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  1. B300 農学部/生命農学研究科
  2. B300a 雑誌掲載論文
  3. 学術雑誌

Chitin synthase 1 gene is crucial to antifungal host defense of the model beetle, Tribolium castaneum

http://hdl.handle.net/2237/26640
http://hdl.handle.net/2237/26640
d7c7b3c2-3727-4983-9dfa-bc1c63a72d18
名前 / ファイル ライセンス アクション
JIP-16-240R1.pdf JIP-16-240R1.pdf ファイル公開:2018/02/01 (840.3 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2017-06-09
タイトル
タイトル Chitin synthase 1 gene is crucial to antifungal host defense of the model beetle, Tribolium castaneum
言語 en
著者 Hayakawa, Yuuki

× Hayakawa, Yuuki

WEKO 72388

en Hayakawa, Yuuki

Search repository
Kato, Daiki

× Kato, Daiki

WEKO 72389

en Kato, Daiki

Search repository
Kamiya, Katsumi

× Kamiya, Katsumi

WEKO 72390

en Kamiya, Katsumi

Search repository
Minakuchi, Chieka

× Minakuchi, Chieka

WEKO 72391

en Minakuchi, Chieka

Search repository
Miura, Ken

× Miura, Ken

WEKO 72392

en Miura, Ken

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
抄録
内容記述 The importance of the insect cuticle as a primary protective barrier against entomopathogens has long been noted. In the present study, we addressed this issue by utilizing an experimental infection system composed of the model beetle T. castaneum and two entomopathogenic fungal species, Beauveria bassiana and Metarhizium anisopliae. The pupae were relatively susceptible to these fungi by the natural route of infection, with some refractoriness developed with age, while the adults exhibited much higher refractoriness. Whereas M. anisopliae exhibited seemingly higher infectivity to the pupae compared to B. bassiana when the natural conidium infection was employed, direct inoculation of cultured hyphal body cells into the hemocoel was found highly and equally virulent in the pupae for the both fungal species. These results collectively suggest an important role of the cuticular integument in antifungal host defense, and we subsequently conducted the knockdown of chitin synthase 1 gene (CHS1). We targeted the prepupal and mid-pupal peaks of its expression respectively by using injection of the dsRNA at very low dosages to avoid lethality. The resulting pupae looked normal, but the adults showed a mild phenotype with dimpled/wrinkled elytra. The CHS1 gene knockdown compromised significantly host defense against the fungal infection via the natural route, except the configuration of knockdown pupae and M. anisopliae, suggesting an indispensable role of CHS1.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.jip.2016.11.009
ISSN
収録物識別子タイプ PISSN
収録物識別子 0022-2011
書誌情報 en : Journal of Invertebrate Pathology

巻 143, p. 26-34, 発行日 2017-02
著者版フラグ
値 author
URI
識別子 https://doi.org/10.1016/j.jip.2016.11.009
識別子タイプ DOI
URI
識別子 http://hdl.handle.net/2237/26640
識別子タイプ HDL
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