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Intraprostatic Reflux of Urine Induces Inflammation in a Rat
http://hdl.handle.net/2237/26643
http://hdl.handle.net/2237/2664337aaef02-1f91-4c5b-9009-c029a6dd5210
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-06-09 | |||||
| タイトル | ||||||
| タイトル | Intraprostatic Reflux of Urine Induces Inflammation in a Rat | |||||
| 言語 | en | |||||
| 著者 |
Funahashi, Yasuhito
× Funahashi, Yasuhito× Majima, Tsuyoshi× Matsukawa, Yoshihisa× Yamamoto, Tokunori× Yoshida, Masaki× Gotoh, Momokazu |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | This is the peer reviewed version of the following article: [Funahashi, Y., Majima, T., Matsukawa, Y., Yamamoto, T., Yoshida, M. and Gotoh, M. (2017), Intraprostatic Reflux of Urine Induces Inflammation in a Rat. Prostate, 77: 164–172. doi:10.1002/pros.23257], which has been published in final form at [http://doi.org/10.1002/pros.23257]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | BACKGROUND: We examined whether urine reflux into the prostate can induce prostatic inflammation in a rat and evaluated the effect of α1-adrenoreceptor antagonist. METHODS: Experiment 1: Male Sprague-Dawley rats were injected with 500 µl of Evans Blue through the urethral orifice. Intravesical pressure was measured, and the prostate was excised to evaluate urine reflux. Experiment 2: Rats were injected with 500 µl urine or saline (control) from the urethral orifice. Silodosin (200 µg/kg/day) was administered to the silodosin group. We evaluated histopathology, the expression of proinflammatory cytokines and oxidative stress markers of the prostate on day 7, after assessing the prostatic microcirculation and cystometrogram. RESULTS: Experiment 1: The histopathology showed that Evans Blue instilled through the urethral orifice entered the prostatic ducts. Intravesical pressure during Evans Blue instillation was 47.7 ± 1.6 cmH2O (mean ± standard error). Experiment 2: On day 7 after urine instillation through the urethral orifice, histopathology showed infiltrated inflammatory cells in the peri-glandular stroma. Inflammation-associated proteins (IL-1α, IL-1β, IL-6, and TNFα) were upregulated in the urine-instilled rats but not in the silodosin group. Erythrocyte speed on the prostatic surface, immunostaining for hypoxyprobe, and quantification of oxidative stress markers (MDA and HIF-1α) demonstrated prostatic hypoxia in the urine-instilled rats, which was ameliorated in the silodosin group. Cystometrogram revealed a shorter intercontraction interval in the urine-instilled rats, which was prolonged in the silodosin group. CONCLUSIONS: Urine reflux into the prostatic duct induces abacterial prostatitis. Silodosin relieved prostatic inflammation and bladder overactivity by increasing microcirculation in the prostate. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Wiley | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1002/pros.23257 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0270-4137 | |||||
| 書誌情報 |
en : Prostate 巻 77, 号 2, p. 164-172, 発行日 2017-02-01 |
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| 著者版フラグ | ||||||
| 値 | author | |||||
| URI | ||||||
| 識別子 | http://doi.org/10.1002/pros.23257 | |||||
| 識別子タイプ | DOI | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/26643 | |||||
| 識別子タイプ | HDL | |||||
