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  1. B300 農学部/生命農学研究科
  2. B300a 雑誌掲載論文
  3. 学術雑誌

Contribution of PPi-Hydrolyzing Function of Vacuolar H+-Pyrophosphatase in Vegetative Growth of Arabidopsis: Evidenced by Expression of Uncoupling Mutated Enzymes

http://hdl.handle.net/2237/25112
http://hdl.handle.net/2237/25112
90bbd11d-8f90-4382-b5bb-e6fd465e5a12
名前 / ファイル ライセンス アクション
2016-Front_Plant_Sci_asaoka.pdf 2016-Front_Plant_Sci_asaoka.pdf (3.3 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2016-11-28
タイトル
タイトル Contribution of PPi-Hydrolyzing Function of Vacuolar H+-Pyrophosphatase in Vegetative Growth of Arabidopsis: Evidenced by Expression of Uncoupling Mutated Enzymes
言語 en
著者 Asaoka, Mariko

× Asaoka, Mariko

WEKO 67552

en Asaoka, Mariko

Search repository
Segami, Shoji

× Segami, Shoji

WEKO 67553

en Segami, Shoji

Search repository
Ferjani, Ali

× Ferjani, Ali

WEKO 67554

en Ferjani, Ali

Search repository
Maeshima, Masayoshi

× Maeshima, Masayoshi

WEKO 67555

en Maeshima, Masayoshi

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 Copyright © 2016 Asaoka, Segami, Ferjani and Maeshima. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
キーワード
主題Scheme Other
主題 Arabidopsis thaliana
キーワード
主題Scheme Other
主題 H+-pyrophosphatase
キーワード
主題Scheme Other
主題 pyrophosphate
キーワード
主題Scheme Other
主題 proton pump
キーワード
主題Scheme Other
主題 vacuole
キーワード
主題Scheme Other
主題 plant growth
抄録
内容記述 The vacuolar-type H+-pyrophosphatase (H+-PPase) catalyzes a coupled reaction of pyrophosphate (PPi) hydrolysis and active proton translocation across the tonoplast. Overexpression of H+-PPase improves growth in various plant species, and loss-of-function mutants (fugu5s) of H+-PPase in Arabidopsis thaliana have post-germinative developmental defects. Here, to further clarify the physiological significance of this important enzyme, we newly generated three varieties of H+-PPase overexpressing lines with different levels of activity that we analyzed together with the loss-of-function mutant fugu5-3. The H+-PPase overexpressors exhibited enhanced activity of H+-PPase during vegetative growth, but no change in the activity of vacuolar H+-ATPase. Overexpressors with high enzymatic activity grew more vigorously with fresh weight increased by more than 24 and 44%, compared to the wild type and fugu5-3, respectively. Consistently, the overexpressors had larger rosette leaves and nearly 30% more cells in leaves than the wild type. When uncoupling mutated variants of H+-PPase, that could hydrolyze PPi but could not translocate protons, were introduced into the fugu5-3 mutant background, shoot growth defects recovered to the same levels as when a normal H+-PPase was introduced. Taken together, our findings clearly demonstrate that additional expression of H+-PPase improves plant growth by increasing cell number, predominantly as a consequence of the PPi-hydrolyzing activity of the enzyme.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 Frontiers
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.3389/fpls.2016.00415
ISSN
収録物識別子タイプ PISSN
収録物識別子 1664-462X
書誌情報 en : Frontiers in Plant Science

巻 7, p. 415-415, 発行日 2016-03-31
著者版フラグ
値 publisher
URI
識別子 http://dx.doi.org/10.3389/fpls.2016.00415
識別子タイプ DOI
URI
識別子 http://hdl.handle.net/2237/25112
識別子タイプ HDL
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