ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "ec12992f-6496-4ae6-a8a1-efc3d2484276"}, "_deposit": {"id": "22933", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "22933"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00022933", "sets": ["667"]}, "author_link": ["67556", "67557", "67558", "67559", "67560", "67561", "67562", "67563"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2016-06-10", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "819", "bibliographicPageStart": "819", "bibliographicVolumeNumber": "7", "bibliographic_titles": [{"bibliographic_title": "Frontiers in Plant Science", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The plant vacuolar H+-pyrophosphatase (H+-PPase) functions as a proton pump coupled with the hydrolysis of pyrophosphate (PPi). Loss-of-function mutants (fugu5s and vhp1) of the H+-PPase of Arabidopsis thaliana show clear morphological phenotypes in the cotyledons, caused by inhibition of gluconeogenesis from seed storage lipids due to excessive accumulation of PPi. In this study, we investigated the phenotypes of the fugu5 and vhp1 mutants during vegetative growth under a specific nitrogen nutritional regime. When nitrate in the culture medium was the sole nitrogen source, growth of the mutant rosette leaves was severely compromised. Interestingly, trypan blue staining revealed notable cell death at the leaf blade–petiole junctions of young leaves, a region known to have meristematic features. Physical contact of the leaf tip with the culture medium also triggered leaf atrophy, suggesting that absorption of some elements through the hydathodes was probably involved in this phenotype. Prevention of such leaf–medium contact resulted in a marked decrease in phosphate content in the shoots, and suppressed leaf atrophy. Furthermore, fugu5 necrotic symptoms were rescued completely by heterologous expression of yeast cytosolic soluble pyrophosphatase IPP1 or uncoupling-type H+-PPases that retained only PPi-hydrolysis activity, indicating that the damage of actively proliferating cells was caused by the loss of the PPi-hydrolyzing function of H+-PPase. Importantly, cell death and growth defects of the fugu5 leaves were suppressed completely by the simple addition of ammonium (\u003e1 mM) to the culture medium. The PPi content in the shoots of fugu5 grown on ammonium-free medium was 70% higher than that of the wild type, and PPi levels were restored to normal upon growth on ammonium-supplemented medium. Together, these findings suggest that the PPi-hydrolyzing activity of H+-PPase is essential to maintain the PPi contents at optimal levels when grown on ammonium-free culture medium, and any direct contact of the leaves with the culture medium may raise PPi levels in the leaves through increased phosphate uptake.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "http://doi.org/10.3389/fpls.2016.00819"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/25113"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Frontiers", "subitem_publisher_language": "en"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.3389/fpls.2016.00819", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Copyright © 2016 Fukuda, Segami, Tomoyama, Asaoka, Nakanishi, Gunji, Ferjani and Maeshima. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.", "subitem_rights_language": "en"}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1664-462X", "subitem_source_identifier_type": "PISSN"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Fukuda, Mayu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "67556", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Segami, Shoji", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "67557", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tomoyama, Takaaki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "67558", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Asaoka, Mariko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "67559", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakanishi, Yoichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "67560", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Gunji, Shizuka", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "67561", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ferjani, Ali", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "67562", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Maeshima, Masayoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "67563", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-22"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "2016-Front_Plant_Sci_fukuda.pdf", "filesize": [{"value": "7.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 7800000.0, "url": {"label": "2016-Front_Plant_Sci_fukuda.pdf", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/22933/files/2016-Front_Plant_Sci_fukuda.pdf"}, "version_id": "06c3681b-d6b8-4667-b24b-d21e52cb09ea"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Arabidopsis thaliana", "subitem_subject_scheme": "Other"}, {"subitem_subject": "H+-pyrophosphatase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "pyrophosphate", "subitem_subject_scheme": "Other"}, {"subitem_subject": "proton pump", "subitem_subject_scheme": "Other"}, {"subitem_subject": "vacuole", "subitem_subject_scheme": "Other"}, {"subitem_subject": "plant growth", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Lack of H+-pyrophosphatase Prompts Developmental Damage in Arabidopsis Leaves on Ammonia-Free Culture Medium", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Lack of H+-pyrophosphatase Prompts Developmental Damage in Arabidopsis Leaves on Ammonia-Free Culture Medium", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["667"], "permalink_uri": "http://hdl.handle.net/2237/25113", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2016-11-28"}, "publish_date": "2016-11-28", "publish_status": "0", "recid": "22933", "relation": {}, "relation_version_is_last": true, "title": ["Lack of H+-pyrophosphatase Prompts Developmental Damage in Arabidopsis Leaves on Ammonia-Free Culture Medium"], "weko_shared_id": -1}
  1. B300 農学部/生命農学研究科
  2. B300a 雑誌掲載論文
  3. 学術雑誌

