{"_buckets": {"deposit": "e379cf90-4d0f-437c-9797-cd317517eaee"}, "_deposit": {"id": "23238", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "23238"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00023238"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-02", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicPageEnd": "2464", "bibliographicPageStart": "2452", "bibliographicVolumeNumber": "35", "bibliographic_titles": [{"bibliographic_title": "The Journal of Neuroscience"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Engrafted mesenchymal stem cells from human deciduous dental pulp (SHEDs) support recovery from neural insults via paracrine mechanisms that are poorly understood. Here we show that the conditioned serum-free medium (CM) from SHEDs, administered intrathecally into rat injured spinal cord during the acute postinjury period, caused remarkable functional recovery. The ability of SHED-CM to induce recovery was associated with an immunoregulatory activity that induced anti-inflammatory M2-like macrophages. Secretome analysis of the SHED-CM revealed a previously unrecognized set of inducers for anti-inflammatory M2-like macrophages: monocyte chemoattractant protein-1 (MCP-1) and the secreted ectodomain of sialic acid-binding Ig-like lectin-9 (ED-Siglec-9). Depleting MCP-1 and ED-Siglec-9 from the SHED-CM prominently reduced its ability to induce M2-like macrophages and to promote functional recovery after spinal cord injury (SCI). The combination of MCP-1 and ED-Siglec-9 synergistically promoted the M2-like differentiation of bone marrow-derived macrophages in vitro, and this effect was abolished by a selective antagonist for CC chemokine receptor 2 (CCR2) or by the genetic knock-out of CCR2. Furthermore, MCP-1 and ED-Siglec-9 administration into the injured spinal cord induced M2-like macrophages and led to a marked recovery of hindlimb locomotor function after SCI. The inhibition of this M2 induction through the inactivation of CCR2 function abolished the therapeutic effects of both SHED-CM and MCP-1/ED-Siglec-9. Macrophages activated by MCP-1 and ED-Siglec-9 extended neurite and suppressed apoptosis of primary cerebellar granule neurons against the neurotoxic effects of chondroitin sulfate proteoglycans. Our data suggest that the unique combination of MCP-1 and ED-Siglec-9 repairs the SCI through anti-inflammatory M2-like macrophage induction.", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "https://doi.org/10.1523/JNEUROSCI.4088-14.2015"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/25433"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Society for Neuroscience"}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "Copyright(c)2015 the authors"}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0270-6474", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Matsubara, Kohki"}], "nameIdentifiers": [{"nameIdentifier": "68981", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsushita, Yoshihiro"}], "nameIdentifiers": [{"nameIdentifier": "68982", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sakai, Kiyoshi"}], "nameIdentifiers": [{"nameIdentifier": "68983", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kano, Fumiya"}], "nameIdentifiers": [{"nameIdentifier": "68984", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kondo, Megumi"}], "nameIdentifiers": [{"nameIdentifier": "68985", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Noda, Mariko"}], "nameIdentifiers": [{"nameIdentifier": "68986", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hashimoto, Noboru"}], "nameIdentifiers": [{"nameIdentifier": "68987", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Imagama, Shiro"}], "nameIdentifiers": [{"nameIdentifier": "68988", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ishiguro, Naoki"}], "nameIdentifiers": [{"nameIdentifier": "68989", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzumura, Akio"}], "nameIdentifiers": [{"nameIdentifier": "68990", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ueda, Minoru"}], "nameIdentifiers": [{"nameIdentifier": "68991", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Furukawa, Koichi"}], "nameIdentifiers": [{"nameIdentifier": "68992", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamamoto, Akihito"}], "nameIdentifiers": [{"nameIdentifier": "68993", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-22"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "2452_full.pdf", "filesize": [{"value": "6.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 6000000.0, "url": {"label": "2452_full.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/23238/files/2452_full.pdf"}, "version_id": "cf86b61c-6582-481a-b99d-9c958bc49b63"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "anti-inflammation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "dental pulp stem cells", "subitem_subject_scheme": "Other"}, {"subitem_subject": "macrophage polarity", "subitem_subject_scheme": "Other"}, {"subitem_subject": "MCP-1", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Siglec-9", "subitem_subject_scheme": "Other"}, {"subitem_subject": "spinal cord injury", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Secreted Ectodomain of Sialic Acid-Binding Ig-Like Lectin-9 and Monocyte Chemoattractant Protein-1 Promote Recovery after Rat Spinal Cord Injury by Altering Macrophage Polarity", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Secreted Ectodomain of Sialic Acid-Binding Ig-Like Lectin-9 and Monocyte Chemoattractant Protein-1 Promote Recovery after Rat Spinal Cord Injury by Altering Macrophage Polarity"}]}, "item_type_id": "10", "owner": "1", "path": ["499/500/501"], "permalink_uri": "http://hdl.handle.net/2237/25433", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2017-01-19"}, "publish_date": "2017-01-19", "publish_status": "0", "recid": "23238", "relation": {}, "relation_version_is_last": true, "title": ["Secreted Ectodomain of Sialic Acid-Binding Ig-Like Lectin-9 and Monocyte Chemoattractant Protein-1 Promote Recovery after Rat Spinal Cord Injury by Altering Macrophage Polarity"], "weko_shared_id": null}