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  1. B200 工学部/工学研究科
  2. B200a 雑誌掲載論文
  3. 学術雑誌

Shape-controlled high cell-density microcapsules by electrodeposition

http://hdl.handle.net/2237/25108
3cea6884-7212-4a7c-8822-d25fd3bf08d6
名前 / ファイル ライセンス アクション
Paper_for_nagoya.pdf Paper_for_nagoya.pdf ファイル公開:2018/06/01 (2.5 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2016-11-25
タイトル
タイトル Shape-controlled high cell-density microcapsules by electrodeposition
著者 Liu, Zeyang

× Liu, Zeyang

WEKO 67524

Liu, Zeyang

Search repository
Takeuchi, Masaru

× Takeuchi, Masaru

WEKO 67525

Takeuchi, Masaru

Search repository
Nakajima, Masahiro

× Nakajima, Masahiro

WEKO 67526

Nakajima, Masahiro

Search repository
Hasegawa, Yasuhisa

× Hasegawa, Yasuhisa

WEKO 67527

Hasegawa, Yasuhisa

Search repository
Huang, Qiang

× Huang, Qiang

WEKO 67528

Huang, Qiang

Search repository
Fukuda, Toshio

× Fukuda, Toshio

WEKO 67529

Fukuda, Toshio

Search repository
権利
権利情報 © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
抄録
内容記述 Cell encapsulation within alginate-poly-l-lysine (PLL) microcapsules has been developed to provide a miniaturized three-dimensional (3D) microenvironment with an aqueous core while promoting development of encapsulated cells into high cell-density structures. In this paper, a novel method for fabricating shape-controlled alginate-PLL microcapsules to construct 3D cell structures based on electrodeposition method is provided. Two-dimensional Ca-alginate cell-laden gel membranes were electrodeposited onto a micro-patterned electrode and further detached from the electrode. The PLL was coated onto the gel structures to form alginate-PLL complex as an outer shell and sodium citric solution was utilized to melt the internal alginate to achieve miniaturized 3D microcapsules (sphere, cuboid, and rod shape). By this proposed method, rat liver cells (RLC-18) formed multi-cellular aggregates with high cell-density after cultivation for 2 weeks.
内容記述タイプ Abstract
出版者
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
ISSN
収録物識別子タイプ ISSN
収録物識別子 1742-7061
書誌情報 Acta Biomaterialia

巻 37, p. 93-100, 発行日 2016-06
著者版フラグ
値 author
URI
識別子 http://dx.doi.org/10.1016/j.actbio.2016.03.045
識別子タイプ DOI
URI
識別子 http://hdl.handle.net/2237/25108
識別子タイプ HDL
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