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Efficient In Planta Detection and Dissection of De Novo Mutation Events in the Arabidopsis thaliana Disease Resistance Gene UNI
http://hdl.handle.net/2237/25198
http://hdl.handle.net/2237/251987448b5d7-ad27-483b-ad68-07e09a45b8af
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2016-12-14 | |||||
タイトル | ||||||
タイトル | Efficient In Planta Detection and Dissection of De Novo Mutation Events in the Arabidopsis thaliana Disease Resistance Gene UNI | |||||
言語 | en | |||||
著者 |
Ogawa, Tomohiko
× Ogawa, Tomohiko× Mori, Akiko× Igari, Kadunari× Morita, Terao Miyo× Tasaka, Masao× Uchida, Naoyuki |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | This is a pre-copyedited, author-produced version of an article accepted for publication in [Plant & Cell Physiology] following peer review. The version of record [Plant and Cell Physiology. v.57, n.6, 2016, p.1123-1132] is available online at: http://doi.org/10.1093/pcp/pcw060. | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Arabidopsis thaliana | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA damage sensor pathway | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | R gene | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mutation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Salicylic acid pathway | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | UNI | |||||
抄録 | ||||||
内容記述 | Plants possess disease resistance (R) proteins encoded by R genes, and each R protein recognizes a specific pathogen factor(s) for immunity. Interestingly, a remarkably high degree of polymorphisms in R genes, which are traces of past mutation events during evolution, suggest the rapid diversification of R genes. However, little is known about molecular aspects that facilitate the rapid change of R genes because of the lack of tools that enable us to monitor de novo R gene mutations efficiently in an experimentally feasible time scale, especially in living plants. Here we introduce a model assay system that enables efficient in planta detection of de novo mutation events in the Arabidopsis thaliana R gene UNI in one generation. The uni-1D mutant harbors a gain-of-function allele of the UNI gene. uni-1D heterozygous individuals originally exhibit dwarfism with abnormally short stems. However, interestingly, morphologically normal stems sometimes emerge spontaneously from the uni-1D plants, and the morphologically reverted tissues carry additional de novo mutations in the UNI gene. Strikingly, under an extreme condition, almost half of the examined population shows the reversion phenomenon. By taking advantage of this phenomenon, we demonstrate that the reversion frequency is remarkably sensitive to a variety of fluctuations in DNA stability, underlying a mutable tendency of the UNI gene. We also reveal that activities of the salicylic acid pathway and DNA damage sensor pathway are involved in the reversion phenomenon. Thus, we provide an experimentally feasible model tool to explore factors and conditions that significantly affect the R gene mutation phenomenon. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Oxford University Press | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1093/pcp/pcw060 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0032-0781 | |||||
書誌情報 |
en : Plant and Cell Physiology 巻 57, 号 6, p. 1123-1132, 発行日 2016-03-25 |
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著者版フラグ | ||||||
値 | author | |||||
URI | ||||||
識別子 | http://doi.org/10.1093/pcp/pcw060 | |||||
識別子タイプ | DOI | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/25198 | |||||
識別子タイプ | HDL |