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Contribution of PPi-Hydrolyzing Function of Vacuolar H+-Pyrophosphatase in Vegetative Growth of Arabidopsis: Evidenced by Expression of Uncoupling Mutated Enzymes
http://hdl.handle.net/2237/25112
http://hdl.handle.net/2237/2511290bbd11d-8f90-4382-b5bb-e6fd465e5a12
名前 / ファイル | ライセンス | アクション |
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2016-Front_Plant_Sci_asaoka.pdf (3.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2016-11-28 | |||||
タイトル | ||||||
タイトル | Contribution of PPi-Hydrolyzing Function of Vacuolar H+-Pyrophosphatase in Vegetative Growth of Arabidopsis: Evidenced by Expression of Uncoupling Mutated Enzymes | |||||
言語 | en | |||||
著者 |
Asaoka, Mariko
× Asaoka, Mariko× Segami, Shoji× Ferjani, Ali× Maeshima, Masayoshi |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | Copyright © 2016 Asaoka, Segami, Ferjani and Maeshima. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Arabidopsis thaliana | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | H+-pyrophosphatase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | pyrophosphate | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | proton pump | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | vacuole | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | plant growth | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The vacuolar-type H+-pyrophosphatase (H+-PPase) catalyzes a coupled reaction of pyrophosphate (PPi) hydrolysis and active proton translocation across the tonoplast. Overexpression of H+-PPase improves growth in various plant species, and loss-of-function mutants (fugu5s) of H+-PPase in Arabidopsis thaliana have post-germinative developmental defects. Here, to further clarify the physiological significance of this important enzyme, we newly generated three varieties of H+-PPase overexpressing lines with different levels of activity that we analyzed together with the loss-of-function mutant fugu5-3. The H+-PPase overexpressors exhibited enhanced activity of H+-PPase during vegetative growth, but no change in the activity of vacuolar H+-ATPase. Overexpressors with high enzymatic activity grew more vigorously with fresh weight increased by more than 24 and 44%, compared to the wild type and fugu5-3, respectively. Consistently, the overexpressors had larger rosette leaves and nearly 30% more cells in leaves than the wild type. When uncoupling mutated variants of H+-PPase, that could hydrolyze PPi but could not translocate protons, were introduced into the fugu5-3 mutant background, shoot growth defects recovered to the same levels as when a normal H+-PPase was introduced. Taken together, our findings clearly demonstrate that additional expression of H+-PPase improves plant growth by increasing cell number, predominantly as a consequence of the PPi-hydrolyzing activity of the enzyme. | |||||
言語 | en | |||||
出版者 | ||||||
出版者 | Frontiers | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.3389/fpls.2016.00415 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 1664-462X | |||||
書誌情報 |
en : Frontiers in Plant Science 巻 7, p. 415-415, 発行日 2016-03-31 |
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値 | publisher | |||||
URI | ||||||
識別子 | http://dx.doi.org/10.3389/fpls.2016.00415 | |||||
識別子タイプ | DOI | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/25112 | |||||
識別子タイプ | HDL |