Item type |
itemtype_ver1(1) |
公開日 |
2023-07-28 |
タイトル |
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タイトル |
Molecular Mechanism of Spectral Tuning by Chloride Binding in Monkey Green Sensitive Visual Pigment |
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言語 |
en |
著者 |
Fujimoto, Kazuhiro J.
Minowa, Fumika
Nishina, Michiya
Nakamura, Shunta
Ohashi, Sayaka
Katayama, Kota
Kandori, Hideki
Yanai, Takeshi
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アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
権利 |
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言語 |
en |
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権利情報 |
This document is the Accepted Manuscript version of a Published Work that appeared in final form in [The Journal of Physical Chemistry Letters], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [https://pubs.acs.org/articlesonrequest/AOR-9YVBFZT74EPYSYRXZ8KV].” |
内容記述 |
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内容記述タイプ |
Abstract |
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内容記述 |
The visual pigments of the cones perceive red, green, and blue colors. The monkey green (MG) pigment possesses a unique Cl– binding site; however, its relationship to the spectral tuning in green pigments remains elusive. Recently, FTIR spectroscopy revealed the characteristic structural modifications of the retinal binding site by Cl– binding. Herein, we report the computational structural modeling of MG pigments and quantum-chemical simulation to investigate its spectral redshift and physicochemical relevance when Cl– is present. Our protein structures reflect the previously suggested structural changes. AlphaFold2 failed to predict these structural changes. Excited-state calculations successfully reproduced the experimental red-shifted absorption energies, corroborating our protein structures. Electrostatic energy decomposition revealed that the redshift results from the His197 protonation state and conformations of Glu129, Ser202, and Ala308; however, Cl– itself contributes to the blueshift. Site-directed mutagenesis supported our analysis. These modeled structures may provide a valuable foundation for studying cone pigments. |
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言語 |
en |
出版者 |
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出版者 |
ACS Publications |
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言語 |
en |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
関連情報 |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1021/acs.jpclett.2c03619 |
収録物識別子 |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
1948-7185 |
書誌情報 |
en : The Journal of Physical Chemistry Letters
巻 14,
号 7,
p. 1784-1793,
発行日 2023-02-23
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ファイル公開日 |
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日付 |
2024-02-23 |
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日付タイプ |
Available |