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A novel-type phosphatidylinositol phosphate-interactive, Ca-binding protein PCaP1 in Arabidopsisthaliana: stable association with plasma membrane and partial involvement in stomata closure
http://hdl.handle.net/2237/25111
http://hdl.handle.net/2237/2511133048497-a7b1-4c45-a5f9-c975d5dd007f
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
2016-JPR-Natata_et_al_25_411-417.pdf ファイル公開：2017/05/01 (969.4 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2016-11-28 | |||||
| タイトル | ||||||
| タイトル | A novel-type phosphatidylinositol phosphate-interactive, Ca-binding protein PCaP1 in Arabidopsisthaliana: stable association with plasma membrane and partial involvement in stomata closure | |||||
| 言語 | en | |||||
| 著者 |
Nagata, Chisako
× Nagata, Chisako× Miwa, Chika× Tanaka, Natsuki× Kato, Mariko× Suito, Momoe× Tsuchihira, Ayako× Sato, Yori× Segami, Shoji× Maeshima, Masayoshi |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | The final publication is available at Springer via http://doi.org/10.1007/s10265-016-0787-2 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Arabidopsis thaliana | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Calcium-binding protein | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Phosphatidylinositol phosphate | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Plasma membrane | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Signal transducing protein | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Stomata | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The Ca2+-binding protein-1 (PCaP1) of Arabidopsis thaliana is a new type protein that binds to phosphatidylinositol phosphates and Ca2+-calmodulin complex as well as free Ca2+. Although biochemical properties, such as binding to ligands and N-myristoylation, have been revealed, the intracellular localization, tissue and cell specificity, integrity of membrane association and physiological roles of PCaP1 are unknown. We investigated the tissue and intracellular distribution of PCaP1 by using transgenic lines expressing PCaP1 linked with a green fluorescence protein (GFP) at the carboxyl terminus of PCaP1. GFP fluorescence was obviously detected in most tissues including root, stem, leaf and flower. In these tissues, PCaP1–GFP signal was observed predominantly in the plasma membrane even under physiological stress conditions but not in other organelles. The fluorescence was detected in the cytosol when the 25-residue N-terminal sequence was deleted from PCaP1 indicating essential contribution of N-myristoylation to the plasma membrane anchoring. Fluorescence intensity of PCaP1–GFP in roots was slightly decreased in seedlings grown in medium supplemented with high concentrations of iron for 1 week and increased in those grown with copper. In stomatal guard cells, PCaP1–GFP was strictly, specifically localized to the plasma membrane at the epidermal-cell side but not at the pore side. A T-DNA insertion mutant line of PCaP1 did not show marked phenotype in a life cycle except for well growth under high CO2 conditions. However, stomata of the mutant line did not close entirely even in high osmolarity, which usually induces stomata closure. These results suggest that PCaP1 is involved in the stomatal movement, especially closure process, in leaves and response to excessive copper in root and leaf as a mineral nutrient as a physiological role. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Springer | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1007/s10265-016-0787-2 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0918-9440 | |||||
| 書誌情報 |
en : Journal of Plant Research 巻 129, 号 3, p. 539-550, 発行日 2016-05 |
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| 著者版フラグ | ||||||
| 値 | author | |||||
| URI | ||||||
| 識別子 | http://doi.org/10.1007/s10265-016-0787-2 | |||||
| 識別子タイプ | DOI | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/25111 | |||||
| 識別子タイプ | HDL | |||||
