized by the spliceosome, many are evolutionarily conserved and constitutively spliced for inclusion in the processed transcript. Furthermore, whether large exons may be enriched in a certain subset of proteins, or mediate specific functions, has remained unclear. Here, we identify a set of nearly 3,000 SRSF3-dependent large constitutive exons (S3-LCEs) in human and mouse cells. These exons are enriched for cytidine-rich sequence motifs, which bind and recruit the splicing factors hnRNP K and SRSF3. We find that hnRNP K suppresses S3-LCE splicing, an effect that is mitigated by SRSF3 to thus achieve constitutive splicing of S3-LCEs. S3-LCEs are enriched in genes for components of transcription machineries, including mediator and BAF complexes, and frequently contain intrinsically disordered regions (IDRs). In a subset of analyzed S3-LCE-containing transcription factors, SRSF3 depletion leads to deletion of the IDRs due to S3-LCE exon skipping, thereby disrupting phase-separated assemblies of these factors. Cytidine enrichment in large exons introduces proline/serine codon bias in intrinsically disordered regions and appears to have been evolutionarily acquired in vertebrates. We propose that layered splicing regulation by hnRNP K and SRSF3 ensures proper phase-separation of these S3-LCE-containing transcription factors in vertebrates. |
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言語 |
en |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Published online; 4 October 2021 |
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言語 |
en |
出版者 |
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出版者 |
Wiley |
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言語 |
en |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
関連情報 |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.15252/embj.2020107485 |
収録物識別子 |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0261-4189 |
書誌情報 |
en : The EMBO Journal
巻 40,
号 22,
p. e107485,
発行日 2021-11-15
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ファイル公開日 |
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日付 |
2022-04-04 |
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日付タイプ |
Available |