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  1. C100 医学部/医学系研究科
  2. C100a 雑誌掲載論文
  3. 学術雑誌

Regulated splicing of large exons is linked to phase‐separation of vertebrate transcription factors

http://hdl.handle.net/2237/0002002176
http://hdl.handle.net/2237/0002002176
bd9664df-ab1a-46f9-92a5-575e6ee334a3
名前 / ファイル ライセンス アクション
EMBOJ-2020-107485R-MS_file.pdf EMBOJ-2020-107485R-MS_file.pdf (6.1 MB)
Item type itemtype_ver1(1)
公開日 2022-03-09
タイトル
タイトル Regulated splicing of large exons is linked to phase‐separation of vertebrate transcription factors
言語 en
著者 Kawachi, Toshihiko

× Kawachi, Toshihiko

en Kawachi, Toshihiko

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Masuda, Akio

× Masuda, Akio

en Masuda, Akio

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Yamashita, Yoshihiro

× Yamashita, Yoshihiro

en Yamashita, Yoshihiro

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Takeda, Jun‐ichi

× Takeda, Jun‐ichi

en Takeda, Jun‐ichi

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Ohkawara, Bisei

× Ohkawara, Bisei

en Ohkawara, Bisei

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Ito, Mikako

× Ito, Mikako

en Ito, Mikako

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Ohno, Kinji

× Ohno, Kinji

en Ohno, Kinji

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
内容記述
内容記述 Although large exons cannot be readily recognized by the spliceosome, many are evolutionarily conserved and constitutively spliced for inclusion in the processed transcript. Furthermore, whether large exons may be enriched in a certain subset of proteins, or mediate specific functions, has remained unclear. Here, we identify a set of nearly 3,000 SRSF3-dependent large constitutive exons (S3-LCEs) in human and mouse cells. These exons are enriched for cytidine-rich sequence motifs, which bind and recruit the splicing factors hnRNP K and SRSF3. We find that hnRNP K suppresses S3-LCE splicing, an effect that is mitigated by SRSF3 to thus achieve constitutive splicing of S3-LCEs. S3-LCEs are enriched in genes for components of transcription machineries, including mediator and BAF complexes, and frequently contain intrinsically disordered regions (IDRs). In a subset of analyzed S3-LCE-containing transcription factors, SRSF3 depletion leads to deletion of the IDRs due to S3-LCE exon skipping, thereby disrupting phase-separated assemblies of these factors. Cytidine enrichment in large exons introduces proline/serine codon bias in intrinsically disordered regions and appears to have been evolutionarily acquired in vertebrates. We propose that layered splicing regulation by hnRNP K and SRSF3 ensures proper phase-separation of these S3-LCE-containing transcription factors in vertebrates.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 Published online; 4 October 2021
言語 en
内容記述タイプ Other
出版者
言語 en
出版者 Wiley
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
関連情報
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.15252/embj.2020107485
収録物識別子
収録物識別子タイプ PISSN
収録物識別子 0261-4189
書誌情報 en : The EMBO Journal

巻 40, 号 22, p. e107485, 発行日 2021-11-15
ファイル公開日
日付 2022-04-04
日付タイプ Available
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