{"_buckets": {"deposit": "3dceddbc-141a-46df-b1c9-1441951eead0"}, "_deposit": {"id": "23010", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "23010"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00023010"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2016-02-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "861", "bibliographicPageStart": "848", "bibliographicVolumeNumber": "57", "bibliographic_titles": [{"bibliographic_title": "Plant \u0026 Cell Physiology"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Progression of cell division is controlled by various mitotic kinases. In animal cells, phosphorylation of histone H3 at Thr3 by the kinase Haspin (haploid germ cell-specific nuclear protein kinase) promotes centromeric Aurora B localization to regulate chromosome segregation. However, less is known about the function of Haspin in regulatory networks in plant cells. Here, we show that inhibition of Haspin with 5-iodotubercidin (5-ITu) in Bright Yellow-2 (BY-2) cells delayed chromosome alignment. Haspin inhibition also prevented the centromeric localization of Aurora3 kinase (AUR3) and disrupted its function. This suggested that Haspin plays a role in the specific positioning of AUR3 on chromosomes in plant cells, a function conserved in animals. The results also indicated that Haspin and AUR3 are involved in the same pathway, which regulates chromosome alignment during prometaphase/metaphase. Remarkably, Haspin inhibition by 5-ITu also led to a severe cytokinesis defect, resulting in binuclear cells with a partially formed cell plate. The 5-ITu treatment did not affect microtubules, AUR1/2 or the NACK\u2013PQR pathway; however, it did alter the distribution of actin filaments on the cell plate. Together, these results suggested that Haspin has several functions in regulating cell division in plant cells: in the localization of AUR3 on centromeres and in regulating late cell plate expansion during cytokinesis.", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "http://doi.org/10.1093/pcp/pcw030"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/25194"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Oxford University Press"}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "This is a pre-copyedited, author-produced version of an article accepted for publication in [Plant \u0026 Cell Physiology] following peer review. The version of record [Plant \u0026 Cell Physiology. v.57, n.4, 2016, p.848-861] is available online at: http://doi.org/10.1093/pcp/pcw030."}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0032-0781", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kozgunova, Elena"}], "nameIdentifiers": [{"nameIdentifier": "67991", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Takamasa"}], "nameIdentifiers": [{"nameIdentifier": "67992", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ito, Masaki"}], "nameIdentifiers": [{"nameIdentifier": "67993", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Higashiyama, Tetsuya"}], "nameIdentifiers": [{"nameIdentifier": "67994", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kurihara, Daisuke"}], "nameIdentifiers": [{"nameIdentifier": "67995", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-02-12"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Kozgunova_et_al_2016_PCP.pdf", "filesize": [{"value": "28.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 28700000.0, "url": {"label": "Kozgunova_et_al_2016_PCP.pdf \u30d5\u30a1\u30a4\u30eb\u516c\u958b\uff1a2017/02/12", "url": "https://nagoya.repo.nii.ac.jp/record/23010/files/Kozgunova_et_al_2016_PCP.pdf"}, "version_id": "7c83316f-9001-49bb-b132-b2075a377edc"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Aurora3 kinase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "BY-2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Chromosome alignment", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cytokinesis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Haspin", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Haspin has Multiple Functions in the Plant Cell Division Regulatory Network", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Haspin has Multiple Functions in the Plant Cell Division Regulatory Network"}]}, "item_type_id": "10", "owner": "1", "path": ["336/695/696"], "permalink_uri": "http://hdl.handle.net/2237/25194", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2016-12-14"}, "publish_date": "2016-12-14", "publish_status": "0", "recid": "23010", "relation": {}, "relation_version_is_last": true, "title": ["Haspin has Multiple Functions in the Plant Cell Division Regulatory Network"], "weko_shared_id": null}