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An ELISA for quantifying quail IgY and characterizing maternal IgY transfer to egg yolk in several quail strains
http://hdl.handle.net/2237/25103
http://hdl.handle.net/2237/251031dab2913-7dbb-44d9-9c96-f7f902bfbef1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
160422_VII-Manuscript.pdf ファイル公開：2017/07/01 (626.7 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2016-11-25 | |||||
| タイトル | ||||||
| タイトル | An ELISA for quantifying quail IgY and characterizing maternal IgY transfer to egg yolk in several quail strains | |||||
| 言語 | en | |||||
| 著者 |
Murai, Atsushi
× Murai, Atsushi× Kakiuchi, Misako× Hamano, Takahito× Kobayashi, Misato× Tsudzuki, Masaoki× Nakano, Mikiharu× Matsuda, Yoichi× Horio, Fumihiko |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Blood IgY | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Egg yolk | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ELISA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Maternal IgY transfer | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Strain difference | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Quail | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In avian species, maternal blood immunoglobulin Y (IgY) is transferred to the egg yolks of maturing oocytes, but the mechanism underlying this transfer is unknown. To gain insight into the mechanism of maternal IgY transfer in quail, we established an enzyme-linked immunosorbent assay (ELISA) for the quantitation of quail IgY. We characterized strain differences in blood and egg yolk IgY concentrations and exogenously injected IgY-Fc uptakes into egg yolks. A specific rabbit polyclonal antibody to quail IgY was raised for the ELISA. Blood and egg yolk IgY concentrations were determined in six quail strains (one inbred strain, L; four closed population strains, AWE, DB, PS, WE; one commercial strain, Commercial). The birds were also injected with digoxigenin-labeled quail IgY-Fc, and its uptakes into laid eggs were compared. The strain difference in blood and egg yolk IgY concentrations was at most 2.5-fold, between PS and AWE. The rank order of IgY concentrations was AWE, Commercial, DB, L ≥ WE ≥ PS. A significant positive correlation (|R| = 0.786) between individual blood IgY and egg yolk IgY and the concentrated egg yolk IgY (1.5–2-fold) against blood IgY was observed. Interestingly, there was a significant inverse correlation (|R| = 0.452) between injected IgY-Fc uptakes and the blood IgY concentration, implying competition of the injected IgY-Fc and blood IgY in the process of IgY uptake into egg yolks. In conclusion, we successfully determined blood and egg yolk IgY concentrations in various quail strains by a quail IgY-specific ELISA. The concentrated egg yolk IgY against the blood IgY and the inverse relationship of exogenous IgY-Fc uptake against the blood IgY supports the existence of a selective IgY transport mechanism in avian maturing oocytes. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/j.vetimm.2016.04.013 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0165-2427 | |||||
| 書誌情報 |
en : Veterinary Immunology and Immunopathology 巻 175, p. 16-23, 発行日 2016-07 |
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| 著者版フラグ | ||||||
| 値 | author | |||||
| URI | ||||||
| 識別子 | http://doi.org/10.1016/j.vetimm.2016.04.013 | |||||
| 識別子タイプ | DOI | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/25103 | |||||
| 識別子タイプ | HDL | |||||
