| Item type |
itemtype_ver1(1) |
| 公開日 |
2023-06-20 |
| タイトル |
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|
タイトル |
Knockdown of endoplasmic reticulum chaperone BiP leads to the death of parvocellular AVP/CRH neurons in mice |
|
言語 |
en |
| 著者 |
Kawaguchi, Yohei
Hagiwara, Daisuke
Tsumura, Tetsuro
Miyata, Takashi
Kobayashi, Tomoko
Sugiyama, Mariko
Onoue, Takeshi
Yasuda, Yoshinori
Iwama, Shintaro
Suga, Hidetaka
Banno, Ryoichi
Grinevich, Valery
Arima, Hiroshi
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| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利 |
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|
言語 |
en |
|
権利情報 |
"This is the peer reviewed version of the following article: [Kawaguchi, Y, Hagiwara, D, Tsumura, T, et al. Knockdown of endoplasmic reticulum chaperone BiP leads to the death of parvocellular AVP/CRH neurons in mice. J Neuroendocrinol. 2023; 35(1):e13223. doi:10.1111/jne.13223], which has been published in final form at [https://doi.org/10.1111/jne.13223]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited." |
| 内容記述 |
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|
内容記述タイプ |
Abstract |
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内容記述 |
Arginine vasopressin (AVP) is expressed in both magnocellular (magnAVP) and parvocellular AVP (parvAVP) neurons of the paraventricular nucleus, and AVP colocalizes with corticotropin-releasing hormone (CRH) only in the parvocellular neurons. The immunoglobulin heavy chain binding protein (BiP) is a major endoplasmic reticulum (ER) chaperone which regulates the unfolded protein response under ER stress. We previously demonstrated that knockdown of BiP in magnAVP neurons exacerbated ER stress, which resulted in the autophagy-associated cell death of magnAVP neurons. Using the same approach, in the present study we examined the role of BiP in mouse parvAVP/CRH neurons. Our data demonstrate that BiP is expressed in mouse parvAVP/CRH neurons under nonstress conditions and is upregulated in proportion to the increase in CRH expression after adrenalectomy. For BiP knockdown in parvAVP/CRH neurons, we utilized a viral approach in combination with shRNA interference. Knockdown of BiP expression induced ER stress in parvAVP/CRH neurons, as reflected by the expression of C/EBP homologous protein. Furthermore, BiP knockdown led to the loss of parvAVP/CRH neurons after 4 weeks. In summary, our results demonstrate that BiP plays a pivotal role in parvAVP/CRH neurons, which function as neuroendocrine cells producing a large number of secretory proteins. |
|
言語 |
en |
| 出版者 |
|
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出版者 |
Wiley |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連情報 |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.1111/jne.13223 |
| 収録物識別子 |
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収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0953-8194 |
| 書誌情報 |
en : Journal of Neuroendocrinology
巻 35,
号 1,
p. e13223,
発行日 2023-01
|
| ファイル公開日 |
|
|
日付 |
2024-01-01 |
|
日付タイプ |
Available |
shBiP-parvAVP_manuscript_22-11-09_2.pdf (6.7 MB)
