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  1. B300 農学部/生命農学研究科
  2. B300a 雑誌掲載論文
  3. 学術雑誌

A novel-type phosphatidylinositol phosphate-interactive, Ca-binding protein PCaP1 in Arabidopsisthaliana: stable association with plasma membrane and partial involvement in stomata closure

http://hdl.handle.net/2237/25111
33048497-a7b1-4c45-a5f9-c975d5dd007f
名前 / ファイル ライセンス アクション
2016-JPR-Natata_et_al_25_411-417.pdf 2016-JPR-Natata_et_al_25_411-417.pdf ファイル公開:2017/05/01 (969.4 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2016-11-28
タイトル
タイトル A novel-type phosphatidylinositol phosphate-interactive, Ca-binding protein PCaP1 in Arabidopsisthaliana: stable association with plasma membrane and partial involvement in stomata closure
著者 Nagata, Chisako

× Nagata, Chisako

WEKO 67543

Nagata, Chisako

Search repository
Miwa, Chika

× Miwa, Chika

WEKO 67544

Miwa, Chika

Search repository
Tanaka, Natsuki

× Tanaka, Natsuki

WEKO 67545

Tanaka, Natsuki

Search repository
Kato, Mariko

× Kato, Mariko

WEKO 67546

Kato, Mariko

Search repository
Suito, Momoe

× Suito, Momoe

WEKO 67547

Suito, Momoe

Search repository
Tsuchihira, Ayako

× Tsuchihira, Ayako

WEKO 67548

Tsuchihira, Ayako

Search repository
Sato, Yori

× Sato, Yori

WEKO 67549

Sato, Yori

Search repository
Segami, Shoji

× Segami, Shoji

WEKO 67550

Segami, Shoji

Search repository
Maeshima, Masayoshi

× Maeshima, Masayoshi

WEKO 67551

Maeshima, Masayoshi

Search repository
権利
権利情報 The final publication is available at Springer via http://doi.org/10.1007/s10265-016-0787-2
キーワード
主題Scheme Other
主題 Arabidopsis thaliana
キーワード
主題Scheme Other
主題 Calcium-binding protein
キーワード
主題Scheme Other
主題 Phosphatidylinositol phosphate
キーワード
主題Scheme Other
主題 Plasma membrane
キーワード
主題Scheme Other
主題 Signal transducing protein
キーワード
主題Scheme Other
主題 Stomata
抄録
内容記述 The Ca2+-binding protein-1 (PCaP1) of Arabidopsis thaliana is a new type protein that binds to phosphatidylinositol phosphates and Ca2+-calmodulin complex as well as free Ca2+. Although biochemical properties, such as binding to ligands and N-myristoylation, have been revealed, the intracellular localization, tissue and cell specificity, integrity of membrane association and physiological roles of PCaP1 are unknown. We investigated the tissue and intracellular distribution of PCaP1 by using transgenic lines expressing PCaP1 linked with a green fluorescence protein (GFP) at the carboxyl terminus of PCaP1. GFP fluorescence was obviously detected in most tissues including root, stem, leaf and flower. In these tissues, PCaP1–GFP signal was observed predominantly in the plasma membrane even under physiological stress conditions but not in other organelles. The fluorescence was detected in the cytosol when the 25-residue N-terminal sequence was deleted from PCaP1 indicating essential contribution of N-myristoylation to the plasma membrane anchoring. Fluorescence intensity of PCaP1–GFP in roots was slightly decreased in seedlings grown in medium supplemented with high concentrations of iron for 1 week and increased in those grown with copper. In stomatal guard cells, PCaP1–GFP was strictly, specifically localized to the plasma membrane at the epidermal-cell side but not at the pore side. A T-DNA insertion mutant line of PCaP1 did not show marked phenotype in a life cycle except for well growth under high CO2 conditions. However, stomata of the mutant line did not close entirely even in high osmolarity, which usually induces stomata closure. These results suggest that PCaP1 is involved in the stomatal movement, especially closure process, in leaves and response to excessive copper in root and leaf as a mineral nutrient as a physiological role.
内容記述タイプ Abstract
出版者
出版者 Springer
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0918-9440
書誌情報 Journal of Plant Research

巻 129, 号 3, p. 539-550, 発行日 2016-05
著者版フラグ
値 author
URI
識別子 http://doi.org/10.1007/s10265-016-0787-2
識別子タイプ DOI
URI
識別子 http://hdl.handle.net/2237/25111
識別子タイプ HDL
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