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  1. F100 環境医学研究所
  2. F100a 雑誌掲載論文
  3. 学術雑誌

Optogenetic control of cell differentiation in channelrhodopsin-2-expressing OS3, a bipotential glial progenitor cell line

http://hdl.handle.net/2237/26609
http://hdl.handle.net/2237/26609
a8461a29-5fb2-4bc3-8130-1a4be5479eb8
名前 / ファイル ライセンス アクション
NCI.pdf NCI.pdf ファイル公開:2018/03/01 (20.5 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2017-06-08
タイトル
タイトル Optogenetic control of cell differentiation in channelrhodopsin-2-expressing OS3, a bipotential glial progenitor cell line
言語 en
著者 Ono, Kenji

× Ono, Kenji

WEKO 72276

en Ono, Kenji

Search repository
Suzuki, Hiromi

× Suzuki, Hiromi

WEKO 72277

en Suzuki, Hiromi

Search repository
Yamamoto, Ryusei

× Yamamoto, Ryusei

WEKO 72278

en Yamamoto, Ryusei

Search repository
Sahashi, Hideki

× Sahashi, Hideki

WEKO 72279

en Sahashi, Hideki

Search repository
Takido, Yuhei

× Takido, Yuhei

WEKO 72280

en Takido, Yuhei

Search repository
Sawada, Makoto

× Sawada, Makoto

WEKO 72281

en Sawada, Makoto

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
キーワード
主題Scheme Other
主題 Oligodendrocyte
キーワード
主題Scheme Other
主題 PI3K/Akt signaling
キーワード
主題Scheme Other
主題 Intracellular ion environment
キーワード
主題Scheme Other
主題 Channelrhodopsin-2
キーワード
主題Scheme Other
主題 Photo-activation
抄録
内容記述 Alterations in the intracellular ion environment have been identified as one of the signals playing a critical role in the control of cellular proliferation and differentiation; however, the mechanisms responsible for signal transduction remain unclear. Recent studies have reported that channelrhodopsin-2 (ChR2) is a rapidly gated blue light (BL)-sensitive cation channel suitable for the non-invasive control of ion influx. We herein examined the expression of differentiation-associated markers by photo-activation and its signal transduction in ChR2-expressing OS3 (OS3ChR2) cells, which are clonal bipotential glial progenitor cells. Increases were observed in intracellular Na+ and Ca2+ concentrations in OS3ChR2 cells with BL exposure. Alterations in the intracellular ion environment, particularly in Ca2+, led to increases in the expression of oligodendrocyte markers including galactocerebrosides (GalC) and decreases in that of astrocyte markers such as glial fibrillary acidic protein (GFAP). These alterations also triggered activation of the ERK1/2 signaling pathway, which is involved in cell survival, and PI3K/Akt/mTOR signaling pathway, which is involved in oligodendrocyte differentiation, characterized by GalC expression. Moreover, when photo-activated OS3ChR2 cells were injected into mice with lysophosphatidyl choline (LPC)-induced demyelination, deficits in motor function were reduced. Our results demonstrated that signal transduction by ChR2-expressing glial progenitor cells may be controlled through alterations induced in the intracellular ion environment by photo-activation and results in oligodendrocyte differentiation from glial progenitor cells. Our results also suggest that ChR2-expressing glial progenitor cells have potential as a useful tool for therapeutic approaches to brain and spinal cord disorders associated with oligodendrocyte dysfunctions.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.neuint.2016.12.022
ISSN
収録物識別子タイプ PISSN
収録物識別子 0197-0186
書誌情報 en : Neurochemistry International

巻 104, p. 49-63, 発行日 2017-03
著者版フラグ
値 author
URI
識別子 https://doi.org/10.1016/j.neuint.2016.12.022
識別子タイプ DOI
URI
識別子 http://hdl.handle.net/2237/26609
識別子タイプ HDL
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