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  1. B200 工学部/工学研究科
  2. B200a 雑誌掲載論文
  3. 学術雑誌

Shape-controlled high cell-density microcapsules by electrodeposition

http://hdl.handle.net/2237/25108
http://hdl.handle.net/2237/25108
3cea6884-7212-4a7c-8822-d25fd3bf08d6
名前 / ファイル ライセンス アクション
Paper_for_nagoya.pdf Paper_for_nagoya.pdf ファイル公開:2018/06/01 (2.5 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2016-11-25
タイトル
タイトル Shape-controlled high cell-density microcapsules by electrodeposition
言語 en
著者 Liu, Zeyang

× Liu, Zeyang

WEKO 67524

en Liu, Zeyang

Search repository
Takeuchi, Masaru

× Takeuchi, Masaru

WEKO 67525

en Takeuchi, Masaru

Search repository
Nakajima, Masahiro

× Nakajima, Masahiro

WEKO 67526

en Nakajima, Masahiro

Search repository
Hasegawa, Yasuhisa

× Hasegawa, Yasuhisa

WEKO 67527

en Hasegawa, Yasuhisa

Search repository
Huang, Qiang

× Huang, Qiang

WEKO 67528

en Huang, Qiang

Search repository
Fukuda, Toshio

× Fukuda, Toshio

WEKO 67529

en Fukuda, Toshio

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
抄録
内容記述 Cell encapsulation within alginate-poly-l-lysine (PLL) microcapsules has been developed to provide a miniaturized three-dimensional (3D) microenvironment with an aqueous core while promoting development of encapsulated cells into high cell-density structures. In this paper, a novel method for fabricating shape-controlled alginate-PLL microcapsules to construct 3D cell structures based on electrodeposition method is provided. Two-dimensional Ca-alginate cell-laden gel membranes were electrodeposited onto a micro-patterned electrode and further detached from the electrode. The PLL was coated onto the gel structures to form alginate-PLL complex as an outer shell and sodium citric solution was utilized to melt the internal alginate to achieve miniaturized 3D microcapsules (sphere, cuboid, and rod shape). By this proposed method, rat liver cells (RLC-18) formed multi-cellular aggregates with high cell-density after cultivation for 2 weeks.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.actbio.2016.03.045
ISSN
収録物識別子タイプ PISSN
収録物識別子 1742-7061
書誌情報 en : Acta Biomaterialia

巻 37, p. 93-100, 発行日 2016-06
著者版フラグ
値 author
URI
識別子 http://dx.doi.org/10.1016/j.actbio.2016.03.045
識別子タイプ DOI
URI
識別子 http://hdl.handle.net/2237/25108
識別子タイプ HDL
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