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  1. B300 農学部/生命農学研究科
  2. B300a 雑誌掲載論文
  3. 学術雑誌

The major constituents of the venom gland of a braconid endoparasitoid, Meteorus pulchricornis (Hymenoptera: Braconidae)

http://hdl.handle.net/2237/26859
http://hdl.handle.net/2237/26859
080a45c5-e7f7-4e78-9deb-4bfaaeaa86be
名前 / ファイル ライセンス アクション
AEAZ-D-16-00191_R1.pdf AEAZ-D-16-00191_R1.pdf ファイル公開:2018/05/01 (2.4 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2017-08-02
タイトル
タイトル The major constituents of the venom gland of a braconid endoparasitoid, Meteorus pulchricornis (Hymenoptera: Braconidae)
言語 en
著者 Yokoi, Kakeru

× Yokoi, Kakeru

WEKO 72973

en Yokoi, Kakeru

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Sano, Takeshi

× Sano, Takeshi

WEKO 72974

en Sano, Takeshi

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Suzuki, Masahiro

× Suzuki, Masahiro

WEKO 72975

en Suzuki, Masahiro

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Tanaka, Toshiharu

× Tanaka, Toshiharu

WEKO 72976

en Tanaka, Toshiharu

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Minakuchi, Chieka

× Minakuchi, Chieka

WEKO 72977

en Minakuchi, Chieka

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Miura, Ken

× Miura, Ken

WEKO 72978

en Miura, Ken

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 The final publication is available at Springer via http://doi.org/10.1007/s13355-016-0476-6
キーワード
主題Scheme Other
主題 Endoparasitoid wasp
キーワード
主題Scheme Other
主題 Virus-like particle
キーワード
主題Scheme Other
主題 Meteorus pulchricornis
キーワード
主題Scheme Other
主題 Mythimna separata
キーワード
主題Scheme Other
主題 RNA interference
抄録
内容記述タイプ Abstract
内容記述 The endoparasitoid wasp, Meteorus pulchricornis (Wesmael), introduces virus-like particles [M. pulchricornis VLP (MpVLP)] and venom into its lepidopteran host larvae upon oviposition. These wasp-associated factors are considered to help impair some host functions including immune defense. To obtain the major protein repertoire of the MpVLP and venom that might support successful parasitism, we constructed a conventional cDNA library of the venom gland filament that produces both of them, and sequenced cDNA clones arbitrarily in the 5′ regions. The 5′ expressed sequence tags obtained from 473 independent cDNA clones were grouped into 228 clusters. One hundred and five of these clusters were annotated with one or more gene ontology (GO) terms by Blast2GO analyses. While the major repertoire of the M. pulchricornis venom gland shared some constituents with those of the venom glands of other parasitoid wasp species, it was distinct from those encoded by polydnaviruses. Twenty clusters selected for further analyses were fully sequenced and characterized again. Among them, 17 factors were subjected to quantitative reverse transcription polymerase chain reaction analyses, and 12 factors were suggested to be highly adult specific. Among these 17 factors, ten were tested as RNA interference targets, and effective gene silencing was observed for five factors. These five factors included two components of MpVLP. The MpVLP deficient in the two factors prepared from the knockdown wasps was less effective than the wild-type MpVLP in terms of the inhibitory activity against host hemocyte spreading estimated in vitro.
言語 en
出版者
出版者 Springer
言語 en
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1007/s13355-016-0476-6
ISSN
収録物識別子タイプ PISSN
収録物識別子 0003-6862
書誌情報 en : Applied Entomology and Zoology

巻 52, 号 2, p. 271-285, 発行日 2017-05
著者版フラグ
値 author
URI
識別子 https://doi.org/10.1007/s13355-016-0476-6
識別子タイプ DOI
URI
識別子 http://hdl.handle.net/2237/26859
識別子タイプ HDL
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