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Chitin synthase 1 gene is crucial to antifungal host defense of the model beetle, Tribolium castaneum
http://hdl.handle.net/2237/26640
http://hdl.handle.net/2237/26640d7c7b3c2-3727-4983-9dfa-bc1c63a72d18
名前 / ファイル | ライセンス | アクション |
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JIP-16-240R1.pdf ファイル公開:2018/02/01 (840.3 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-06-09 | |||||
タイトル | ||||||
タイトル | Chitin synthase 1 gene is crucial to antifungal host defense of the model beetle, Tribolium castaneum | |||||
言語 | en | |||||
著者 |
Hayakawa, Yuuki
× Hayakawa, Yuuki× Kato, Daiki× Kamiya, Katsumi× Minakuchi, Chieka× Miura, Ken |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
抄録 | ||||||
内容記述 | The importance of the insect cuticle as a primary protective barrier against entomopathogens has long been noted. In the present study, we addressed this issue by utilizing an experimental infection system composed of the model beetle T. castaneum and two entomopathogenic fungal species, Beauveria bassiana and Metarhizium anisopliae. The pupae were relatively susceptible to these fungi by the natural route of infection, with some refractoriness developed with age, while the adults exhibited much higher refractoriness. Whereas M. anisopliae exhibited seemingly higher infectivity to the pupae compared to B. bassiana when the natural conidium infection was employed, direct inoculation of cultured hyphal body cells into the hemocoel was found highly and equally virulent in the pupae for the both fungal species. These results collectively suggest an important role of the cuticular integument in antifungal host defense, and we subsequently conducted the knockdown of chitin synthase 1 gene (CHS1). We targeted the prepupal and mid-pupal peaks of its expression respectively by using injection of the dsRNA at very low dosages to avoid lethality. The resulting pupae looked normal, but the adults showed a mild phenotype with dimpled/wrinkled elytra. The CHS1 gene knockdown compromised significantly host defense against the fungal infection via the natural route, except the configuration of knockdown pupae and M. anisopliae, suggesting an indispensable role of CHS1. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
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言語 | en | |||||
出版者 | Elsevier | |||||
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言語 | eng | |||||
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資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
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出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.jip.2016.11.009 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0022-2011 | |||||
書誌情報 |
en : Journal of Invertebrate Pathology 巻 143, p. 26-34, 発行日 2017-02 |
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値 | author | |||||
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識別子 | https://doi.org/10.1016/j.jip.2016.11.009 | |||||
識別子タイプ | DOI | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/26640 | |||||
識別子タイプ | HDL |