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  1. B200 工学部/工学研究科
  2. B200a 雑誌掲載論文
  3. 学術雑誌

Comparative gene expression analysis for pre-osteoblast MC3T3-E1 cells under non-adhesive culture toward osteocyte differentiation

http://hdl.handle.net/2237/0002002184
http://hdl.handle.net/2237/0002002184
ca230280-802b-4ae9-8457-e4d33750459f
名前 / ファイル ライセンス アクション
Manuscript_final.pdf Manuscript_final.pdf (322 KB)
Fig1.pdf Fig1.pdf (34 KB)
Fig2.pdf Fig2.pdf (37 KB)
Fig3.pdf Fig3.pdf (34 KB)
Fig4.pdf Fig4.pdf (29 KB)
Fig5.pdf Fig5.pdf (29 KB)
Item type itemtype_ver1(1)
公開日 2022-03-11
タイトル
タイトル Comparative gene expression analysis for pre-osteoblast MC3T3-E1 cells under non-adhesive culture toward osteocyte differentiation
言語 en
著者 Kim, Jeonghyun

× Kim, Jeonghyun

en Kim, Jeonghyun

Search repository
Kigami, Hiroyuki

× Kigami, Hiroyuki

en Kigami, Hiroyuki

Search repository
Adachi, Taiji

× Adachi, Taiji

en Adachi, Taiji

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
内容記述
内容記述 Osteocytes play an important role to modulate the bone remodeling and are also known as terminally differentiated cells originated from the osteoblast precursor cells, but its differentiation mechanism remains unclear. Since an efficient in vitro method to evoke the osteocyte differentiation from the osteoblast precursor cells has not been established, we conducted the comparative gene expression analysis for mouse pre-osteoblast MC3T3-E1 cells in order to elucidate the key factors to induce the osteocyte differentiation from the pre-osteoblast cells. In this study, we prepared four different culture environments by modulating their cell-substrate interaction and cell–cell interaction; (i) low and (ii) high cell density on the adhesive culture models, and (iii) low and (iv) high cell density on the non-adhesive floating culture models. By comparing these conditions in terms of cell-substrate and cell–cell interaction, we showed that the elimination of cell-substrate interaction under non-adhesive floating culture greatly up-regulated the gene expression of osteocyte markers in the pre-osteoblast cells. Moreover, the presence of moderate cell–cell interaction in the non-adhesive spheroid culture further enhanced the up-regulation of osteocyte markers for the pre-osteoblast cells. The results altogether suggest the most appropriate culture environment to induce the in vitro osteocyte differentiation of pre-osteoblast cells.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
関連情報
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.jbiosc.2021.09.004
収録物識別子
収録物識別子タイプ PISSN
収録物識別子 13891723
書誌情報 en : Journal of Bioscience and Bioengineering

巻 132, 号 6, p. 651-656, 発行日 2021-12
ファイル公開日
日付 2022-12-01
日付タイプ Available
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