Lack of H+-pyrophosphatase Prompts Developmental Damage in Arabidopsis Leaves on Ammonia-Free Culture Medium

http://hdl.handle.net/2237/25113
http://hdl.handle.net/2237/25113
ff85192d-581a-43cd-882d-9c28bd68e906
名前 / ファイル ライセンス アクション
2016-Front_Plant_Sci_fukuda.pdf 2016-Front_Plant_Sci_fukuda.pdf (7.8 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2016-11-28
タイトル
タイトル Lack of H+-pyrophosphatase Prompts Developmental Damage in Arabidopsis Leaves on Ammonia-Free Culture Medium
言語 en
著者 Fukuda, Mayu

× Fukuda, Mayu

WEKO 67556

en Fukuda, Mayu

Search repository
Segami, Shoji

× Segami, Shoji

WEKO 67557

en Segami, Shoji

Search repository
Tomoyama, Takaaki

× Tomoyama, Takaaki

WEKO 67558

en Tomoyama, Takaaki

Search repository
Asaoka, Mariko

× Asaoka, Mariko

WEKO 67559

en Asaoka, Mariko

Search repository
Nakanishi, Yoichi

× Nakanishi, Yoichi

WEKO 67560

en Nakanishi, Yoichi

Search repository
Gunji, Shizuka

× Gunji, Shizuka

WEKO 67561

en Gunji, Shizuka

Search repository
Ferjani, Ali

× Ferjani, Ali

WEKO 67562

en Ferjani, Ali

Search repository
Maeshima, Masayoshi

× Maeshima, Masayoshi

WEKO 67563

en Maeshima, Masayoshi

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 Copyright © 2016 Fukuda, Segami, Tomoyama, Asaoka, Nakanishi, Gunji, Ferjani and Maeshima. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
キーワード
主題Scheme Other
主題 Arabidopsis thaliana
キーワード
主題Scheme Other
主題 H+-pyrophosphatase
キーワード
主題Scheme Other
主題 pyrophosphate
キーワード
主題Scheme Other
主題 proton pump
キーワード
主題Scheme Other
主題 vacuole
キーワード
主題Scheme Other
主題 plant growth
抄録
内容記述 The plant vacuolar H+-pyrophosphatase (H+-PPase) functions as a proton pump coupled with the hydrolysis of pyrophosphate (PPi). Loss-of-function mutants (fugu5s and vhp1) of the H+-PPase of Arabidopsis thaliana show clear morphological phenotypes in the cotyledons, caused by inhibition of gluconeogenesis from seed storage lipids due to excessive accumulation of PPi. In this study, we investigated the phenotypes of the fugu5 and vhp1 mutants during vegetative growth under a specific nitrogen nutritional regime. When nitrate in the culture medium was the sole nitrogen source, growth of the mutant rosette leaves was severely compromised. Interestingly, trypan blue staining revealed notable cell death at the leaf blade–petiole junctions of young leaves, a region known to have meristematic features. Physical contact of the leaf tip with the culture medium also triggered leaf atrophy, suggesting that absorption of some elements through the hydathodes was probably involved in this phenotype. Prevention of such leaf–medium contact resulted in a marked decrease in phosphate content in the shoots, and suppressed leaf atrophy. Furthermore, fugu5 necrotic symptoms were rescued completely by heterologous expression of yeast cytosolic soluble pyrophosphatase IPP1 or uncoupling-type H+-PPases that retained only PPi-hydrolysis activity, indicating that the damage of actively proliferating cells was caused by the loss of the PPi-hydrolyzing function of H+-PPase. Importantly, cell death and growth defects of the fugu5 leaves were suppressed completely by the simple addition of ammonium (>1 mM) to the culture medium. The PPi content in the shoots of fugu5 grown on ammonium-free medium was 70% higher than that of the wild type, and PPi levels were restored to normal upon growth on ammonium-supplemented medium. Together, these findings suggest that the PPi-hydrolyzing activity of H+-PPase is essential to maintain the PPi contents at optimal levels when grown on ammonium-free culture medium, and any direct contact of the leaves with the culture medium may raise PPi levels in the leaves through increased phosphate uptake.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 Frontiers
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.3389/fpls.2016.00819
ISSN
収録物識別子タイプ PISSN
収録物識別子 1664-462X
書誌情報 en : Frontiers in Plant Science

巻 7, p. 819-819, 発行日 2016-06-10
著者版フラグ
値 publisher
URI
識別子 http://doi.org/10.3389/fpls.2016.00819
識別子タイプ DOI
URI
識別子 http://hdl.handle.net/2237/25113
識別子タイプ HDL
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 14:46:20.075022
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3