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Simvastatin inhibits CD44 fragmentation in chondrocytes
http://hdl.handle.net/2237/25104
http://hdl.handle.net/2237/251042c423e5e-e66a-4146-9691-88b30b541a39
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
manuscript.pdf ファイル公開:2017/08/25 (1.0 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2016-11-25 | |||||
| タイトル | ||||||
| タイトル | Simvastatin inhibits CD44 fragmentation in chondrocytes | |||||
| 言語 | en | |||||
| 著者 |
Terabe, Kenya
× Terabe, Kenya× Takahashi, Nobunori× Takemoto, Toki× Knudson, Warren× Ishiguro, Naoki× Kojima, Toshihisa |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Chondrocyte | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Pericellular matrix | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Statin | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | CD44 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Lipid raft | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ADAM10 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In human osteoarthritic chondrocytes, the hyaluronan receptor CD44 undergoes proteolytic cleavage at the cell surface. CD44 cleavage is thought to require transit of CD44 into cholesterol-rich lipid rafts. The purpose of this study was to investigate whether statins exert a protective effect on articular chondrocytes due to diminution of cholesterol. Three model systems of chondrocytes were examined including human HCS-2/8 chondrosarcoma cells, human osteoarthritic chondrocytes and normal bovine articular chondrocytes. Treatment with IL-1β + Oncostatin M resulted in a substantial increase in CD44 fragmentation in each of the three chondrocyte models. Pre-incubation with simvastatin prior to treatment with IL-1β + Oncostatin M decreased the level of CD44 fragmentation, decreased the proportion of CD44 that transits into the lipid raft fractions, decreased ADAM10 activity and diminished the interaction between CD44 and ADAM10. In HCS-2/8 cells and bovine articular chondrocytes, fragmentation of CD44 was blocked by the knockdown of ADAM10. Inhibition of CD44 fragmentation by simvastatin also resulted in improved retention of pericellular matrix. Addition of cholesterol and farnesyl-pyrophosphate reversed the protective effects of simvastatin. Thus, the addition of simvastatin exerts positive effects on chondrocytes including reduced CD44 fragmentation and enhanced the retention of pericellular matrix. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/j.abb.2016.05.019 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0003-9861 | |||||
| 書誌情報 |
en : Archives of Biochemistry and Biophysics 巻 604, p. 1-10, 発行日 2016-08-25 |
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| 著者版フラグ | ||||||
| 値 | author | |||||
| URI | ||||||
| 識別子 | http://doi.org/10.1016/j.abb.2016.05.019 | |||||
| 識別子タイプ | DOI | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/25104 | |||||
| 識別子タイプ | HDL | |||||
