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Shape-controlled high cell-density microcapsules by electrodeposition
http://hdl.handle.net/2237/25108
3cea6884-7212-4a7c-8822-d25fd3bf08d6
名前 / ファイル | ライセンス | アクション | |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2016-11-25 | |||||
タイトル | ||||||
タイトル | Shape-controlled high cell-density microcapsules by electrodeposition | |||||
著者 |
Liu, Zeyang
× Liu, Zeyang× Takeuchi, Masaru× Nakajima, Masahiro× Hasegawa, Yasuhisa× Huang, Qiang× Fukuda, Toshio |
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権利 | ||||||
権利情報 | © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
抄録 | ||||||
内容記述 | Cell encapsulation within alginate-poly-l-lysine (PLL) microcapsules has been developed to provide a miniaturized three-dimensional (3D) microenvironment with an aqueous core while promoting development of encapsulated cells into high cell-density structures. In this paper, a novel method for fabricating shape-controlled alginate-PLL microcapsules to construct 3D cell structures based on electrodeposition method is provided. Two-dimensional Ca-alginate cell-laden gel membranes were electrodeposited onto a micro-patterned electrode and further detached from the electrode. The PLL was coated onto the gel structures to form alginate-PLL complex as an outer shell and sodium citric solution was utilized to melt the internal alginate to achieve miniaturized 3D microcapsules (sphere, cuboid, and rod shape). By this proposed method, rat liver cells (RLC-18) formed multi-cellular aggregates with high cell-density after cultivation for 2 weeks. | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
出版者 | Elsevier | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1742-7061 | |||||
書誌情報 |
Acta Biomaterialia 巻 37, p. 93-100, 発行日 2016-06 |
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著者版フラグ | ||||||
値 | author | |||||
URI | ||||||
識別子 | http://dx.doi.org/10.1016/j.actbio.2016.03.045 | |||||
識別子タイプ | DOI | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/25108 | |||||
識別子タイプ | HDL |